2000 Fiscal Year Annual Research Report

鳥類の性決定分子機構

Research Project

Project/Area Number	10044201
Research Institution	NAGOYA UNIVERSITY
Principal Investigator	島田清司名古屋大学, 大学院・生命農学研究科, 教授 (40065579)
Co-Investigator(Kenkyū-buntansha)	原田信弘藤田保健衛生大学, 医学部, 教授 (00189705) 朴民根東京大学, 大学院・理学系研究科, 助教授 (00228694) 友金弘日本獣医畜産大学, 獣医畜産学部, 教授 (30023493) 斎藤昇名古屋大学, 大学院・生命農学研究科, 助教授 (40211924) 小野珠乙信州大学, 農学部, 助教授 (10177264)
Keywords	ニワトリ胚 / 性決定 / mRNA / Dmert-1 / Sf-1 / Sox-9 / Dax-1 / Z性染色体
Research Abstract	哺乳類においてY染色体上に位置するSRY(Sex determining region on Y)遺伝子が精巣決定に重要であることが1990年に報告され、高等動物における性決定機構解明のブレークスルー到来と考えられたが、ヒトの性転換に対しSRYで説明できない症例も多く、いくつかの間連遺伝子(Sox9,SF-1,WT-1,DAX-1,Wnt4など)が報告されるようになっている。また、哺乳類以下の動物にはSRYホモローグがみつからないことから、SRYは、進化の過程で哺乳類に特別に発生した遺伝子と推測され、このことは同時に、性決定機構と呼ぶにふさわしい普遍的な機構が別に存在することを示唆する。鳥類は哺乳類と同じように性染色体の対合が性の決定に重要な意味を持っているが、ホモ型、ZZ性染色体の胚は雄にヘテロ型Z・W性染色体の胚は雌に決定される。最近、センチュウやショウジョウバエの性決定に重要とされるmab-3とdoublesex(dsx)遺伝子が明らかになり、これらの相同遺伝子Dmrt-1が魚類、爬虫類、鳥類、哺乳類でも発見され、精巣決定に重要な役割を果たしていることが報告されている。特に、鳥類では、Dmrt-1はZ染色体上にあることからgene dosage effectによって雄が決定されるとする提案は魅力的である。しかしながら、本研究においては、Dmrt-1を始め、SF-1,Sox9,Dax1遺伝子発現が鶏胚の性分化に明確な性差を示したといえる証拠は得られなかった。

Research Products
(34 results)

All Other

All Publications (34 results)

[Publications] Tan FI,Lolait SJ,Brownstein MJ,Saito N,MacLeod V,Baeyens DA,Mayeaux PR,.....: "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken."Biology of Reproduction . 62. 8-15 (2000)
[Publications] Nishikimi H.,Kansaku N.,Saito N.,Usami M.,Ohno Y.and Shimada K.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken."Molecular Reproduction and Development . 55. 20-30 (2000)
[Publications] Suzuki T,Kurosaki T,Agata T,Koide M,Shimada K,Kansaku N,Koide M,.....: "Cytogenetical assignment of 29 functional genes on chicken microchromosomes by FISH."Cytogenetics Cell Genetics. 87. 232-237 (2000)
[Publications] Ieda T.,Takahashi T.,Saito N.,Yasuoka T.,Kawashima M.,and Shimada K.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle."General and Comparative Endocrinology. 117. 182-188 (2000)
[Publications] Sasaki T.,Shimada K.and Saito N.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen."Journal of Experimental Zoology. 286. 481-486 (2000)
[Publications] Sasaki T.,Arakawa K.,Shimada K.and Saito N.: "The effcts of highly selective opioid receptor antagonists on the release of arginine vasotocin induced by hyperosmotic stimulation and angiotensin II injeciton."General and Comparative Endocrinology. 118. 365-372. (2000)
[Publications] Mao X.,Zhang C.,Saito N.,Shimada K.: "Expression of messenger ribinucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in the embryonic and posthatch gonads of the chicken."Japanese Poultry Science. 37. 212-220 (2000)
[Publications] Zheng W.M.,Izaki J.,Furusawa S.and Yoshimura Y.: "Localization of immunoglobulin Gγ-chain mRNA-expressing cells in the oviduct of laying and diethylstilbestrol-treated immature hens."General and Comparative Endocrinology. (in press). (2000)
[Publications] Barua A.and Yoshimura Y.: "Ovarian autoimmunity and its relation to egg production in laying hens."Reproduction . 121. 117-122 (2000)
[Publications] Zheng W.M.and Yoshimura Y.: "Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken."Journal of Poultry Science . (in press). (2000)
[Publications] Yoshimura Y.,Tamura Y.,Nishikoori M.and Okamoto T.: "Effects of diethylstilbestrol intake during growing phase on the reproductive organs in Japanese quail (Coturnix coturnix)."Japanese Poultry Science . (in press). (2000)
[Publications] Isobe N.and Yoshimura Y.: "Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows."Theriogenology. 54. 1159-1169 (2000)
[Publications] Barua A.,Furusawa S.and Yoshimura Y.: "Influence of aging and estrogen treatment on the IgY concentration in the egg yolk of chicken,"Japanese Poultry Science. 37. 280-288 (2000)
[Publications] Isobe N.and Yoshimura Y.: "Localization of apoptotic cells in the cystic ovarian follicles of cows : a DNA-end labeling histochemical study."Theriogenology. 53. 897-904 (2000)
[Publications] Yoshimura Y.,Koike K.and Okamoto T.: "Immunolocalization of progesterone and estrogen receptors in the sperm storage tubules of laying and diethylstilbestrol-injected immature hens."Poultry Science. 78. 94-98 (2000)
[Publications] Hori T.,Asakawa S.,Itoh Y.,Shimizu N.and Mizuno S.: "Wpkci,encoding an altered form of PKCI, is conserved widely on the avian W chromosome and expressed in early female embryos : Implication of its role in female sex determination."Molecular Biology and Cell. 11. 3645-3660 (2000)
[Publications] Takeyama J,Suzuki T,Hirasawa G,Muramatsu Y,Nagura H,Iimura K,......: "17β-Hydroxysteroid dehydrogenase type 1 and 2 expression in the human fetus."Journal of Clinincal Endocrinology and Metabolite . 85. 410-416 (2000)
[Publications] Utsumi T.,Yoshimura N.,Maruta M.,Mizoguchi Y.and Harada N.: "Correlation of cyclin D1 mRNA levels with clinicopathological parameters and clinical outcome in human breast carcinoma. "International Journal of Cancer. 89. 39-43 (2000)
[Publications] Kristensen VN,Harada N,Yoshimura N,Haraldsen E,Lonning PE,Erikstein B,......: "Genetic variations of Cyp19 (aromatase) and breast cancer risk."Oncogene . 19. 1329-1333 (2000)
[Publications] Utsumi T.,Yoshimura N.,Takeuchi S.,Maruta M.,Maeda K.and Harada N.: "Elevated steroid sulfatase expression in breast cancers."Jorunal of Steroid Biochemisty and Molecular Biology. 73. 141-145 (2000)
[Publications] Ohta Y.,Kongo M.,Sasaki E.,Ishiguro I.and Harada N.: "Protective effect of melatonin against α-naphthylisothiocyanate-induced liver injury in rats."Journal of Pineal Reserach. 29. 15-23 (2000)
[Publications] Evrard H.,Baillien M.,Foidart A.,Absil P.,Harada N.and Balthazart J.: "Localization and controls of aromatase in the quail spinal cord."Journal of Comparative Neurology. 423. 552-564 (2000)
[Publications] Balthazart J.,Tlemcani O.,Harada N.and Baillien M.: "Ontogeny of aromatase and tyrosine hydroxylase activity and of aromatase-immunoreactive cells in the preoptic area of male and female Japanese quail."Journal of Neuroendocrinology . 12. 853-866 (2000)
[Publications] Kanda I.,Akazome Y.,Ogasawara O.and Mori T.: "Expression of cytochrome P450 cholesterol side chain cleavage and 3β-hydroxysteroid dehydrogenase during embryogenesis in chicken adrenal glands and gonads."General and Comparative Endocrinology . 118. 96-104 (2000)
[Publications] Sakamoto H.,Ubuka T.,Kohchi C.,Li D.,Ukena K.and Tsutsui K.: "Existence of galanin in lumbosacral sympathetic ganglionic neurons that project to the quail uterine oviduct."Endocrinology . 141. 4402-4412 (2000)
[Publications] Kawai Y.,Ohboshi S.,Yoshida T.and Tomogane H.: "Comparison of the extent of DNA damage in rat embryos developed in vivo and in vitro."Animal Science Journal . 71. 425-427 (2000)
[Publications] Ohkubo T.,Tanaka M.and Nakashima K.: "Molecular cloning of the chicken prolactin gene and activation by Pit-1 and cAMP-induced factor in GH3 cells."General and Comparative Endocrinology . 199. 208-216 (2000)
[Publications] Ohkubo T.,Tanaka M.and Nakashima K.: "Structure and tissue distribution of chicken leptin receptor (cOb-R) mRNA."Biochimistry and Biophysics Acta . 1491. 303-308 (2000)
[Publications] Tanaka M,Yamamoto I,Hayashida Y,Nakao N,Ohkubo T,Wakita M.and Nakashima K.: "Two novel first exons in the prolactin receptor gene are transcribed in a tissue-specific and sexual maturation-dependent manner to encode multiple 5'-truncated transcripts in the testis of the chicken."Biochimistry and Biophysics Acta. 1491. 279-284 (2000)
[Publications] Tsutsui K,Saigoh E,Ukena K,Teranishi H,Fujisawa Y,Kikuchi M,Ishii S.and Sharp PJ.: "A novel avian hypothalamic peptide inhibiting gonadotropin release."Biochemistry and Biophysics Research Communication. 275. 661-667 (2000)
[Publications] Kohchi C.and Tsutsui K.: "Avian galanin : Cloning of complementary DNAs and characterization of transcripts in different tissues."Journal of Experimental Zoology . 287. 183-190 (2000)
[Publications] Saito N.,Sasaki T.,Furuse M.,Arakawa K.and Shimada K.: "Effects of AVT antisense oliodeoxynucleotides on AVT release induced by hypertonic stimulation in chicks."Comparative Biochemistry and Physiology. 128A. 147-153 (2001)
[Publications] Kawaguchi N.,Ono T.,Mochii M.and Noda M.: "Spontaneous mutation in Mitf gene causes osteopetrosis in Silver homozygote quail."Developmental Dynamics . (in press). (2001)
[Publications] Li H.C.,Matsui K.and Ono T.: "Population of circulating primordial germ cells in early Japanese quail embryos."Journal of Poultry Science. (in press). (2001)

2000 Fiscal Year Annual Research Report

鳥類の性決定分子機構

Principal Investigator

島田 清司 名古屋大学, 大学院・生命農学研究科, 教授 (40065579)

Research Products

[Publications] Tan FI,Lolait SJ,Brownstein MJ,Saito N,MacLeod V,Baeyens DA,Mayeaux PR,.....: "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken."Biology of Reproduction . 62. 8-15 (2000)

[Publications] Nishikimi H.,Kansaku N.,Saito N.,Usami M.,Ohno Y.and Shimada K.: "Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken."Molecular Reproduction and Development . 55. 20-30 (2000)

[Publications] Suzuki T,Kurosaki T,Agata T,Koide M,Shimada K,Kansaku N,Koide M,.....: "Cytogenetical assignment of 29 functional genes on chicken microchromosomes by FISH."Cytogenetics Cell Genetics. 87. 232-237 (2000)

[Publications] Ieda T.,Takahashi T.,Saito N.,Yasuoka T.,Kawashima M.,and Shimada K.: "Changes in parathyroid hormone related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle."General and Comparative Endocrinology. 117. 182-188 (2000)

[Publications] Sasaki T.,Shimada K.and Saito N.: "Regulation of opioid peptides on the release of arginine vasotocin in the hen."Journal of Experimental Zoology. 286. 481-486 (2000)

[Publications] Sasaki T.,Arakawa K.,Shimada K.and Saito N.: "The effcts of highly selective opioid receptor antagonists on the release of arginine vasotocin induced by hyperosmotic stimulation and angiotensin II injeciton."General and Comparative Endocrinology. 118. 365-372. (2000)

[Publications] Mao X.,Zhang C.,Saito N.,Shimada K.: "Expression of messenger ribinucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in the embryonic and posthatch gonads of the chicken."Japanese Poultry Science. 37. 212-220 (2000)

[Publications] Zheng W.M.,Izaki J.,Furusawa S.and Yoshimura Y.: "Localization of immunoglobulin Gγ-chain mRNA-expressing cells in the oviduct of laying and diethylstilbestrol-treated immature hens."General and Comparative Endocrinology. (in press). (2000)

[Publications] Barua A.and Yoshimura Y.: "Ovarian autoimmunity and its relation to egg production in laying hens."Reproduction . 121. 117-122 (2000)

[Publications] Zheng W.M.and Yoshimura Y.: "Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken."Journal of Poultry Science . (in press). (2000)

[Publications] Yoshimura Y.,Tamura Y.,Nishikoori M.and Okamoto T.: "Effects of diethylstilbestrol intake during growing phase on the reproductive organs in Japanese quail (Coturnix coturnix)."Japanese Poultry Science . (in press). (2000)

[Publications] Isobe N.and Yoshimura Y.: "Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows."Theriogenology. 54. 1159-1169 (2000)

[Publications] Barua A.,Furusawa S.and Yoshimura Y.: "Influence of aging and estrogen treatment on the IgY concentration in the egg yolk of chicken,"Japanese Poultry Science. 37. 280-288 (2000)

[Publications] Isobe N.and Yoshimura Y.: "Localization of apoptotic cells in the cystic ovarian follicles of cows : a DNA-end labeling histochemical study."Theriogenology. 53. 897-904 (2000)

[Publications] Yoshimura Y.,Koike K.and Okamoto T.: "Immunolocalization of progesterone and estrogen receptors in the sperm storage tubules of laying and diethylstilbestrol-injected immature hens."Poultry Science. 78. 94-98 (2000)

[Publications] Hori T.,Asakawa S.,Itoh Y.,Shimizu N.and Mizuno S.: "Wpkci,encoding an altered form of PKCI, is conserved widely on the avian W chromosome and expressed in early female embryos : Implication of its role in female sex determination."Molecular Biology and Cell. 11. 3645-3660 (2000)

[Publications] Takeyama J,Suzuki T,Hirasawa G,Muramatsu Y,Nagura H,Iimura K,......: "17β-Hydroxysteroid dehydrogenase type 1 and 2 expression in the human fetus."Journal of Clinincal Endocrinology and Metabolite . 85. 410-416 (2000)

[Publications] Utsumi T.,Yoshimura N.,Maruta M.,Mizoguchi Y.and Harada N.: "Correlation of cyclin D1 mRNA levels with clinicopathological parameters and clinical outcome in human breast carcinoma. "International Journal of Cancer. 89. 39-43 (2000)

[Publications] Kristensen VN,Harada N,Yoshimura N,Haraldsen E,Lonning PE,Erikstein B,......: "Genetic variations of Cyp19 (aromatase) and breast cancer risk."Oncogene . 19. 1329-1333 (2000)

[Publications] Utsumi T.,Yoshimura N.,Takeuchi S.,Maruta M.,Maeda K.and Harada N.: "Elevated steroid sulfatase expression in breast cancers."Jorunal of Steroid Biochemisty and Molecular Biology. 73. 141-145 (2000)

[Publications] Ohta Y.,Kongo M.,Sasaki E.,Ishiguro I.and Harada N.: "Protective effect of melatonin against α-naphthylisothiocyanate-induced liver injury in rats."Journal of Pineal Reserach. 29. 15-23 (2000)

[Publications] Evrard H.,Baillien M.,Foidart A.,Absil P.,Harada N.and Balthazart J.: "Localization and controls of aromatase in the quail spinal cord."Journal of Comparative Neurology. 423. 552-564 (2000)

[Publications] Balthazart J.,Tlemcani O.,Harada N.and Baillien M.: "Ontogeny of aromatase and tyrosine hydroxylase activity and of aromatase-immunoreactive cells in the preoptic area of male and female Japanese quail."Journal of Neuroendocrinology . 12. 853-866 (2000)

[Publications] Kanda I.,Akazome Y.,Ogasawara O.and Mori T.: "Expression of cytochrome P450 cholesterol side chain cleavage and 3β-hydroxysteroid dehydrogenase during embryogenesis in chicken adrenal glands and gonads."General and Comparative Endocrinology . 118. 96-104 (2000)

[Publications] Sakamoto H.,Ubuka T.,Kohchi C.,Li D.,Ukena K.and Tsutsui K.: "Existence of galanin in lumbosacral sympathetic ganglionic neurons that project to the quail uterine oviduct."Endocrinology . 141. 4402-4412 (2000)

[Publications] Kawai Y.,Ohboshi S.,Yoshida T.and Tomogane H.: "Comparison of the extent of DNA damage in rat embryos developed in vivo and in vitro."Animal Science Journal . 71. 425-427 (2000)

[Publications] Ohkubo T.,Tanaka M.and Nakashima K.: "Molecular cloning of the chicken prolactin gene and activation by Pit-1 and cAMP-induced factor in GH3 cells."General and Comparative Endocrinology . 199. 208-216 (2000)

[Publications] Ohkubo T.,Tanaka M.and Nakashima K.: "Structure and tissue distribution of chicken leptin receptor (cOb-R) mRNA."Biochimistry and Biophysics Acta . 1491. 303-308 (2000)

[Publications] Tsutsui K,Saigoh E,Ukena K,Teranishi H,Fujisawa Y,Kikuchi M,Ishii S.and Sharp PJ.: "A novel avian hypothalamic peptide inhibiting gonadotropin release."Biochemistry and Biophysics Research Communication. 275. 661-667 (2000)

[Publications] Kohchi C.and Tsutsui K.: "Avian galanin : Cloning of complementary DNAs and characterization of transcripts in different tissues."Journal of Experimental Zoology . 287. 183-190 (2000)

[Publications] Saito N.,Sasaki T.,Furuse M.,Arakawa K.and Shimada K.: "Effects of AVT antisense oliodeoxynucleotides on AVT release induced by hypertonic stimulation in chicks."Comparative Biochemistry and Physiology. 128A. 147-153 (2001)

[Publications] Kawaguchi N.,Ono T.,Mochii M.and Noda M.: "Spontaneous mutation in Mitf gene causes osteopetrosis in Silver homozygote quail."Developmental Dynamics . (in press). (2001)

[Publications] Li H.C.,Matsui K.and Ono T.: "Population of circulating primordial germ cells in early Japanese quail embryos."Journal of Poultry Science. (in press). (2001)

島田清司名古屋大学, 大学院・生命農学研究科, 教授 (40065579)