1998 Fiscal Year Annual Research Report

減数分裂関連遺伝子出芽酵母SEP1のマウスホモログDhm2の機能と発現制御

Research Project

Project/Area Number	10160204
Research Institution	The University of Tokyo
Principal Investigator	池田日出男東京大学, 医科学研究所, 教授 (10012775)
Co-Investigator(Kenkyū-buntansha)	菖蒲池健夫東京大学, 医科学研究所, 学振特別研究員
Keywords	SEP1遺伝子 / 減数分裂 / Dhm2遺伝子 / 配偶子形成 / コヘシーン / Rhc21遺伝子 / Rad21遺伝子 / 胞子形成
Research Abstract	出芽酵母のSEP1遺伝子は減数分裂に必須であり、細胞の老化やテロメア長の維持にも関与する。従って、SEP1のマウスホモログであるDhm2は、マウスの配偶子形成、老化あるいは寿命に関連した機能をもつ可能性がある。Dhm2遺伝子の転写産物は非常に特徴的であり、精巣においてORFから予想される大きさの転写産物が検出されるが、精巣以外の臓器では、それよりも大きな転写産物が検出される。この精巣特異的な発現制御のメカニズムを探るため、心臓由来のDhm2cDNAをクローニングした。これを既知の精巣由来のDhm2cDNAと比較したところ、3′非翻訳領域のみが異なっており、この領域がDhm2mRNAの安定性に関与していることが示唆された。FISH法による染色体マッピングの結果、Dhm2遺伝子は第9染色体のF1領域に存在することが分かった。該領域にマップされるdu、tipは、発育不良、短命、不妊といった表現型を示す変異であることから、Dhm2遺伝子との関連が示唆された。一方、出芽酵母RHC21/SCC1/MCD1遺伝子がコードするcohesinは、S期に複製した姉妹染色分体どうしをM期中期までつなぎ止めておくことで未成熟な染色体分配を抑制し、正常なmitosis進行に寄与する蛋白質である。cohesinの減数分裂過程における機能を、rhc21変異体を用いて検討した。その結果、rhc21変異体では、胞子形成率の低下、減数分裂期(および体細胞分裂期)の相同的組換え頻度の低下、がそれぞれみられた。このことから、RHC21が体細胞分裂期の染色体分配に加えて、減数分裂の進行や体細胞分裂期の相同的組換えにも重要な機能を持つことが示された。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Heo,S.-J.,Tatebayashi,K.,Kato,J.,and Ikeda,H.: "Budding yeast RHC21 gene,a homologue of flssion yeast rad21^+ gene,is essential for chromosome segregation." Mol.Gen.Genet.257. 149-156 (1998)
[Publications] Yamagata,K.,Kato,J.,Shimamoto,A.,Goto,M.,Furuichi,Y.,and Ikeda,H.: "Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant Implication for genomic instability in human diseases." Proc.Natl.Acad.Scl.USA. 95. 8733-8738 (1998)
[Publications] Sato,Y.,Shimamoto,A.,Shobuike,T.,Sugimoto,M.,Ikeda,H.,Kuroda,S.,and Furiichi,Y.: "Cloning and characterization of human Sep1(hSEP1)gene and cytoplasmic localization of its product." DNA Res.5. 241-246 (1998)
[Publications] Shanado,Y.,Kato,J.and Ikeda,H.: "Escherichia coli HU protein suppresses DNA-gyrase-mediated illegitimate recombination and SOS induction." Genes Cells. 3. 511-520 (1988)
[Publications] Tsukamoto,Y.and Ikeda,H.: "Double-strand break repair mediated by DNA end-joining." Genes Cells. 3. 135-144 (1998)
[Publications] Onda,M.,Hanada,K.,Kawachi,H.and Ikeda,H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress." Genetics. 151. 439-446 (1999)
[Publications] Tatebayashi,K.,Kato,J.,and Ikeda,H.: "Isolation of a Schizosaccharomyces pom be rad21ts muatnt which is aberrant in chromosome segregation,microtubule function,DNA repair and DNA replication:possible involvement of Rad21 in ubiquitin-mediated proteolysis" Genetics. 148. 49-57 (1998)
[Publications] A.Hasebe,S.Tsushima and S.Iida: "Isolation and characterization of IS1416 from Pseudomonas glumae,an new member of the IS3 family" Plasmid. 39. 196-204 (1998)
[Publications] S.Iida,: "1998 Accessory genes in the darA operon of bacteriophage P1 affect anti-restriction function,generallzed transduction,head morphogenesis and host cell lysis" Virology. 251. 49-58 (1998)
[Publications] Y.Habu,Y.Hisatomi and S.Iida: "Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory." Plant J.16. 371-376 (1998)
[Publications] Y.Inagaki,: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories" Gene. (In press).
[Publications] S.Tanaka,: "Capturing of a genomic HMG domain sequence by an En/Spm related transposable element Tpn 1 in the Japanese morning glory" Mol.Gen.Genet.(In press).

1998 Fiscal Year Annual Research Report

減数分裂関連遺伝子出芽酵母SEP1のマウスホモログDhm2の機能と発現制御

Principal Investigator

池田 日出男 東京大学, 医科学研究所, 教授 (10012775)

Research Products

[Publications] Heo,S.-J.,Tatebayashi,K.,Kato,J.,and Ikeda,H.: "Budding yeast RHC21 gene,a homologue of flssion yeast rad21^+ gene,is essential for chromosome segregation." Mol.Gen.Genet.257. 149-156 (1998)

[Publications] Yamagata,K.,Kato,J.,Shimamoto,A.,Goto,M.,Furuichi,Y.,and Ikeda,H.: "Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant Implication for genomic instability in human diseases." Proc.Natl.Acad.Scl.USA. 95. 8733-8738 (1998)

[Publications] Sato,Y.,Shimamoto,A.,Shobuike,T.,Sugimoto,M.,Ikeda,H.,Kuroda,S.,and Furiichi,Y.: "Cloning and characterization of human Sep1(hSEP1)gene and cytoplasmic localization of its product." DNA Res.5. 241-246 (1998)

[Publications] Shanado,Y.,Kato,J.and Ikeda,H.: "Escherichia coli HU protein suppresses DNA-gyrase-mediated illegitimate recombination and SOS induction." Genes Cells. 3. 511-520 (1988)

[Publications] Tsukamoto,Y.and Ikeda,H.: "Double-strand break repair mediated by DNA end-joining." Genes Cells. 3. 135-144 (1998)

[Publications] Onda,M.,Hanada,K.,Kawachi,H.and Ikeda,H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress." Genetics. 151. 439-446 (1999)

[Publications] Tatebayashi,K.,Kato,J.,and Ikeda,H.: "Isolation of a Schizosaccharomyces pom be rad21ts muatnt which is aberrant in chromosome segregation,microtubule function,DNA repair and DNA replication:possible involvement of Rad21 in ubiquitin-mediated proteolysis" Genetics. 148. 49-57 (1998)

[Publications] A.Hasebe,S.Tsushima and S.Iida: "Isolation and characterization of IS1416 from Pseudomonas glumae,an new member of the IS3 family" Plasmid. 39. 196-204 (1998)

[Publications] S.Iida,: "1998 Accessory genes in the darA operon of bacteriophage P1 affect anti-restriction function,generallzed transduction,head morphogenesis and host cell lysis" Virology. 251. 49-58 (1998)

[Publications] Y.Habu,Y.Hisatomi and S.Iida: "Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory." Plant J.16. 371-376 (1998)

[Publications] Y.Inagaki,: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories" Gene. (In press).

[Publications] S.Tanaka,: "Capturing of a genomic HMG domain sequence by an En/Spm related transposable element Tpn 1 in the Japanese morning glory" Mol.Gen.Genet.(In press).

池田日出男東京大学, 医科学研究所, 教授 (10012775)