1999 Fiscal Year Final Research Report Summary
Molecular analysis of endoderm cell differentiation
| Project/Area Number |
10044204
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
TASAKA Masao NARA INSTITUTION OF SCIENCE AND TECHNOLOGY, GRADUATE SCHOOL OF BIOLOGICAL SCIENCES, PROFESSOR, バイオサイエンス研究科, 教授 (90179680)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Arabidopsis thaliana / SGR1 / SCR / sgr mutants / endodermis / gravitropism / radial pattern formation / SGR7 / SHR |
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| Research Abstract |
We have identified the genes which related to the regulation of endoderm cell differentiation in Arabidopsis root hypocotyl and inflorescence stem. These genes are named SGR1/SCR(shoot gravitropism 1/scarecrow) and SGR7/SHR(Shoot gravitropism 7/short root). The SGR1/SHR have been cloned and it encodes a member of GRAS family. In this project, we made clear next 3 points.
1.The shoot gravitropic mutants named sgr1 and sgr7 were allelic to scarecrow and shoot root respectively by genetic and molecular analysis. The expression pattern of SGR1/SCR gene was analyzed by in situ hybridization and expression pattern of marker gene using transgenic line containing SCR promoter :: GFP.
2.SGR7/SHR was cloned. It was a member of same family of SGR1/SCR.
3.The expression pattern of SGR1/SCR was distributed in a double mutant line of pin (pin formed) and sgr4 (shoot gravitropism 4). In this line, the endoderm cell layer in inflorescence stem was increased partially.
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