1998 Fiscal Year Annual Research Report

鳥類の性決定分子機構

Research Project

Project/Area Number	10044201
Research Category	Grant-in-Aid for international Scientific Research
Section	Joint Research .
Research Institution	Nagoya University
Principal Investigator	島田清司名古屋大学, 農学部, 教授 (40065579)
Co-Investigator(Kenkyū-buntansha)	小野珠乙信州大学, 農学部, 助教授 (10177264) 原田信広藤田保健衛生大学, 医学部, 教授 (00189705) 齋藤昇名古屋大学, 農学部, 助手 (40211924) 朴民根東京大学, 大学院・理学系研究科, 助教授 (00228694) 友金弘日本獣医畜産大学, 獣医畜産学部, 教授 (30023493)
Keywords	ニワトリ / in situ hybridization / PCR / アロマターゼ / 抗ミュラー管ホルモン / アンドロジェンレセプター / 性分化 / 生殖腺
Research Abstract	本研究は、鳥類の性決定や性分化の機構を明らかにすることを目的としている。鶏初期胚の性腺おけるステロイド合成酵素と抗ミューラー管ホルモン(AMH)のmRNA発現の局在性と発現量の推移をin situ hybridizationを用いて調べた。P450_<17α>-hydroxylase(c17)は、雌雄どもに5日から検出され雌では6-7日胚で一時的に増加したがその後減少した。P450aromatase(arom)は、雌では6日から検出されたが、雄では検出されなかった。AMHは、5日から検出され6日から急激に増加したが、雌では6日目から検出ざれたが発現量は低かった。エストラジオールを3日胚に投与すると、雄胚でarom遺伝子が8日から検出された。AMHでは、雄で6日胚で観察されたが、発現量が少なく8日胚では観察出来なかった。以上のように、エストラジオール投与により雄の性腺のAMHが抑制され、aromの発現が誘起された。以上の結果から、性決定に関わる遺伝子は、5日胚以前に重要な役割を演じていることが示された。ニワトリのアンドロジェンレセプターをPCRを用いて、一部をクローニングし塩基配列を決定した。現在、RT-PCRとin situ hybridizationを用いて、初期胚の性腺での発現を調べている。

Research Products
(46 results)

All Other

All Publications (46 results)

[Publications] Abinawanto: "Identification of sperm bearing female-specific chromosome in the sex-reversed chicken." J.Exp.Zool.280. 65-72 (1998)
[Publications] Kansaku N.: "Effects of protein kinase A inhibitor (H-89) on VIP-and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland." Comp.Biochem.Physiol.119C. 89-95 (1998)
[Publications] Matsumoto K.: "Role of retinoic acid in regulation of mRNA expression of CaBP-D28K in the cerebellum of the chicken." Comp.Biochem.Physiol.120A. 237-242 (1998)
[Publications] Kita K,: "Relationship of insulin-like growth factor-I to muscle protein synthesis in fasted chicks." Jpn.Poult Sci.35. 263-270 (1998)
[Publications] Furuse M,: "Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick." Brain Res.807. 214-217 (1998)
[Publications] Kita K,: "Refeeding increases hepatic insuline-like growth factor-I(IGF-I) gene expression and plasma IGF-I concentration in fasted chicks." Brit.Poult.Sci.39. 679-682 (1998)
[Publications] Sasaki T.: "Changes of AVT levels in plasma,neurohypophysis and hypothalamus n relation to oviposition in the laying hen." Comp.Biochem.Physiol.121A. 149-153 (1998)
[Publications] Saito N.: "Effects of short-term dehydration on plasma osmolality,levels of arginine vastocin and its hypothalamic gene expression in the laying hen." Comp.Biochem.Physiol.121A. 235-239 (1998)
[Publications] Furuse M.: "Influence of intracerebroventricular administration of exendin(9-39)on food intake of the newly-hatched chick" Jpn.Poult.Sci.35. 376-380 (1998)
[Publications] Ieda T.: "Effects of aminoglutethimide on levels of messenger ribonucleic acid of CaBP-D28K and of vitamin D3 receptor in the shell gland of laying hen" Jpn.Poult.Sci. 35. 346-355 (1998)
[Publications] Shimada K.: "Gene expression of steroidogenic enzymes in chicken gonads." J.Exp.Zool.281. 450-456 (1998)
[Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing bormone receptor,P450 17α-hydroxylase and P450 aromatase in isolated grnulosa,theca interna and theca externa layers of chicken ovarian follicles during follicular growth" Animal Science Journal. 70. 12-17 (1999)
[Publications] Iizuka A.: "Changes in the expression pattern of luteinizing hormone receptor mRNA in rat testis during degeneration of seminiferous epithelium" Zool.Sci. 15. 255-261 (1998)
[Publications] Mochizuki Y.: "Formation of lipofuscin-like autofluorecent materials in NG108-15 cells:involvement of lysosomal protein degradation" Gerontology. 44. 1-8 (1998)
[Publications] Akazome Y.: "Evidence of sex reversal in the gonads of chicken embryo after oestrogen treatfment as detected by expression of lutropin receptor" J.Reprod.Fert.115. 9-14 (1999)
[Publications] Ohboshi S: "Ultrastrucute of in-vitro blastcysts crypreserved by vitrification" Zygote. 6. 17-28 (1998)
[Publications] Ohoboshi S.: "Effects of glucoase and amino acids on in-vitro development of rat preimplation embryos,and morphological featurtesw of the developed blastcysts." Animal Science and Technology. 69. 637-645 (1998)
[Publications] Harada N.: "Inhibitors of aromatase prevent degaradation of the enzyme in cultured human tumor cells." Br.J.Cancer. 77. 567-572 (1998)
[Publications] Harada N.: "Localized aberrant expression ofr cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver" J.Clin.Endocrinol.Metab.83. 697-702 (1998)
[Publications] Sasano H.: "Aromatase in human central nervous system" Clin.Endocrinol.48. 325-329 (1998)
[Publications] Balthazart J.: "Anatom ical relationship between aromatase and tyrosine hydroxylase in the quail brain:double-label immunocytochemical studies" J.Comp.Neurol. 391. 214-226 (1999)
[Publications] Aste N.,: "districution and effects of testosterone on aromatase mRNA in the quail forebrain: a non-radioactive in situ hybridization study" J.Chem.Neuroanat.14. 103-115 (1998)
[Publications] Sasano H.: "From endocrinology to incrinology" Endocrine Pathology. 9. 9-20 (1998)
[Publications] Sasano H.: "Ontratumoral aromatase in human breasent,endometrial and ovarian malignances" Endocrine Rev.19. 593-607 (1998)
[Publications] Foidart A.: "Neuroanatomical distribution and variations across the reproductive cycle of aromatase activity and aromatase-immunoreactive cells in the pied flycatcher (Ficedula hypoleuca)." Horm.Behav. 83. 697-702 (1998)
[Publications] Nomura M,: "Adrenocortical and gonadal expression of the mammlian Fts-F1 gene encoding Ad4BP/SF-1 is independent of pituitary control." J.Biochem. 124. 217-224 (1998)
[Publications] Harada N.: "Molecular analysis w of aberrent exxpression of aromatasze in breast cancer tissue" Breast Cancer Res.Treat.49. 15-21 (1999)
[Publications] Santen RJ: "Demonstration of aromatase activity and tis regulation in breast tumor and begining breast fibroblasts" Breast Cancer Res.Treat.49. 93-99 (1998)
[Publications] Mor G,: "Macrophages,estrogen and the microemviromental of breast cancer." J.Steroid Bicoehm.Mo.Biol.(in press). (1999)
[Publications] Honda S.: "Disruption of sexual behavior in male aromatase-deficient mice lacking exons 1 and 2 of the cyp19 gene" Biochem.Biophys.Res.Comm.252. 445-449 (1998)
[Publications] 岡本光弘: "動物のステロイドホルモン合成とP450" 化学と生物. 36. 612-618 (1998)
[Publications] 原田信広: "幼少期より多嚢胞性卵巣や骨代謝異常を呈するアロマターゼ完全欠損症の1例" ホルモンと臨床. 46. 215-219 (1998)
[Publications] 原田信広: "アロマターゼ遺伝子" 小児内科. 30. 1038-1041 (1999)
[Publications] Diano S: "Aromatase and estrogen receptor immunoreactivity in the coronary arteries of monkey and human subjects." Menopause. (in press). (1998)
[Publications] Utsumi T: "Steroid sulfate expression is an independent predictor of reccurrence in human breast cancer." Cancer Res.(in press). (1999)
[Publications] Harada N.: "Aromatase inhibitors and enzyme stability" Endocrine-Related Cancer. (in press). (1999)
[Publications] Honda S.: "Identification of cis-acting elements in the proximal promoter region for the brain specific exon 1 of the mouse aromatase gene" Mo.Brain Res.(in press). (1998)
[Publications] Mochii M.: "Spontaneous transdifferentiation of quail pigmented epithelial cell is accompanied by an Mitf mutation." Developmental Biology. 196. 145-159 (1998)
[Publications] Ono,T.: "Settlement of quail primordial germ cells in chicken gonads." Animal Science and Technology. 69. 546-555 (1998)
[Publications] Ono T.: "Production of donor-derived offspring by transfer of primordial germ cells in Japanese quail." Experimental Animals. (in press). (1998)
[Publications] Kino K.: "A low frequency of chromosomal integration of the cloned DNA after microinjection into the cytoplasm of the fertilized eggs of chickens" Japanese Poultry Science. 35. 356-366 (1998)
[Publications] Ono T.: "Transfusion of chick primordial germ cells into quail embryos and their settlement in gonads" Animal Science and Technology. 69. 911-915 (1998)
[Publications] Shimada K.: "Reproductive Biology Update-novel tools for assessment of environmental toxicity" Shoukadoh Booksellers Company, 498 (1998)
[Publications] 朴民根: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 24 (1998)
[Publications] 小林牧人: "「ホルモンの分子生物学」第3巻、生殖とホルモン" 学会出版センター, 57 (1998)
[Publications] Ono,T.: "Methods in Molecular Biology Series,Developmental Biology Protocols (in press)" Humana Press Inc., (1998)

1998 Fiscal Year Annual Research Report

鳥類の性決定分子機構

Principal Investigator

島田 清司 名古屋大学, 農学部, 教授 (40065579)

Research Products

[Publications] Abinawanto: "Identification of sperm bearing female-specific chromosome in the sex-reversed chicken." J.Exp.Zool.280. 65-72 (1998)

[Publications] Kansaku N.: "Effects of protein kinase A inhibitor (H-89) on VIP-and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland." Comp.Biochem.Physiol.119C. 89-95 (1998)

[Publications] Matsumoto K.: "Role of retinoic acid in regulation of mRNA expression of CaBP-D28K in the cerebellum of the chicken." Comp.Biochem.Physiol.120A. 237-242 (1998)

[Publications] Kita K,: "Relationship of insulin-like growth factor-I to muscle protein synthesis in fasted chicks." Jpn.Poult Sci.35. 263-270 (1998)

[Publications] Furuse M,: "Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick." Brain Res.807. 214-217 (1998)

[Publications] Kita K,: "Refeeding increases hepatic insuline-like growth factor-I(IGF-I) gene expression and plasma IGF-I concentration in fasted chicks." Brit.Poult.Sci.39. 679-682 (1998)

[Publications] Sasaki T.: "Changes of AVT levels in plasma,neurohypophysis and hypothalamus n relation to oviposition in the laying hen." Comp.Biochem.Physiol.121A. 149-153 (1998)

[Publications] Saito N.: "Effects of short-term dehydration on plasma osmolality,levels of arginine vastocin and its hypothalamic gene expression in the laying hen." Comp.Biochem.Physiol.121A. 235-239 (1998)

[Publications] Furuse M.: "Influence of intracerebroventricular administration of exendin(9-39)on food intake of the newly-hatched chick" Jpn.Poult.Sci.35. 376-380 (1998)

[Publications] Ieda T.: "Effects of aminoglutethimide on levels of messenger ribonucleic acid of CaBP-D28K and of vitamin D3 receptor in the shell gland of laying hen" Jpn.Poult.Sci. 35. 346-355 (1998)

[Publications] Shimada K.: "Gene expression of steroidogenic enzymes in chicken gonads." J.Exp.Zool.281. 450-456 (1998)

[Publications] Kansaku N.: "Expression of messenger ribonucleic acids of luteinizing bormone receptor,P450 17α-hydroxylase and P450 aromatase in isolated grnulosa,theca interna and theca externa layers of chicken ovarian follicles during follicular growth" Animal Science Journal. 70. 12-17 (1999)

[Publications] Iizuka A.: "Changes in the expression pattern of luteinizing hormone receptor mRNA in rat testis during degeneration of seminiferous epithelium" Zool.Sci. 15. 255-261 (1998)

[Publications] Mochizuki Y.: "Formation of lipofuscin-like autofluorecent materials in NG108-15 cells:involvement of lysosomal protein degradation" Gerontology. 44. 1-8 (1998)

[Publications] Akazome Y.: "Evidence of sex reversal in the gonads of chicken embryo after oestrogen treatfment as detected by expression of lutropin receptor" J.Reprod.Fert.115. 9-14 (1999)

[Publications] Ohboshi S: "Ultrastrucute of in-vitro blastcysts crypreserved by vitrification" Zygote. 6. 17-28 (1998)

[Publications] Ohoboshi S.: "Effects of glucoase and amino acids on in-vitro development of rat preimplation embryos,and morphological featurtesw of the developed blastcysts." Animal Science and Technology. 69. 637-645 (1998)

[Publications] Harada N.: "Inhibitors of aromatase prevent degaradation of the enzyme in cultured human tumor cells." Br.J.Cancer. 77. 567-572 (1998)

[Publications] Harada N.: "Localized aberrant expression ofr cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver" J.Clin.Endocrinol.Metab.83. 697-702 (1998)

[Publications] Sasano H.: "Aromatase in human central nervous system" Clin.Endocrinol.48. 325-329 (1998)

[Publications] Balthazart J.: "Anatom ical relationship between aromatase and tyrosine hydroxylase in the quail brain:double-label immunocytochemical studies" J.Comp.Neurol. 391. 214-226 (1999)

[Publications] Aste N.,: "districution and effects of testosterone on aromatase mRNA in the quail forebrain: a non-radioactive in situ hybridization study" J.Chem.Neuroanat.14. 103-115 (1998)

[Publications] Sasano H.: "From endocrinology to incrinology" Endocrine Pathology. 9. 9-20 (1998)

[Publications] Sasano H.: "Ontratumoral aromatase in human breasent,endometrial and ovarian malignances" Endocrine Rev.19. 593-607 (1998)

[Publications] Foidart A.: "Neuroanatomical distribution and variations across the reproductive cycle of aromatase activity and aromatase-immunoreactive cells in the pied flycatcher (Ficedula hypoleuca)." Horm.Behav. 83. 697-702 (1998)

[Publications] Nomura M,: "Adrenocortical and gonadal expression of the mammlian Fts-F1 gene encoding Ad4BP/SF-1 is independent of pituitary control." J.Biochem. 124. 217-224 (1998)

[Publications] Harada N.: "Molecular analysis w of aberrent exxpression of aromatasze in breast cancer tissue" Breast Cancer Res.Treat.49. 15-21 (1999)

[Publications] Santen RJ: "Demonstration of aromatase activity and tis regulation in breast tumor and begining breast fibroblasts" Breast Cancer Res.Treat.49. 93-99 (1998)

[Publications] Mor G,: "Macrophages,estrogen and the microemviromental of breast cancer." J.Steroid Bicoehm.Mo.Biol.(in press). (1999)

[Publications] Honda S.: "Disruption of sexual behavior in male aromatase-deficient mice lacking exons 1 and 2 of the cyp19 gene" Biochem.Biophys.Res.Comm.252. 445-449 (1998)

[Publications] 岡本光弘: "動物のステロイドホルモン合成とP450" 化学と生物. 36. 612-618 (1998)

[Publications] 原田信広: "幼少期より多嚢胞性卵巣や骨代謝異常を呈するアロマターゼ完全欠損症の1例" ホルモンと臨床. 46. 215-219 (1998)

[Publications] 原田信広: "アロマターゼ遺伝子" 小児内科. 30. 1038-1041 (1999)

[Publications] Diano S: "Aromatase and estrogen receptor immunoreactivity in the coronary arteries of monkey and human subjects." Menopause. (in press). (1998)

[Publications] Utsumi T: "Steroid sulfate expression is an independent predictor of reccurrence in human breast cancer." Cancer Res.(in press). (1999)

[Publications] Harada N.: "Aromatase inhibitors and enzyme stability" Endocrine-Related Cancer. (in press). (1999)

[Publications] Honda S.: "Identification of cis-acting elements in the proximal promoter region for the brain specific exon 1 of the mouse aromatase gene" Mo.Brain Res.(in press). (1998)

[Publications] Mochii M.: "Spontaneous transdifferentiation of quail pigmented epithelial cell is accompanied by an Mitf mutation." Developmental Biology. 196. 145-159 (1998)

[Publications] Ono,T.: "Settlement of quail primordial germ cells in chicken gonads." Animal Science and Technology. 69. 546-555 (1998)

[Publications] Ono T.: "Production of donor-derived offspring by transfer of primordial germ cells in Japanese quail." Experimental Animals. (in press). (1998)

[Publications] Kino K.: "A low frequency of chromosomal integration of the cloned DNA after microinjection into the cytoplasm of the fertilized eggs of chickens" Japanese Poultry Science. 35. 356-366 (1998)

[Publications] Ono T.: "Transfusion of chick primordial germ cells into quail embryos and their settlement in gonads" Animal Science and Technology. 69. 911-915 (1998)

[Publications] Shimada K.: "Reproductive Biology Update-novel tools for assessment of environmental toxicity" Shoukadoh Booksellers Company, 498 (1998)

[Publications] 朴 民根: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 24 (1998)

[Publications] 小林牧人: "「ホルモンの分子生物学」第3巻、生殖とホルモン" 学会出版センター, 57 (1998)

[Publications] Ono,T.: "Methods in Molecular Biology Series,Developmental Biology Protocols (in press)" Humana Press Inc., (1998)

島田清司名古屋大学, 農学部, 教授 (40065579)

[Publications] 朴民根: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 24 (1998)