2000 Fiscal Year Annual Research Report

ウズラ雛「刷り込み」学習の遺伝的・神経的基盤

Research Project

Project/Area Number	10044202
Research Institution	NAGOYA UNIVERSITY
Principal Investigator	松島俊也名古屋大学, 大学院・生命農学研究科, 助教授 (40190459)
Co-Investigator(Kenkyū-buntansha)	KABAI Peter 聖イシュトバーン大学, 動物学センター(ハンガリー), 主任研究員筒井和義広島大学, 総合科学部, 教授 (20163842) 青木清上智大学, 理工学部, 教授 (70101029) CSILLAG Andras センメルバイス大学, 医学部解剖学教室(ハンガリー), 準教授
Keywords	脳 / 行動 / 記憶 / インプリンティング / 基底核 / シナプス / 強化学習 / 鳥
Research Abstract	ウズラ雛の認知機能を明らかにすると共に、記憶形成と行動制御の脳内メカニズムを解析するために一連の解析を総合的に展開した。個体レベルの行動学的解析・脳スライス標本を用いた神経生理学的解析・最初期遺伝子発現を指標とした分子レベルの解析・課題遂行中の覚醒自由行動下における大脳単一ニューロン活動の解析を、総合的に行なうことにより、以下の知見を得た。 (1)行動学的研究:新規学習課題の開発と記銘内容の解析受動的回避学習と馴化学習を指標として雛鳥の想起の内容を解析したところ、一義的には対象の色を詳細に記銘し想起していることが判明した。他方、対象の形に対する弁別カテゴリーは単純で、円形の輪郭を持つものに限局していた。さらに、水を報酬とするGO弁別課題・餌を報酬とするGO/NO-GO弁別課題を開発しその記銘内容を解析したところ、色を第一義として位置を第二義とする多元的な記憶を形成していることが判明した。 (2)記憶の素過程に関する生理学的研究:シナプス長期増強と最初期遺伝子発現大脳IMHV領域(哺乳類の皮質連合野に相当)のスライス標本において、局所興奮性シナプスの長期増強と最初期遺伝子発現の間の因果的関連を吟味したが、両者は基本的に独立であることを示唆する結果を得た。さらに、基底核LPO領域において、D1受容体依存のシナプス長期増強とNMDA受容体依存の長期抑圧が同一ニューロンに同時に生起する現象を見出した。 (3)記憶の細胞表現に関するシステム生理学的研究:記憶に基づく予期と注意の符号化自由歩行下のIMHV領域・LPO領域より単一ニューロン活動を導出し、GO弁別課題・GO/NO-GO弁別課題遂行中の関連活動を解析した。IMHV領域からは視覚・聴覚性注意を表現するニューロンが、LPO領域からは報酬評価と報酬予期を表現するニューロンが、それぞれ見出された。さらに、LPO領域のカイニン酸局所破壊は水を報酬とするGO弁別課題の成立を著しく阻害したが、一度形成された弁別課題の遂行に影響を与えず、刷り込み記憶の形成・発現ともにも影響を与えなかった。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Yanagihara,S.,Yagi,T.,and Matsushima,T.: "Distinct mechanisms for expression of Fos-like immunoreactivity and synaptic potentiation in telencephalic hyperstriatum of the quail chick."Brain Research. 779. 240-253 (1998)
[Publications] Yazaki,Y.,Yamamoto,K.,Matsushima,T.,and Aoki,K.: "Non-genomic action of testosterone mediates avian vocal behavior."Proceedings of the Japan Academy (ser.B). 74. 132-135 (1998)
[Publications] Lefebvre,L.,Gaxiola,A.,Dawson,S.,Timmermans,S.,Rozsa,L.and Kabai,P.: "Feeding innovations and forebrain size in Australian birds.,:"Behaviour. 135. 1077-1097 (1998)
[Publications] Vadasz,C.,Sziraki,I.,Sassvari,M.,Kabai,P.,Murhty,L.R.,Saito,M.,and Laszlovszky.I.: "Analysis of the mesotelencephalic dopamine system by quantitavite-trait Iocul introgression."Neurochem.Res.. 23. 1337-1354 (1998)
[Publications] Kalman M,Szekely AD,Csillag A: "Distribution of glial fibrillary acidic protein and vimentin positive structures in the developing chicken brain from hatch to adulthood."Anat.Embryol. 198. 213-235 (1998)
[Publications] Csillag A: "Striato-telencephalic and striato-tegmental circuits : relevance to learning in domestic chicks."Behavioural Brain Research. 98. 227-236 (1999)
[Publications] Kabai P,Liker A,Csillag A: "Methamphetamine-induced stereotypies in newly-hatched decerebrated domestic chicks."Neurochem.Res.. 24. 1563-1569 (1999)
[Publications] Mezey S,Szekely AD,Bourne RC,Kabai P,Csillag A: "Changes in binding to muscarinic and nicotinic cholinergic receptors in the chick telencephalon,following passive avoidance learning."Neurosci.Lett.. 270. 75-78 (1999)
[Publications] Ishikawa,Y.,Koga.K.,and Matsushima T.: "Slowly developing potentiation in goldfish retino-tectal synapses is masked by prior stimulation ; an in vitro study."Comparative Biochemistry and Physiology A. 124. 81-88 (1999)
[Publications] Dicke,U.,Roth,G.,and Matsushima,T.: "Neural substrate for motor control of feeding in Amphibians."Acta Anatomica. 163. 127-143 (1999)
[Publications] Matsuura,A.,Ohno,T.,Matsushima,T.,Namikawa,T.,and Ishikawa,A.: "Delayed development of reflexes and hyperactive locomotion in the spontaneous mutant "Waltzing" of the musk shrew,Suncus murinus."Experimental Animal. 48. 191-197 (1999)
[Publications] 松島俊也: "心とは何か:雛鳥の学習の記銘内容と神経機構"日本比較内分泌学会ニュースレター. 83. 13-19 (1999)
[Publications] Yazaki,Y.,Matsushima,T.,and Aoki,K.: "Testosterone modulates stimulation-induced calling behavior in Japanese quails."Journal of Comparative Physiology A. 184. 13-19 (1999)
[Publications] Sakai,S.,Yanagihara,S.,Kabai,P.,Koga,K.,and Matsushima,T.: "Predisposed memory of shapes in quail chicks."Zoological Science. 17. 1045-1051 (2000)
[Publications] Aoki,M.,Izawa E.-I.,Koga,K.,Yanagihara,S.,and Matsushima,T.: "Accurate visual memory of colors in controlling the pecking behavior of quail chicks."Zoological Science. 17. 1053-1059 (2000)
[Publications] Yanagihara,S.,Koga,K.,and Matsushima,T.: "Expression of transcription factor ZENK (zif-268) in telencephalon of quail chicks after induced seizure and passive avoidance training."Zoological Science. 17. 1221-1229 (2000)
[Publications] Yanagihara,S.,Izawa,E-I.,Koga,K.,and Matsushima,T.: "Reward-related neuronal activities in basal ganglia of domestic chicks."NeuroReport. (in press). (2001)
[Publications] Csillag,A.: "Anatomy of the living human : Atlas of medical imaging."Koenemann,Cologne(Germany). (1999)

2000 Fiscal Year Annual Research Report

ウズラ雛「刷り込み」学習の遺伝的・神経的基盤

Principal Investigator

松島 俊也 名古屋大学, 大学院・生命農学研究科, 助教授 (40190459)

Research Products

[Publications] Yanagihara,S.,Yagi,T.,and Matsushima,T.: "Distinct mechanisms for expression of Fos-like immunoreactivity and synaptic potentiation in telencephalic hyperstriatum of the quail chick."Brain Research. 779. 240-253 (1998)

[Publications] Yazaki,Y.,Yamamoto,K.,Matsushima,T.,and Aoki,K.: "Non-genomic action of testosterone mediates avian vocal behavior."Proceedings of the Japan Academy (ser.B). 74. 132-135 (1998)

[Publications] Lefebvre,L.,Gaxiola,A.,Dawson,S.,Timmermans,S.,Rozsa,L.and Kabai,P.: "Feeding innovations and forebrain size in Australian birds.,:"Behaviour. 135. 1077-1097 (1998)

[Publications] Vadasz,C.,Sziraki,I.,Sassvari,M.,Kabai,P.,Murhty,L.R.,Saito,M.,and Laszlovszky.I.: "Analysis of the mesotelencephalic dopamine system by quantitavite-trait Iocul introgression."Neurochem.Res.. 23. 1337-1354 (1998)

[Publications] Kalman M,Szekely AD,Csillag A: "Distribution of glial fibrillary acidic protein and vimentin positive structures in the developing chicken brain from hatch to adulthood."Anat.Embryol. 198. 213-235 (1998)

[Publications] Csillag A: "Striato-telencephalic and striato-tegmental circuits : relevance to learning in domestic chicks."Behavioural Brain Research. 98. 227-236 (1999)

[Publications] Kabai P,Liker A,Csillag A: "Methamphetamine-induced stereotypies in newly-hatched decerebrated domestic chicks."Neurochem.Res.. 24. 1563-1569 (1999)

[Publications] Mezey S,Szekely AD,Bourne RC,Kabai P,Csillag A: "Changes in binding to muscarinic and nicotinic cholinergic receptors in the chick telencephalon,following passive avoidance learning."Neurosci.Lett.. 270. 75-78 (1999)

[Publications] Ishikawa,Y.,Koga.K.,and Matsushima T.: "Slowly developing potentiation in goldfish retino-tectal synapses is masked by prior stimulation ; an in vitro study."Comparative Biochemistry and Physiology A. 124. 81-88 (1999)

[Publications] Dicke,U.,Roth,G.,and Matsushima,T.: "Neural substrate for motor control of feeding in Amphibians."Acta Anatomica. 163. 127-143 (1999)

[Publications] Matsuura,A.,Ohno,T.,Matsushima,T.,Namikawa,T.,and Ishikawa,A.: "Delayed development of reflexes and hyperactive locomotion in the spontaneous mutant "Waltzing" of the musk shrew,Suncus murinus."Experimental Animal. 48. 191-197 (1999)

[Publications] 松島俊也: "心とは何か:雛鳥の学習の記銘内容と神経機構"日本比較内分泌学会ニュースレター. 83. 13-19 (1999)

[Publications] Yazaki,Y.,Matsushima,T.,and Aoki,K.: "Testosterone modulates stimulation-induced calling behavior in Japanese quails."Journal of Comparative Physiology A. 184. 13-19 (1999)

[Publications] Sakai,S.,Yanagihara,S.,Kabai,P.,Koga,K.,and Matsushima,T.: "Predisposed memory of shapes in quail chicks."Zoological Science. 17. 1045-1051 (2000)

[Publications] Aoki,M.,Izawa E.-I.,Koga,K.,Yanagihara,S.,and Matsushima,T.: "Accurate visual memory of colors in controlling the pecking behavior of quail chicks."Zoological Science. 17. 1053-1059 (2000)

[Publications] Yanagihara,S.,Koga,K.,and Matsushima,T.: "Expression of transcription factor ZENK (zif-268) in telencephalon of quail chicks after induced seizure and passive avoidance training."Zoological Science. 17. 1221-1229 (2000)

[Publications] Yanagihara,S.,Izawa,E-I.,Koga,K.,and Matsushima,T.: "Reward-related neuronal activities in basal ganglia of domestic chicks."NeuroReport. (in press). (2001)

[Publications] Csillag,A.: "Anatomy of the living human : Atlas of medical imaging."Koenemann,Cologne(Germany). (1999)

松島俊也名古屋大学, 大学院・生命農学研究科, 助教授 (40190459)