1999 Fiscal Year Final Research Report Summary
Molecular mechanism underlying the embryonic development of adrenal cortex and gonads.
| Project/Area Number |
10044280
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
OKAMOTO Mitsuhiro Osaka University Medical School, Professor, 医学系研究科, 教授 (90028613)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Osamu Nara Medical University, 講師 (40164850)
TAKEMORI Hiroshi Osaka University Medical School, 医学系研究科, 助手 (90273672)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Adrenal cortex / Gonads / Steroid hormones / ZOG protein / Cytochrome P450 |
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| Research Abstract |
(1) The expression of ZOG/Pref-1 was immunohistochemically studied on the rat embryos. It was expressed in the adreno-gonadal primordium on the embryonic day 12, and after the day 14 its expression was restricted only in the adrenocortical primordiumm. In the adult cortex its expression was localized in the zona glomerulosa.
(2) The cell-type-specific expression of ZOG/Pref-1 gene was studied. The results suggested that nuclear factors other than Sp 1/Egr(s) played important roles for the expression.
(3) CYP11B1 was expressed in Escherichia coli and purified. Its structure/function relationship was studied. Two amino acid residues in its active site, V381 and I384, were found to be important to support its versatile catalytic activity.
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[Publications] Halder, S.K., Takemori, H., Hatano, O., Nonaka, Y., Wada, A., and Okamoto, M.: "Cloning of a membrane-spanning protein with epidermal growth factor-like repeat motifs from adrenal glomerulosa cells"Endocrinology. 139. 3316-3328 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Nonaka, Y., Fujii, T., Kagawa, N., Waterman, M.R., Takemori, H., and Okamoto, M.: "Structure/function relationship of CYP11B1 associated with Dahl's salt-resitant rats. Expression of rat CYP11B1 and CYP11B2 in Escherichia coli."Eur. J.Biochem.. 258. 869-878 (1998)
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[Publications] Nonaka, Y., Makino, A., Takemori, H., and Okamoto, M.: "The GroEL and GroES chaperones are major catalysts in mitochondrial CYP11B1-folding in E.coli."Molecular Steroidogenesis (2000) Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H., ed.. 101-102 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Hatano, O., Takemori, H., Okamoto, M., and Tohno, Y.: "Genesis of the adrenal cortex and gonads from their common primordium"Molecular Steroidogenesis Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H., ed.. 197-200 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Takemori, H., Doi, J., Kato, Y., Hatano, O., and Okamoto, M.: "Characterization of proximal element in the rat Pref-1 (ZOG) gene promoter"Molecular Steroidogenesis (2000) Universal Academy Press, Okamoito, M., Ishimura, Y., and Nawata, H., ed.. 209-212 (2000)
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[Publications] Doi, J., Takemori, H., Ohta, M., Nonaka, Y., and Okamoto, M.: "Epidermal growth factor and basic fibroblast growth factor induce 3beta-hydroxysteroid dehydrogenase type II in human adrenocortical carcinoma cells."Molecular Steroidogenesis 2000 Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H., ed.. 277-278 (2000)
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[Publications] Lin, X.-z., Wang, Z.-n., Takemori, H., Katoh, Y., and Okamoto, M.: "Induction of salt inducible kinase (SIK) by ACTH in adrenal cortex."Molecular Steroidogenesis, 2000 Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H.ed.. 279-280 (2000)
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[Publications] Horike, N., Takemori, H., Okamoto, M., and Sonobe, H.: "Developmental change of ecdysone 20-hydroxylase [E20(OH)ase] and NADPH-cytochrome P450 reductase [bCPR] during embryogenesis of silkworm, Bombyx mori"Molecular Steroidogenesis, 2000, . Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H.ed.. 331-332 (2000)
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[Publications] Ohta, M., Takemori, H., Doi, J., Nonaka, Y., and Okamoto, M.: "Highly sensitive atmospheric pressure chemical ionization (APCI)-LC-MS analysis of metabolites produced by steroidogenic cytochrome P450s."Molecular Steroidogenesis, 2000. Universal Academy Press, Okamoto, M., Ishimura, Y., and Nawata, H., ed.. 415-417 (2000)
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