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2000 Fiscal Year Final Research Report Summary

Redox regulation by glutathione and the roles of reactive oxygen and nitrogen species

Research Project

Project/Area Number 10044286
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionOsaka University

Principal Investigator

TANIGUCHI Naoyuki  Osaka University, Graduate School of medicine, Professor, 医学系研究科, 教授 (90002188)

Co-Investigator(Kenkyū-buntansha) FUJII Junichi  Yamagata University, School of Medicine, Professor, 医学部, 教授 (00222258)
SUZUKI Keiichiro  Hyogo College of Medicine, Professor, 教授 (70221322)
HIGASHIYAMA Shigeki  Osaka University, School of Allied Health Sciences, Associate Professor, 医学部, 助教授 (60202272)
IKEDA Yoshitaka  Osaka University, Graduate School of medicine, Assistant Professor, 医学系研究科, 助手 (60252657)
TAKAHASHI Motoko  Osaka University, Graduate School of medicine, Assistant Professor, 医学系研究科, 助手 (00303941)
Project Period (FY) 1998 – 2000
KeywordsReactive oxygen species / glutathine / nitric oxide / redox balance / growth factor / glutathione peroxidase / NO合成酵素
Research Abstract

Reactive oxygen species(ROS) such as the superoxide anion (O_2^-), hydrogen peroxide (H_2O_2) and the hydroxyl radical (OH) are represent putative biohazards when present in excess, a condition which,is referred to as "oxidative stress". ROS are generated within cells as byproducts of biological oxidations and are thought to play important roles in several pathophysiologic events, including inflammation, mutagenesis, and aging. Recently ROS and redox balance are thought to act as an intracellular signal and have a wide variety of biological action. So in this project we have demonstrated new biological functions of ROS, glutathione and redox balance.
1)Inactivation of glutathine peroxidase by reducing sugars and dicarbonyl compounds
2)New biological faction of peroxiredixin family : Purification and cloning of peroxiredoxin IV and its antioxidative property in extracellular space
3)Ceruloplasmin(Cp) has a glutathione peroxidase-like activity together with its ferroxidase activity and would completely remove the primary reactants required for both Fenton chemistry and lipid peroxidation. And Cp binds to extracellular myeloperoxidase(MPO) and prevents MPO making HOC1, a powerful oxidant which will lead to molecular damage.
4)Hydroxyl radicals accelerates the adhesion of neutrophils and cancer cells to endothelial cells in the absence of de novo protein synthesis
5)Mechanisms of anti-oxidative functions of glutathione, giutathione peroxidase and γ GCS, especially involvement of signal transduction.
6)Nitric oxide inactivates glutathione peroxidase and the accumulated hydrogen peroxide induces Heparin-binding EGF-like growth factor(HB-EGF) as an autocrine protective factor in rat aortic smooth muscle cells via the JNK pathway.

  • Research Products

    (36 results)

All 2001 2000 1999 1998

All Journal Article (32 results) Book (4 results)

  • [Journal Article] Augmented expression of peroxiredoxinVI in rat lung and kidney after birth implies an antioxidative role.2001

    • Author(s)
      Fujii, T. et al.
    • Journal Title

      Eur. J. Biochem. 268

      Pages: 218-224

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Inactivation of glutathione peroxides by NO leads to the accumulation of H2O2 and the induction of HB-EGF via c-Jun NH2-terminal kinase in rat aortic smooth muscle cells.2001

    • Author(s)
      Koh, YH. et al.
    • Journal Title

      FASEB J. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Augmented expression of peroxiredoxin VI in rat lung and kidney after birth implies an antioxidative role.2001

    • Author(s)
      Fujii, T. et al.
    • Journal Title

      Eur.J.Biochem. 268

      Pages: 218-224

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Inactivation of glutathione peroxidase by NO leads to the accumulation of H2O2 and the induction of HB-EGF via c-Jun NH2-terminal kinase in rat aortic smooth muscle cells.2001

    • Author(s)
      Koh, YH. et al.
    • Journal Title

      FASEB J. (i press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Perodoxin IV is a secretable protein with heparin-binding properties under reduced conditions.2000

    • Author(s)
      Okado-Matsumoto, A. et al.
    • Journal Title

      J.Biochem. 127

      Pages: 493-501

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Redox capacity of cells affects inactivation of glutathione reductase by nitrosative stress.2000

    • Author(s)
      Fujii, T. et al.
    • Journal Title

      Archives of Biochemistry and Biophysics 378

      Pages: 123-130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regular training modulates the accumulation of reactive carbonyl derivatices in mitochondrial and cytosolic fractions of rat skeletal muscle.2000

    • Author(s)
      Radak, Z. et al.
    • Journal Title

      Arch Biochem Biophys. 383

      Pages: 114-118

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Peroxiredoxin IV is a secretable protein with heparin-binding properties under reduced conditions.2000

    • Author(s)
      Okado-Matsumoto, A. et al.
    • Journal Title

      J.Biochem. 127

      Pages: 493-501

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regular training modulates the accumulation of reactive carbonyl derivatives in mitochondnal and cytosolic fractions of rat skeletal muscle.2000

    • Author(s)
      Radak, Z. et al.
    • Journal Title

      Arch.Biochem.Biophys. 383

      Pages: 114-118

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional expression of rat thioredoxin reducatase : selenocy insertion sequence element is essential for the active enzyme.1999

    • Author(s)
      Fujiwara, N. et al.
    • Journal Title

      Biochem. J. 340

      Pages: 439-444

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glutathione Peroxidase-like activity of careuloplasmin as an important lung antioxidant.1999

    • Author(s)
      Park, Y.S.et al.
    • Journal Title

      FEBS Lett. 458

      Pages: 133-136

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Co-expression of gamma-glutamylcysteine synthetase sub-units in response to cisplatin doxorubicin in human cancer cells.1999

    • Author(s)
      Iida, T. et al.
    • Journal Title

      Int J Cancer 82

      Pages: 405-411

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Significance of glutathione-dependent antioxidant system in diabetes-induced embryonic malformations.1999

    • Author(s)
      Sakamai, H. et al.
    • Journal Title

      Diabetes. 48

      Pages: 1138-1144

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The molecular basisi of a case of gamma-glutamylcysteine synthetase deficiency.1999

    • Author(s)
      Beutler, E. et al.
    • Journal Title

      Blood. 94

      Pages: 2890-2894

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Calnexin discriminates between protein conformational states and functions as a molecular chaperone in vitro.1999

    • Author(s)
      Ihara, Y. et al.
    • Journal Title

      Mol Cell. 4

      Pages: 331-341

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Superoxide dismutase gene expression in skeletal muscle : fiber-specific adaptation to endurance training.1999

    • Author(s)
      Hollander, J. et al.
    • Journal Title

      Am J Physiol. 277(3P+2)

      Pages: R856-R862

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional expression of rat thioredoxin reductase : selenocy insertion sequence element is essential for the active enzyme.1999

    • Author(s)
      Fujiwara, N. et al.
    • Journal Title

      Biochem.J. 340

      Pages: 439-444

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glutathione Peroxidase-like activity of caeruloplasmin as an important lung antioxidant.1999

    • Author(s)
      Park, Y.S.et al.
    • Journal Title

      FEBS Lett. 458

      Pages: 133-136

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Co-expression of gamma-glutamylcysteine synthetase sub-units in response to cisplatin and doxorubicin in human cancer cells.1999

    • Author(s)
      Iida, T. et al.
    • Journal Title

      Int.J.Cancer 82

      Pages: 405-411

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Significance of glutathione-dependent antioxidant system in diabetes-induced embryonic malformations.1999

    • Author(s)
      Sakamaki, H. et al.
    • Journal Title

      Diabetes 48

      Pages: 1138-1144

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The molecular basis of a case of gamma-glutamylcysteine synthetase deficiency.1999

    • Author(s)
      Beutler, E. et al.
    • Journal Title

      Blood 94

      Pages: 2890-2894

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Calnexin discriminates between protein conformational states and functions as a molecular chaperone in vitro.1999

    • Author(s)
      Ihara, Y. et al.
    • Journal Title

      Mol.Cell 4

      Pages: 331-341

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Superoxide dismutase gene expression in skeletal muscle : fiber-specific adaptation to endurance training.1999

    • Author(s)
      Hollander, J. et al.
    • Journal Title

      Am.J.Physiol. 277(3Pt2)

      Pages: R856-R862

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A defect in the mitochondrial import of mutant Min-superoxide dismutase produced in Sf21 cells.1998

    • Author(s)
      Fujii, J. et al.
    • Journal Title

      J.Biochem. 124

      Pages: 340-346

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glycoxidation in aortic collagen from ATZ-induced diabetic rats and its relevance to vascular dasmage.1998

    • Author(s)
      Meng, J. et al.
    • Journal Title

      Atherosclerosis 136

      Pages: 355-365

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Overexpression of aldehyde reductase protects PC12 cells from the cytotoxicity of methylglyoxal or 3-deoxyglucosone.1998

    • Author(s)
      Suzuki, K. et al.
    • Journal Title

      J.Biochem. 123

      Pages: 354-358

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An actice efflux system for heavy metals in cisplatin-resisitant human KB carcinoma cells.1998

    • Author(s)
      Chen, ZS. et al.
    • Journal Title

      Exp Cell Res. 240

      Pages: 312-320

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tissue ditribution of immunoreactive mouse extracellular superoxide dismutase.1998

    • Author(s)
      Ookawara, T. et al.
    • Journal Title

      Am J Physiol. 175(3p+1)

      Pages: C840-C847

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A defect in the mitochondrial import of mutant Mn-superoxide dismutase produced in Sf21 cells.1998

    • Author(s)
      Fujii, J. et al.
    • Journal Title

      J.Biochem. 124

      Pages: 340-346

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glycoxidation in aortic collagen from ATZ-incuced diabetic rats and its relevance to vascular dasmage.1998

    • Author(s)
      Meng, J. et al.
    • Journal Title

      Atherosclerosis 136

      Pages: 355-365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An active efflux system for heavy metals in cisplatin-resistant human KB carcinoma cells.1998

    • Author(s)
      Chen, ZS. et al.
    • Journal Title

      Exp.Cell Res. 240

      Pages: 312-320

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tissue distribution of immunoreactive mouse extracellular superoxide dismutase.1998

    • Author(s)
      Ookawara, T. et al.
    • Journal Title

      Am.J.Physiol. 275(3Pt1)

      Pages: C840-C847

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Experimetal protocols for reactive oxygen and nitrogen species.2000

    • Author(s)
      Taniguchi, N., Gutteridge, J.M.C.
    • Total Pages
      337
    • Publisher
      Oxford Univ. Press
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Superoxide disumutates in exercise and disease. In : Handbook of Oxidants and Antioxidants in Exercise.2000

    • Author(s)
      Suzuki, K. et al.
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Experimental protocols for reactive oxygen and nitrogen species.2000

    • Author(s)
      Taniguchi, N., Gutteridge, J.M.C.eds.
    • Total Pages
      337
    • Publisher
      Oxford Univ.Press
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Superoxide dismutases in exercise and disease. Handbook of Oxidants and Antioxidants in Exercise.2000

    • Author(s)
      Suzuki, K. et al.
    • Total Pages
      243-298
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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