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1999 Fiscal Year Final Research Report Summary

Embryo transfer/In vitro fertilization of bovine and buffalo

Research Project

Project/Area Number 10044211
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionYamaguchi University

Principal Investigator

SUZUKI Tatsuyuki  Faculty of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (00216409)

Co-Investigator(Kenkyū-buntansha) OTOI Takeshige  Faculty of Agriculture, Yamaguchi University, Associate Professor, 農学部, 助教授 (30311814)
Project Period (FY) 1998 – 1999
Keywordsbovine / buffalo / superovulation / recover of embryos / in vitro fertilization / simple portable CO2 incubator / CR1aa / CR2aa / somatotropin / blastocyst
Research Abstract

Three of bovine and four of buffalo cows were injected by FSH for superovulation treatment. On day 7, embryos were recovered from these treated cows by non-surgical methods. As a results, number of transferable embryos per cows were obtained 6 out of 7.3 for bovine cows and 1.8 out of 2.3 for buffalo cows, respectively. However, number of transferable embryos were extremely reduced after re-treatment of superovulation (1.3 out of 3.7 for bovine and 0.5 out of 1.5 for buffalo cows, respectively).
Buffalo and Bovine cows oocytes were collected for IVM, IVF and IVC. A portable CO2 incubator and CR1aa or CR2aa medium in the presence of somatotropin(GH) were used for in vitro production of embryos. From the present data, 200ng/ml GH in CR1aa medium seems to provide the optimum in vitro production of bovine embryos, although such high a high concentration of GH had no influence in CR2aa medium, may play a key role in subsequent development of embryos in vitro. In fact, we obtained the 40.2% of transferable embryos using CR2aa medium for bovine and buffalo. Also, our improved simple portable incubator can be used successfully for in vitro production of bovine embryos and could be applicable at the field level.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Omaima MK and others: "New technique,Uusing a portable CO2 incubator,for the production of in vitro fertilized egyptian buffalo embyros"J.Reprod.Dev.. 45. 315-320 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abdoon ASS and others: "Factors effecting number of surface antral follicles,quality and quality of oocytes in egyptian buffalo ovaries"Anim.Reprod.Sci.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T and others: "Development of a simple,portable carbon dioxide incubator for in vitro production of bovine embryos"Anim.Reprod.Sci.. 54. 149-157 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dong YJ and others: "Production of bovine IVF enbryos using portable diocide incubator-Effect of naqativc air pessure on embryonic development"Jpn J Fertil Steril. 45. 31-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T. and others.: "n vitro Procduction of bovine embryos inthe presence of growth hormone in CR1aa or CR2aa medium using an improved portable CO2 incubator"Theriogenology. 53. 301-301 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Omaima MK and others: "Effect of increaslng dietary fat on oculation rate,embryo production and some blood biochernical values lin Sheep"Alex.J.Vet.Sci.. 14. 117-129 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] OMAIMA MK and others: "New technique, using a portable CO2 incubator, for the producction of in vitro fertilized egyptian buffalo embryos."J. Reprod. Dev.. 45. 315-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ABDOON ASS and others: "Factors affecting number of surface antral follicles, quantity and quality of oocytes in egyptian buffalo ovaries."Anim. Reprod. Sci.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SUZUKI T and others: "Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos."Anim. Reprod. Sci.. 54. 149-157 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] DONG YJ and others: "production of bovine IVF embryos using portable carbon dioxide incubator-Effect of negative air pressure on embryonic development."Jpn j Fertil Steril. 45. 31-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SUZUKI T and others: "In vitro production of bovine embryos in the presence of growth hormone in CR1aa or CR2aa medium using an improved portable CO2 incubator."Theriogenology. 53. 301 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OMAIMA MK and others: "Effect of increasing dietary fat on oculation rate, embryos production and some blood biochemical values in sheep."Alex. J. Vet. Sci.. 14. 117-129 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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