1998 Fiscal Year Annual Research Report

アジアにおけるHIV・肝炎ウイルス感染症の疫学及び分子疫学に関する調査研究

Research Project

Project/Area Number	10041212
Research Category	Grant-in-Aid for international Scientific Research
Section	Field Research
Research Institution	National Institute of Infectious Diseases
Principal Investigator	武部豊国立感染症研究所, エイズ研究センター, 室長 (50126116)
Co-Investigator(Kenkyū-buntansha)	保富康宏三重大学, 医学部, 助教授 (90281724) 滝口雅文熊本大学医学部, エイズ学研究センター, 教授 (00183450) 塩田達雄東京大学医科学研究所, 感染症研究部, 助教授 (00187329) 山本直彦名古屋大学, 医学部, 助教授 (40220488) 阿部賢治国立感染症研究所, 感染病理部, 主任研究官 (60130415)
Keywords	アジア / エイズ / HIV / HVB / HCV / 分子疫学 / サーベーランス
Research Abstract	(1) ミャンマーにおけるHIV流行の分子疫学ミャンマーの首都ヤンゴンで収集した30の全血検体と全国で収集した459の血清サンプルの基づいて解析を進め、その結果ミャンマーでの流行が次の3つのパターンに分類できることを明らかにした。(1) 首都ヤンゴンでは、特にIDUを中心にHIVサブタイプBが、また(2) 性感染者の多いタイ国境に隣接する東南部地域ではサブタイプEが広く分布している。一方、(3) 中部・東北部では、両サブタイプが、IDUおよび性感染者の両者に混在して分布していることを明らかにした。また,血清学的にサブタイピングができない検体が全体の20-30%を占め,他のサブタイプの存在が疑われた。これらの結果は、周辺諸国における流行との密接な関連性を明らかにするもので、将来のワクチン開発に有用な情報を与えるものであった。 (2) アジア地域におけるC型およびG型肝炎ウイルス(HCVおよびHGV)の侵淫状況とその分子疫学的解析東南アジア地域でのHCVおよびHGVのRNA陽性率が、いずれも高い水準にあることを明らかにした。またHCVやHGV感染の危険因子として米国ではIVDUが重要であるのに対し、アジアでは性行為が重要であることが推測された。HGVのゲノタイプ分類の結果、3型(アジア型)が多く、2型(欧米型)は少数(2例)で、1型(西アフリカ型)は認めなかった。ベトナム3例ミャンマー1例ににいずれの型にも分類されない新しいゲノタイプ(4型)を発見した。この他、中国、カンボジア、ベトナム、インドなどアジアにおける新たなフィールドでの調査・研究が展開中であり、研究結果が集積しつつある。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] S.Kusagawa., Y,Takebe., et al.: "Genitic and serologic characterization of HIV type 1 prevailing in Myanmar(Burma)." AIDS Research & Human Retroviruses. 15. 91-94 (1998)
[Publications] S.Kusagawa., Y,Takebe., et al.: "HIV-1 env subtype E in Cambodia." AIDS Research & Human Retroviruses.
[Publications] Y.Fukushima., Y.Takebe., et al.: "Indentifcation and molecular characterzition of human T lymphotropic virus type II infections in intravenous drug abusers in the former South Vietnam." AIDS Research and human letroviruses.14. 537-540 (1998)
[Publications] H.Sato., Y.Takebe., et al.: "Evolution and biological characterization of human immunodificiency virus type 1 subtype E gp120 V3 sequences following horizontal and vetrical virus transmission in a single family." J.Virol.accepted.(1999)
[Publications] H.Sato., Y.Takebe., et al.: "Functional complementation of the envelope hypervariable V3 loop of human immunodeficiency virus type 1 subtype B by subtype E V3 loop. Virology." Virology.(submitted.). (1999)
[Publications] K.Abe, et al.: "Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature." J.of Clinical Microbiology. 36. 3070-3072 (1998)
[Publications] T.Kaneko., K.Abe., et al.: "Molecular cloning of full-lenght sequence of hepatitis G virus genome isolated from a Japanese patient with chronic liver disease" Hepatology Research. 12. 207-216 (1998)
[Publications] Y.Tanaka., M.Mizokami., et al.: "GB virus C/hepatitis G virus infection among Colombian native Indians." Journal of Tropical Medicine & Hygiene.59(3). 462-7 (1998)
[Publications] K.Cao., M.Mizokami., et al.: "GB virus C/hepatitis G virus infection among patients with hepatocellular carcinoma in the inshore area of Yangtze river, China." Journal of Gastroenterology & Hepatology.(In press).
[Publications] S.Kakumu., S.Isomura., et al.: "Prevalence of hepatitis B, hepatitis C, and GB virus C/Hepatitis G virus infections in liver disease patients and inhabitanats in Ho Chi Minh, Vietnam." J.Med.Virol.54. 243-248 (1998)
[Publications] Y.Ikeda., M.Takiguchi., et al.: "Identification of a novel HLA-A24-restricted CTL epitope dirived from HIV-1 Gag protein." AIDS. 12. 2073-2074 (1998)
[Publications] D.Kimieciak., M.Takiguchi., et al.: "The effect of peptide variation on the profile of cytotoxic T lymphocyte responses to the HIV envelope glycoprotein." Internatioal Immunology. 10. 1789-1799 (1998)
[Publications] Y.Chujoh., M.Takiguchi., et al.: "The role of anchor residues in the binding of peptides to HLA-A1101 molecules." Tissue Antigens. 52. 501-509 (1998)
[Publications] H.Tomiyama., M.takiguchi., et al.: "Cytotoxic T-lymphocyte recognition of HLA-B5101-resticted HIV-1 Revepitope which is naturelly processed in HIV-1 infected cells." AIDS. (in press).
[Publications] H.tomiyama., M.Takiguchi., et al.: "Identification of multiple HIV-1 epitopes presented by HLA-B5101 molecules." Human Immunology. (in press).
[Publications] X.Xin., T.Sioda., et al.: "Facilitation of HIV-1 isolation from patjentes by neuraminidase." Arch.Virol.143. 85-95 (1998)
[Publications] A.Kurotani., T.Shioda., et al.: "The paramyxovirus, Sendai virus, C proteins are categorically nonesential gene products but silencing their expression critically impairs viral replication and pathogenecity." Genes Cells. 3. 111-124 (1998)
[Publications] T.Shioda., et al.: "Anti-HIV-1 and chemotactic activities of human SDF-1α and SDF-1β are abolished by CD26/dipeptidyl peptidase IV mediated cleavage." Proc.Natl.Acad.Sci.USA.95. 6331-6336 (1998)

1998 Fiscal Year Annual Research Report

アジアにおけるHIV・肝炎ウイルス感染症の疫学及び分子疫学に関する調査研究

Principal Investigator

武部 豊 国立感染症研究所, エイズ研究センター, 室長 (50126116)

Research Products

[Publications] S.Kusagawa., Y,Takebe., et al.: "Genitic and serologic characterization of HIV type 1 prevailing in Myanmar(Burma)." AIDS Research & Human Retroviruses. 15. 91-94 (1998)

[Publications] S.Kusagawa., Y,Takebe., et al.: "HIV-1 env subtype E in Cambodia." AIDS Research & Human Retroviruses.

[Publications] Y.Fukushima., Y.Takebe., et al.: "Indentifcation and molecular characterzition of human T lymphotropic virus type II infections in intravenous drug abusers in the former South Vietnam." AIDS Research and human letroviruses.14. 537-540 (1998)

[Publications] H.Sato., Y.Takebe., et al.: "Evolution and biological characterization of human immunodificiency virus type 1 subtype E gp120 V3 sequences following horizontal and vetrical virus transmission in a single family." J.Virol.accepted.(1999)

[Publications] H.Sato., Y.Takebe., et al.: "Functional complementation of the envelope hypervariable V3 loop of human immunodeficiency virus type 1 subtype B by subtype E V3 loop. Virology." Virology.(submitted.). (1999)

[Publications] K.Abe, et al.: "Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature." J.of Clinical Microbiology. 36. 3070-3072 (1998)

[Publications] T.Kaneko., K.Abe., et al.: "Molecular cloning of full-lenght sequence of hepatitis G virus genome isolated from a Japanese patient with chronic liver disease" Hepatology Research. 12. 207-216 (1998)

[Publications] Y.Tanaka., M.Mizokami., et al.: "GB virus C/hepatitis G virus infection among Colombian native Indians." Journal of Tropical Medicine & Hygiene.59(3). 462-7 (1998)

[Publications] K.Cao., M.Mizokami., et al.: "GB virus C/hepatitis G virus infection among patients with hepatocellular carcinoma in the inshore area of Yangtze river, China." Journal of Gastroenterology & Hepatology.(In press).

[Publications] S.Kakumu., S.Isomura., et al.: "Prevalence of hepatitis B, hepatitis C, and GB virus C/Hepatitis G virus infections in liver disease patients and inhabitanats in Ho Chi Minh, Vietnam." J.Med.Virol.54. 243-248 (1998)

[Publications] Y.Ikeda., M.Takiguchi., et al.: "Identification of a novel HLA-A24-restricted CTL epitope dirived from HIV-1 Gag protein." AIDS. 12. 2073-2074 (1998)

[Publications] D.Kimieciak., M.Takiguchi., et al.: "The effect of peptide variation on the profile of cytotoxic T lymphocyte responses to the HIV envelope glycoprotein." Internatioal Immunology. 10. 1789-1799 (1998)

[Publications] Y.Chujoh., M.Takiguchi., et al.: "The role of anchor residues in the binding of peptides to HLA-A1101 molecules." Tissue Antigens. 52. 501-509 (1998)

[Publications] H.Tomiyama., M.takiguchi., et al.: "Cytotoxic T-lymphocyte recognition of HLA-B5101-resticted HIV-1 Revepitope which is naturelly processed in HIV-1 infected cells." AIDS. (in press).

[Publications] H.tomiyama., M.Takiguchi., et al.: "Identification of multiple HIV-1 epitopes presented by HLA-B5101 molecules." Human Immunology. (in press).

[Publications] X.Xin., T.Sioda., et al.: "Facilitation of HIV-1 isolation from patjentes by neuraminidase." Arch.Virol.143. 85-95 (1998)

[Publications] A.Kurotani., T.Shioda., et al.: "The paramyxovirus, Sendai virus, C proteins are categorically nonesential gene products but silencing their expression critically impairs viral replication and pathogenecity." Genes Cells. 3. 111-124 (1998)

[Publications] T.Shioda., et al.: "Anti-HIV-1 and chemotactic activities of human SDF-1α and SDF-1β are abolished by CD26/dipeptidyl peptidase IV mediated cleavage." Proc.Natl.Acad.Sci.USA.95. 6331-6336 (1998)

武部豊国立感染症研究所, エイズ研究センター, 室長 (50126116)