1999 Fiscal Year Final Research Report Summary
Virus Receptors and Cytokines
| Project/Area Number |
10044279
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TASHIRO Kei Kyoto University, Center for Molecular Biology and Genetics Associate Professor, 遺伝子実験施設, 助教授 (10263097)
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| Co-Investigator(Kenkyū-buntansha) |
田代 啓 京都大学, 遺伝子実験施設, 助教授 (10263097)
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| Project Period (FY) |
1998 – 1999
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| Keywords | AIDS / SDF-1 / Cytokine / CXCR4 / HIV-1 / Polymorphysm / receotor / TR-FLA |
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| Research Abstract |
Collaboration was done with Dr.Verma (SALK Institute, U.S.A.), Dr.O'Brien (NCI, U.S.A.), Dr. Durandy (INSERM, France) and their colleagues on a cytokine, SDF-1, which is the natural ligand for CXCR4, the coreceptor for T tropic HIV virus.
1. To understand molecular mechanism of our finding that AIDS onset is delayed in the persons who bear homozygous alleles of SDF1 gene AIDS delaying type polymorphysm, the blood SDF-1 concentration in each individuals bearing each genotype was measured. For that, new measuring system named SDF-1 TR-FIA was developed in collaboration with Dr.Matsumoto (Waseda Univ./CREST) and blood samples were collected by Dr.O'Brien. The measuring work is still on going.
2. In our SDF-1 expression system in vivo, CXCR4 on CD4ィイD1+ィエD1 cells were down-modulated in the presence of excess amount of SDF-1, showing the molecular mechanism of the delay.
3. We are screening additional polymorphic site linked with the SDF1 gene AIDS delaying type polymorphysm.
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