1999 Fiscal Year Final Research Report Summary
Generation of bioactive peptides which suppress HIV infection
| Project/Area Number |
10044310
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Nagoya City University |
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Principal Investigator |
OKADA Hidechika Nagoya City Univ. Sch. Med. Prof., 医学部, 教授 (30160683)
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| Co-Investigator(Kenkyū-buntansha) |
WILLIAM Campbell Choju Med. Inst., Res. Fellow, 長寿医学研究所, 主任研究員
OKADA Noriko Nagoya City Univ. Sch. Med. Assoc. Prof., 医学部, 助教授 (20160682)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Protein / Amino acid sequence / Antisense peptide / Antisense homology box / HIV-1 / T20 / gp160 / Electromagnetic resonance |
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| Research Abstract |
Recently we identified a novel motif in proteins that consists of short amphiphilic regions of 6-20 amino acids expressing strong sense-antisense relationships at the protein level. These regions, termed antisense homology boxes (AHB) are separated by approximately 50 amino acid long regions. It is expected that AHBs may be involved in folding, chaperoning and oligomer formation of proteins. AHB-derived peptides have been shown to possess some biological functions with respect to the endothelin receptor and C5a receptor. We detected AHB regions between gp160 envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) and T20, which was a peptide inhibitor of gp41-mediated virus entry, using the "ANTIS" computer program and synthesized peptides. Antisense homology box-derived peptide of gp160, termed T20ASP-1L (amino acids 84-97), shoed inhibitory activity to HIV-1IIIB infection of MT-4 cells. T20ASP-1L site, which exists in gp160 C1 region, was highly homologous each strain of HIV-1 at least 70% over. And T20ASP-1L also inhibited HIV-1MN infection of MT-4 cells weakly. The inhibition of HIV-1 infection by T20ASP-1L (amino acids 84-97) of the gp160 subunit suggests that T20ASP-1L site in C1 region of gp160 may influence something of HIV-1 infection to target cells and biologically active inhibitory peptides can be found by searching for antisense motifs in the target protein.
We also analyzed gp160 with the electromagnetic resonance theory developed by Dr. Irena Cosic and found a specific frequency at T20 region of gp41.
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Research Products
(12 results)
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[Publications] Mizuno, M., Nishikawa, K., Okada, N., Matsuo, S., Ito, K. & Okada, H.: "Inhibition of a membrane complement regulatory protein by a monoclonal antibody induces acute lethal shock in rats primed with LPS."J. Immunol.. 162. 5477-5482 (1999)
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[Publications] Baranyi, L., Campbell, W., Ohshima, K.,, Fujimoto, S., Boros, M., Kaszaki, J. & Okada, H.: "Antisense homology box derived peptides represent a new class of endothelin receptor inhibitors."Peptides. 19. 211-223 (1998)
Description
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