2000 Fiscal Year Final Research Report Summary
APPROACHES FOR CONTROLLING INFECTION BY GENE THERAPY
| Project/Area Number |
10044299
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | EHIME UNIVERSITY |
|---|
Principal Investigator |
ASANO Yoshihiro EHIME UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (70114353)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUBO Shuichi TOKYO METROPOLITAN MEDICAL RESEARCH INSTITUTE RESEARCH FELLOW, 研究員 (00251223)
NAKAYAMA Toshinori CHIBA UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50237468)
SHINOMIYA Hiroto EHIME UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (80162618)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Keywords | infection immunity / innate immunity / T cell subset / IL-12 p40 / macrophage function |
|---|
| Research Abstract |
Successful development of Th2 cells requires both differentiation and expansion processes, and depends on the activation levels of IL-4 receptor (IL-4R) -mediated signaling. Among signaling molecules downstream of IL-4R, STAT6 activation is thought to be critical for differentiation process, and phosphorylation of IRS-2 is important for proliferation. The activation of Ras/MAPK cascade appears to act on Jak1 kinase to enhance its kinase activity and improve IL-4R function. The activation of CN in naive CD4 T cells induces transcriptional upregulation of Jak3. STAT5 is found to be physically and functionally associated with IL-4 receptor complex in the anti-TCR-activated naive T cells and established cloned Th2 cells. The IL-4-induced activation of STAT5 appears to play an important role in the expansion process of the developing Th2 cells. Thus, the recruitment of Jak3 and STAT5 molecules to functional IL-4R complex in developing Th2 cells appears to be crucial for Th2 cell development.
We demonstrated that the splenomegaly associated with Salmonella-infection, a host-defensive response, was caused by the migration of Mac-1^+ cells into the infected spleen. We tried to generate murine bone marrow-derived DC lines by using a helper-free and replication-defective recombinant retrovirus encoding the SV40 early antigens, and obtained several DC lines that can cytokine-independently grow.
We established the mutant Listeria monocytogenes which expressed ply118 gene product when virulence genes are activated. We tried to establish the gene transduction system using this mutant Listeria.
|
-
-
-
-
-
[Publications] Tan, R.S-P., A.U.Kara, C.Feng, and Y.Asano: "Nitoric oxide is not essential in the protection of Plasmodium berghei blood stage murine malaria infection in IRF-1 (interferon regulatory factor-1) deficient mice."9th International Congress of Parasitology, eds., Tada, I., Kojima, S., and Tsuji, M., Monduzzi Editore, Italy. 1003-1007 (1998)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Publications] Shimoda, K., Kato, K., Aoki, K., Matsuda, T., Miyamoto, A., Shibamori, M., Yamashita, M., Numata, A., Takase, K., Kobayashi, S., Shibata, S., Asano, Y., Gondo, H., Sekiguchi, K., Nakayama, K., Nakayama, T., Okamura, T., Okamura, S., Niho, Y., and Nakayama, K.: "Tyk2 plays a restricted role in IFNγ signaling although it is required for IL-12-mediated T cell function."Immunity. 13. 561-571 (2000)
Description
「研究成果報告書概要(欧文)」より
