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1999 Fiscal Year Final Research Report Summary

Molecular epidemiology of Borrelia burgdorferi sensu lato in the East Asia.

Research Project

Project/Area Number 10041204
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionUniversity of Shizuoka

Principal Investigator

MASUZAWA Toshiyuki  University of Shizuoka, Microbiology, Associate Professor, 薬学部, 助教授 (10181645)

Co-Investigator(Kenkyū-buntansha) MIYAKE Masaki  University of Shizuoka, Microbiology, Research Associate, 薬学部, 助手 (00295560)
KAWABATA Masato  Kobe University, International Center, Professor, 医学部, 教授 (30175294)
TAKADA Nobuhiro  Fukui Medical University, Medical Zoology, Associate Professor, 医学部, 助教授 (90003409)
Project Period (FY) 1998 – 1999
KeywordsLyme disease / Borrelia / Ixodid tick / Systematic classification / Borrelia burgdorferi / Borrelia afzelii / Borrelia garinii / Borrelia valaisiana
Research Abstract

We conducted a survey to isolate Borrelia from ticks and wild rodents in far eastern Russia, China, and Korea between 1996 and 1998. These isolates were characterized and identified by various molecular typing methods and sequencing analysis. In northeastern China and far eastern Russia, Ixodes persulcatus was a prominent vector and carried B. afzelii and B. garinii. The far eastern type of B. garinii was the dominant species in this area but was not found in Europe, similar to the distribution seen in Hokkaido, Japan and northern part of the South Korea. The finding indicate the transmission of Borrelia between Japan, and far eastern Russia and northeastern China. However, one difference should be point out ; in northern part of South Korea, I. nipponensis has the ability to transmit B. afzelii and B. garinii. Interestingly, isolates from the ticks collected in the southern tip of the Korean Peninsula were identified as B. valaisiana.
In southern China beside the Yangze river valley, Borrelia was isolated from I. granulatus, I. ovatus, and Apodemus agrarius and Niviventer species. Most isolates were identified as B. valaisiana and were similar to the isolates from Korea. However, the group of Borrelia has different type of ospA in comparison with those of European B. valaisiana, indicating that the Borrelia species is divergent. Furthermore, some isolates from Sichuan and Anhui provinces were unique and 16S rRNA gene sequences of these Borrelia species showed similarity values ranging from 99.8 to 100% and clustered with each other on phylogenetic tree, but they were less than 99.0 with those of previously described Lyme disease Borrelia species. Therefore, we speculated that the B. valaisiana found in I. ricinus in Europe was adapted to the tick I. granulatus in central China, I. nipponensis in the southern tip of Korea, and I. columnae in Japan. Furthermore, unique Borrelia species are present in central China.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kaneda, K. et al.: "Infectivity and arthritis induction of Borrelia japonica on SCID mice and immune competent mice : possible role of galactosylceramide binding activity on initiation of infection"Microbilo. Immunol. 42. 173-175 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takada, N. et al.: "Lyme disease spirochetes in ticks from northeastern China"J. parasitol.. 83. 499-504 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li. M. Q. et al.: "Lyme disease Borrelia species in Northeastern China resemble those isolated from far eastern Russia and Japan"Appl. Environ. Microbiol.,. 64. 2705-2709 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野泰弘他: "マダニ幼虫の人体多数寄生に関する疫学的考察"日本ダニ学会誌. 7. 145-148 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢島あゆみ他: "刺し口の周囲にしびれ感を伴ったオーストラリア毒マダニ刺咬症の1例"皮膚. 40. 399-402 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuzawa, T. et al.: "Determination of members of a Borrelia afzelii-related group isolated from Ixodes nipponensis in Korea as Borrelia valaisiana"Int. J. Syst. Bacteriol.. 49. 1409-1416 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishiguro, F. et al.: "Prevalence of Lyme disease Borrelia spp. In ticks from migratory birds on the Japanese mainland"Appl. Environ. Microbiol.,. 66(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柳原保武、増澤俊幸、金田一秀: "特集 Emerging and Re-emerging Infectious Disease 「ライム病(ライムボレリア症)」"Pharma Medica.. 16. 47-53 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸: "ライム病の診断"感染症と化学療法. 4. 37-45 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸: "特集 新興・再興感染症 「ライム病」"CURRENR THERAPY. 17. 331-334 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸: "感染症とその治療-新しい視点から- (I)細菌感染症「日本におけるライム病」"最新医学. 54. 733-740 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸、柳原保武: "エールリッヒア症.エマージングディジーズ"近代出版(東京). 116-121 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸、柳原保武: "ライム病.エマージングディージズ"近代出版(東京). 121-126 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 増澤俊幸: "感染症診断・治療ガイドライン,日本医師会雑誌 増刊"医学書院(東京). 176-179 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柳原保武,増澤俊幸: "ライム病ボレリア.細菌学(竹田美文,林 英夫 編集)"朝倉書店(東京)(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li Muqing, Toshiyuki Masuzawa, Nobuhiro Takada, Fubito Ishiguro, Hiromi Fujita, Atsue Iwaki, Haipeng Wang, Jichun Wang, Masato Kawabata, and Yasutake Yanagihara: "Lyme disease Borrelia species in Northeastern China resemble those isolated from far eastern Russia and Japan."Appl. Environ. Microbiol. 64. 2705-2709 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuhide Kaneda, Toshiyuki Masuzawa, Markus M. Simon, Emiko Isogai, Hiroshi Isogai, Kitsuzo Yasugami, Takashi Suzuki, Yasuo Suzuki, and Yasutake Yanagihara: "Infectivity and arthritis induction of Borrelia japonica on SCID mice and immune competent mice : Possible role of galactosylceramide binding activity on initiation of infection."Microbiol. Immunol.. 42. 171-175 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuhiro Takada, Fubito Ishiguro, Hiromi Fujita, Hai-Peng Wang, Ji-Chun Wang, and Toshiyuki Masuzawa: "Lyme disease spirochetes in ticks from northeastern China"J. Parasitol.. 84. 499-504 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki Masuzawa, Takako Fukui, Masaki Miyake, Hee-Bok Oh, Min-Kee Cho, Woo-Hyun Chang, Yasuyuki Imai, and Yasutake Yanagihara, Y: "Determination of Borrelia afzelii-related group isolated from Ixodes nipponensis in Korea as Borrelia valaisiana."Int. J. Syst. Bacteriol.. 49. 1409-1416 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fubito Ishiguro, Nobuhiro Takada, Toshiyuki Masuzawa, and Takako Fukui: "Prevalence of Lyme disease Borrelia spp. In ticks from migratory birds on the Japanese mainland."Appl. Environ. Microbiol.. (in press). (66)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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