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2000 Fiscal Year Final Research Report Summary

Joint Study on the protein-kinase-type photoreceptors

Research Project

Project/Area Number 10044214
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionTokyo Metropolitan University

Principal Investigator

WADA Masamitsu  Tokyo Metropolitan University, Biology, Professor, 理学研究科, 教授 (60011681)

Co-Investigator(Kenkyū-buntansha) KANEGAE Takeshi  Tokyo Metropolitan University, Biology, Instructor, 理学研究科, 助手 (70264588)
KADATA Akeo  Tokyo Metropolitan University, Biology, Associate Professor, 理学研究科, 助教授 (60152758)
Project Period (FY) 1998 – 2000
Keywordsphotomorphogenesis / photoreceptor / chloroplast movement / phototropism / fern / gametophyte / 光屈性
Research Abstract

1. Arabidopsis mutants which were deficient of chloroplast photoorientation movement were selected by a new method developed.
2. Analytical studies on the causal gene of the mutants revealed that NPL1, a homologue of NPH1 which is a blue light receptor of phototropism in Arabidopsis, is a photo-receptor of chloroplast avoidance response induced by strong blue light.
3. npl1 and nph1 double mutants lack chloroplast photoorietation movement as well as phototropism, meaning that both NPL1 and NPH1 are the photoreceptor of the two phenomona.
4. NPH1 is a photoreceptor for chloroplast accumulation movement in the whole range of fluence rate tested. On the other hand, NPL1 is a receptor for accumulation under weak blue light but a receptor for avoidance under strong blue light.
5. Adiantum PHY3 binds FMNs at the two LOV domains, suggesting that PHY3 is a possible photoreceptor for blue light as well as red/far-red light.
6. PHY3 was found widely in advanced Pteridophytes but not in the lower ferns.
7. By mutant anlyses and also by gene silencing method, it was found that PHy3 was a photoreceptor for red light-inducedchloroplast movement and phototropism in Adiantum.
8. Adiantum NPL1 was sequenced and found that NPL1 was a photoreceptor of blue light-induced chloroplast avoidance response in Adiantum like in Arabidopsis.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] A.Kadota: "Intracellular chloroplast photorelocation in the moss Physcomitrella patens is mediated by phytochrome as well as by a blue-light receptor."Planta. 210. 932-937 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kanegae: "Rice NDHI homologues, OsNPHIa and OsNPH16, are differently photoregulated."Plant and Cell Physiology. 41. 415-423 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kiyosue: "LKP1 (LOV kelch protein 1) : a factor involved in the regulation of flowering time in Arabidopsis"Plant Journal. 23. 807-815 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Sato: "Choice of trucks, microtubules and/or actin filaments, forchloroplast movement is differently controlled by phytochrome and blue-light-receptor"Journal of Cell Science. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kagawa: "Arabidopsis NPL1 : A phototropin homologue controlling the chloroplast high-light avoidance response"Science. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kadota, A.and M.Wada: "Red light-aphototropic (rap) mutants lack red light-induced chloroplast relocation movement in the fern Adiantum capillus-veneris."Plant Cell Physiol.. 40. 238-247 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagawa, T.and M.Wada: "Chloroplast-avoidance response induced by high-fluence blue light in prothallial cells of the fern Adiantum capillus-veneris as analyzed by microbeam irradiation."Plant Physiol.. 119. 917-923 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imaizumi, T., T.Kiyosue, T.Kanegae and M.Wada: "Cloning of the cDNA encoding the blue-light photoreceptor (cryptochrome) from the moss Physcomitrella patens (Accession No. AB027528)(PGR99-110)"Plant Physiol.. 120. 1205 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Christie, J.M., M.Salomon, K.Nozue, M.Wada and W.R.Briggs: "LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1) : Binding sites for the chromophore flavin mononucleotide."Proc.Natl.Acad.Sci.USA. 96. 8779-8783 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, Y., A.Kadota and M.Wada: "Mechanically-induced avoidance response of chloroplasts in fern protonemal cells."Plant Physiology. 121. 37-44 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazato, T., A.Kadota and M.Wada: "Photoinduction of spore germination in Marchantia polymorpha L.is mediated by photosynthesis."Plant Cell Physiol.. 40. 1014-1020 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imaizumi, T., T.Kanegae and M.Wada: "Cryptochrome nucleocytoplasmic distribution and gene expression are regulated by light quality in the fern Adiantum capillus-veneris."Plant Cell. 12. 81-96 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagawa, T.and M.Wada: "Blue light-induced chloroplast relocation in Arabidopsis thaliana as analyzed by microbeam irradiation."Plant Cell Physiol.. 41. 84-93 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kadota, A., Y.Sato, and M.Wada: "Intracellular chloroplast photorelocation in the moss Physcomitrella patens is mediated by phytochrome as well as by a blue-light receptor."Planta. 210. 932-937 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyosue, T.and M.Wada: "LKP1 (LOV kelch protein 1) : a factor involved in the regulation of flowering time in Arabidopsis."Plant Journal. 23. 807-815 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nozue, K., J.Christie, T.Kiyosue, W.R.Briggs and M.Wada: "Isolation and characterization of fern phototropin (Accession No.AB037188). A putative blue-light photoreceptor for phototropism (PGR00-039)."Plant Physiol.. 122. 1457 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, Y., M.Wada, and A.Kadota: "Choice of tracks, microtubules and/or actin filaments, for chloroplast photomovement is differentially controlled by phytochrome and a blue light receptor."J Cell Sci.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kagawa, T., T.Sakai, N.Suetsugu, K.Oikawa, S.Ishiguro, T.Kato, S.Tabata, K.Okada and M.Wada: "Arabidopsis NPL1 : A phototropin homologue controlling the chloroplast high-light avoidance response"Science.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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