1999 Fiscal Year Annual Research Report

細胞周期・増殖を制御するシグナル伝達系の研究

Research Project

Project/Area Number	10044261
Research Institution	Nagoya University
Principal Investigator	浜口道成名古屋大学, 医学部, 教授 (90135351)
Co-Investigator(Kenkyū-buntansha)	松田覚名古屋大学, 医学部, 講師 (50242110) 花房秀三郎大阪バイオサイエンス研究所, 所長(研究者)
Keywords	増殖 / 細胞周期 / チロシンキナーゼ / 増殖制御因子 / 細胞間接着
Research Abstract	本研究は、第一級の国際的な研究者による共同研究のもとに、細胞周期を統御する細胞内シグナル伝達系を、多様な変異遺伝子を用いて解析する事を目的とする。近年の研究から、細胞の増殖は、チロシンリン酸化を介するシグナル伝達系による正の制御と細胞周期制御因子による負の制御からなるシステムよる統御をいけている事が明らかになっている。そこで、本年は、チロシンフォスファターゼSHP-2の結合蛋白質として同定され、まだ十分その機能が明らかになっていないSHPS-1について解析をすすめた。その結果、v-Srcによる癌化は、SHPS-1の発現を抑制する事、SHPS-1の遺伝子をクローニングし、v-Src癌化細胞にに導入する、癌細胞の増殖を選択的に阻害する事が明らかになった。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Serbulea,M.,Hamaguchi,M.et al.: "Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway."Int.J.Oncogene. 14. 733-738 (1999)
[Publications] Akhand,A.A.,Hamaguchi,M.et al.: "Nitric oxide controls Src kinase activity through a redox-based molecular modification."J.Biol.Chem.. 274. 25821-25826 (1999)
[Publications] Thant,A.A.,Hamaguchi,M.: "Ras pathway is required for the activation of MMP-2 and for the invasion of src-transformed 3Y1."Oncogene. 18(47). 6555-6563 (1999)
[Publications] Itano,N.,Hamaguchi,,et al.: "Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties."J.Biol.Chem.. 274. 25085-25092 (1999)
[Publications] Miyazaki,K.,Hamaguchi,M.: "Critical amino acid substitutions in the Src SH3 domain that convert c-Src to be oncogenic."Biochem.Bioph.Res.Co.. 263. 759-764 (1999)
[Publications] Okuda,T.,Hamaguchi,M.et al.: "Molecular cloning of macrophin,a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin."Biochem.Bioph.Res.Co.. 264. 568-574 (1999)
[Publications] Kurata,K.,Hamaguchi,M.: "Constitutive activation of MAP kinase (MEK1) is critical and sufficient for the activation of MMP-2."Exp.Cell.Res.. 254. 180-188 (2000)
[Publications] Senga,T.,Hamaguchi,M.: "Clustered cysteine residues in the kinase domain of v-Src: critical role for protein stability, cell transformation and sensitivity to herbimycin A."Oncogene. 19. 273-279 (2000)
[Publications] Matsuda,S.,Hamaguchi,M.: "Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase."Biochim.Biophys.Acta. 1490. 163-169 (2000)
[Publications] Matsuda,S.,Hamaguchi,M.: "Molecular cloning and characterization of human MAWD,a novel protein containing WD-40 repeats frequently overexpressed in breast cancer."Cancer.Res.. 60. 13-17 (2000)
[Publications] Machida,K.,Hamaguchi,M.: "v-Src suppresses SHPS-1 expression via the Ras-MAP kinase pathway to promote the oncogenic growth of cells."Oncogene. (in press).

1999 Fiscal Year Annual Research Report

細胞周期・増殖を制御するシグナル伝達系の研究

Principal Investigator

浜口 道成 名古屋大学, 医学部, 教授 (90135351)

Research Products

[Publications] Serbulea,M.,Hamaguchi,M.et al.: "Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway."Int.J.Oncogene. 14. 733-738 (1999)

[Publications] Akhand,A.A.,Hamaguchi,M.et al.: "Nitric oxide controls Src kinase activity through a redox-based molecular modification."J.Biol.Chem.. 274. 25821-25826 (1999)

[Publications] Thant,A.A.,Hamaguchi,M.: "Ras pathway is required for the activation of MMP-2 and for the invasion of src-transformed 3Y1."Oncogene. 18(47). 6555-6563 (1999)

[Publications] Itano,N.,Hamaguchi,,et al.: "Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties."J.Biol.Chem.. 274. 25085-25092 (1999)

[Publications] Miyazaki,K.,Hamaguchi,M.: "Critical amino acid substitutions in the Src SH3 domain that convert c-Src to be oncogenic."Biochem.Bioph.Res.Co.. 263. 759-764 (1999)

[Publications] Okuda,T.,Hamaguchi,M.et al.: "Molecular cloning of macrophin,a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin."Biochem.Bioph.Res.Co.. 264. 568-574 (1999)

[Publications] Kurata,K.,Hamaguchi,M.: "Constitutive activation of MAP kinase (MEK1) is critical and sufficient for the activation of MMP-2."Exp.Cell.Res.. 254. 180-188 (2000)

[Publications] Senga,T.,Hamaguchi,M.: "Clustered cysteine residues in the kinase domain of v-Src: critical role for protein stability, cell transformation and sensitivity to herbimycin A."Oncogene. 19. 273-279 (2000)

[Publications] Matsuda,S.,Hamaguchi,M.: "Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase."Biochim.Biophys.Acta. 1490. 163-169 (2000)

[Publications] Matsuda,S.,Hamaguchi,M.: "Molecular cloning and characterization of human MAWD,a novel protein containing WD-40 repeats frequently overexpressed in breast cancer."Cancer.Res.. 60. 13-17 (2000)

[Publications] Machida,K.,Hamaguchi,M.: "v-Src suppresses SHPS-1 expression via the Ras-MAP kinase pathway to promote the oncogenic growth of cells."Oncogene. (in press).

浜口道成名古屋大学, 医学部, 教授 (90135351)