1999 Fiscal Year Final Research Report Summary
Molecular Mechanism of corticohistoqenesis of the brain
| Project/Area Number |
10044245
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIKOSHIBA Katsuhiko The Institute of Medical Science, The University of Tokyo, Department of Molecular Neurobiology, Professor, 医科学研究所, 教授 (30051840)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Masaharu The Institute of Physical and Chemical Research (RIKEN) The team of Development Neurobiology, researcher, 脳科学総合研究センター, チームリーダー(研究職) (50111951)
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| Project Period (FY) |
1998 – 1999
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| Keywords | reelin / CR-50 antibody / cerebral cortex / cortex |
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| Research Abstract |
Reelin is a key mediator of ordered neuronal alignment in the brain. Here we demonstrate that Reelin molecules assemble each other to forms a huge protein complex both in vitro and in vivo. The Reelin-Reelin interaction is clearly inhibited by the function-blocking anti-Reelin antibody, CR-50, at a concentration known to inhibit Reelin function. This assembly is mediated by electrostatic interaction via the α-helix domains in the CR-50 epitope region. Recombinant CR-50 epitope fragments spontaneously constitute a soluble, string-like homopolymer with a regularly repeated structure composed of more than 40 monomers. These data suggest that Reelin exerts its biological function by composing a large protein assembly driven by the CR-50 epitope region, proposing a novel model of the Reelin signaling in neurodevelopment.
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[Publications] Yoshikawa, F., Iwasaki, H., Michikawa, T., Furuichi, T. and Mikoshiba, K.: "Cooperative formation of the ligand-binding site of the inositol 1, 4, 5-trisphosphate receptor by two separable domains."J. Biol. Chem.. 274. 328-334 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshikawa, F., Iwasaki, H., Michikawa, T., Furuichi, T. and Mikoshiba, K.: "Trypsinized cerebellar inositol 1, 4, 5-trisphosphate receptor."J. Biol. Chem.. 274. 316-327 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Zhu, C., Furuichi, T., Mikoshiba, K. and Wojcikiewicz, R. J. H.: "Inosiotl 1, 4, 5-trisphosphate receptor down-regulation is activated directly by inositol 1, 4, 5-trisphosphate binding."J. Biol. Chem.. 274. 3476-3484 (1999)
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[Publications] Yoshikawa, F., Uchiyama, T., Iwasaki, H., Tomomori-Satoh, C., Tanaka, T., Furuichi, T. and Mikoshiba, K.: "High efficient expression of the functional ligand binding site of the inositol 1, 4, 5-trisphosphate receptor in Escherichia coli."Biochem. Biophys. Res. Commun.. 257. 792-797 (1999)
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[Publications] Konishi, Y., Ohkawa, N., Makino, Y., Ohkubo, H., Kageyama, R., Furuichi, T., Mikoshiba, K. and Tamura, T.: "Transcriptional regulation of mouse type 1 inositol 1, 4, 5-trisuphosphate receptor gene by neuroD-related factor."J. Neurochem.. 72. 1717-1724 (1999)
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[Publications] Ohkawa, N. Konishi, Y. Shimada, M. Makino, Y., Yoshikawa, S. Mikoshiba, K. and Tamura, T.: "Activation of the mouse inositol 1, 4, 5-trisphosphate receptor type 1 promoter by AP-2"Gene. 229. 11-19 (1999)
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[Publications] Hamada, T., Liou. S. Y., Fukushima, T., Maruyama, T., Watanabe, S., Mikoshiba, K. and Ishida, N.: "The role of inositol trisphosphate-induced Ca2+ release from IP3-receptor in the rat suprachiasmatic nucleus on circadian entrainment mechanism."Neuroscience. Letters. 263. 125-128 (1999)
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[Publications] Natsume, T., Hirota, J., Yoshikawa, F., Furuichi, T. and Mikoshiba, K.: "Real time analysis of interaction between inositol 1, 4, 5-trisphosphate receptor type 1 and its ligand."Biochem. Biophys. Res. Commun.. 260. 527-533 (1999)
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[Publications] Michikawa, T., Hirota, J., Kawano, S., Hiraoka, M., Yamada, M., Furuichi, T. and Mikoshiba, K.: "Calmodulin mediates calcium-dependent inactivation of the cerebellar type 1 inositoll, 4, 5-trisphosphate receptor."Neuron. 23. 799-808 (1999)
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[Publications] Hirota, J., Michikawa, T., Natsume, T., Furuichi, T. and Mikoshiba, K.: "Calmodulin inhibits inositol 1, 4, 5-trisphosphate-induced calcium release through the purified and reconstituted inositol 1, 4, 5-trisphosphate receptor type 1."FEBS Letters. 456. 322-326 (1999)
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[Publications] Nagata, E., Tanaka, K., Suzuki, S., Dembo, T., Fukuuchi, Y., Futatsugi, A. and Mikoshiba, K.: "Selective inhibition of inostitol 1, 4, 5-trisphosphate-induced Ca2+ release in the CA1 region of the hippocampus in the ischemic gerbil."Neuroscience. 93. 995-1001 (1999)
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[Publications] Hayashi, M., Monkawa, T., Yoshida, T., Sasamura, H., Matsumoto, M., Inoue, T., Mikoshiba, K. and Saruta, T.: "Intracellular calsium concentration in the inositol trisphosphate receptor type 1 knockout mouse."J Am Soc Nephrol. 10. 2094-2101 (1999)
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[Publications] Boulay, G., Brown, D. M., Qin, N., Jiang, M., Dietrich, A., Zhu, M. X., Chen, Z., Bimbaumer, M., Mikoshiba, K. and Bimbaumer, L.: "Modulation of Ca2+ entry by polypeptides of the inositol 1, 4, 5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP) : evidence for roles of TRP and IP3R in store depletion activated Ca2+ entry."Proc. Natl. Acad. Sci. USA. 96. 14955-14960 (1999)
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[Publications] Baylis, H. A., Furuichi, T., Yoshikawa, F., Mikoshiba, K. and Sattelle, D. B.: "Inositol 1, 4, 5-trisphosphate receptors are strongly expressed in the nervous system, pharynx, intestine, gonad and excretory cell of caenorhabditis elegans and are encoded by a single gene (itr-1)."J. Mol. Biol.. 294. 467-476 (1999)
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