1999 Fiscal Year Annual Research Report

糖鎖リモデリングと細胞コミュニケーション

Research Project

Project/Area Number	10178105
Research Institution	Osaka University
Principal Investigator	谷口直之大阪大学, 医学系研究科, 教授 (90002188)
Co-Investigator(Kenkyū-buntansha)	鈴木明身理化学研究所, フロンティア研究システム, グループリーダー (70134533) 斎藤政樹国立がんセンター研究所, 部長 (60012762) 村松喬名古屋大学, 医学部, 教授 (00030891) 川嵜敏祐京都大学, 大学院・薬学研究科, 教授 (50025706) 小川智也理化学研究所, 理事 (30087572)
Keywords	糖鎖 / 複合糖質 / 糖鎖リモデリング / 糖鎖遺伝子 / 糖タンパク質 / 糖脂質 / プロテオグリカン / 糖転移酵素
Research Abstract	糖鎖は糖タンパク質、糖脂質、プロテオグリカンなどの重要な構成成分であり、生体内で多種多様な分子群を形成している。この分野を包括する糖鎖生物学はポストゲノム研究の中心的課題の一つと考えられ、本研究領域の果たす役割は益々大きくなってきている。本研究領域は二つの計画研究班、谷口を班長とする第1班「糖鎖遺伝子による細胞リモデリング」と村松を班長とする第2班「糖鎖シグナルによる細胞間コミュニケーションの制御」からなり、これらに属する計画研究班班員および公募の班員間相互の交流、連携の促進、研究成果のとりまとめと刊行などをはかるため領域代表者を班長とする総括班をおいている。本年度はニュースレターの刊行、合同班会議、公開シンポジウムの開催などをおこなったが、とくに公開シンポジウムでは海外よりこの分野における第1人者3名を招き、これまで以上に活発な議論が行われ、各班員ともそれぞれの課題について非常に有意義な示唆をえることができた。また、本年度は国際糖鎖生物学会議(GlycoXV)が東京で開催され、総括班の班員も中心的な役割を担ったが、本研究領域の各班員と来日した多数の海外研究者との共同研究を含む研究交流や討論を促す機会もえることができた。このような活動を通して、班員間が相互に補強しあうような環境を提供し、各課題をより効率的にすすめられるよう配慮した。実際に班員間の共同研究によって大きな進展のみられた課題も多数あり、ゴードンカンファレンスや国際糖鎖生物学会議、国際糖質化学シンポジウムでも高い評価を受けている。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] E. Miyoshi et al. and N. Taniguchi: "Overexpression of α1-6 fucosyltransferase in hepatoma cells suppresses intrahepatic metastasis after splenic injection in athymic mice"Cancer Res.. 59. 2237-2243 (1999)
[Publications] J. -H. Ko et al. and N. Taniguchi: "Regulation of the GnT-V promoter by transcription factor Ets-1 in various cancer cell lines"J. Biol. Chem.. 274. 22941-22948 (1999)
[Publications] S. Hitoshi et al. and S. Tsuji: "Dorsal root ganglia neuron-specific promoter activity of the rabbit beta-galactoside alpha1, 2-fucosyltransferase gene"J. Biol. Chem.. 274. 389-396 (1999)
[Publications] Y. -C. Lee, et al. and S. Tsuji: "Molecular cloning and functional expression of two members of mouse NeuAcα2, 3 Gal β1, 3GalNAc GalNAc α2, 6-sialyltransferase family, ST6GalNAc III and IV"J. Biol. Chem.. 274. 11958-11967 (1999)
[Publications] H. Liu et al. and S. Tsuji: "Isolation of 10 differentially expressed cDNAs in differentiated Neuro2a cells induced through controlled expression of the GD3 synthase gene"J. Neurochem.. 72. 1781-1790 (1999)
[Publications] S. Takasuga, T. Katada, M. Ui and O. Hazaki: "Enhancement by adenosine of insulin-induced activation of phosphoinositide 3-kinase and protein kinase B in rat adipocytes"J. Biol. Chem.. 274. 19545-19550 (1999)
[Publications] J. Kon et al., M. Ui and F. Okajima: "Comparison of intrinsic activities of the putative sphingosine 1-phosphate receptor subtypes to regulate several signaling pathways in their cDNA-transfected Chinese hamster ovary cells"J. Biol. Chem.. 274. 23940-23947 (1999)
[Publications] T. Suzuki et al., T. Nagatsu and H. Ichinose: "Characterization of wild-type and mutants of recombinant human GTP cyclohydrolase I : Relationship to etiology of dopa-responsive dystonia"J. Neurochem.. 73. 2510-2516 (1999)
[Publications] M. Sawada et al. and T. Nagatsu T.: "Interleukin-10 inhibits both production of cytokines and expression of cytokine receptors in microglia"J. Neurochem.. 72. 1466-1471 (1999)
[Publications] N. Kimura, et al., T. Muramatsu, and R. Kannagi: "Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by co-transfection of α1, 3 fucosyltransferase VII and newly cloned GlcNAcβ : 6-sulfotransferase cDNA"Proc. Natl. Acad. Sci. USA. 96. 4530-4535 (1999)
[Publications] N. Maeda et al., T. Muramatsu and M. Noda: "A receptor-like protein tyrosine phosphatase (PTPζ/RPTPβ) binds a heparin binding growth factor midkine : Involvement of arginine 78 of midkine in the high affinity binding to PTPζ"J. Biol. Chem.. 274. 12474-12479 (1999)
[Publications] K. Owada et al., T. Muramatsu and M. Michikawa: "Midkine inhibits caspase-dependent apoptosis via the activation of mitogen-activated protein kinase and phosphatidyl-inositol 3-kinase in cultured neurons"J. Neurochem.. 73. 2084-2092 (1999)
[Publications] K. Ishiguro et al. and T. Muramatsu: "Syndecan-4 deficiency impairs focal adhesion formation only under restricted conditions"J. Biol. Chem.. 275. 5249-5252 (2000)
[Publications] M. Horiba et al. and T. Muramatsu: "Neointima formation in a restenosis model is suppressed in midkine-dificient mice"J. Clin. Invest.. 105. 489-495 (2000)
[Publications] Y. Ma et al. and T. Kawasaki: "Antitumor activity of mannan-binding protein in vivo as revealed by a virus expression system : mannan-binding proteindependent cell-mediated cytotoxicity"Proc. Natl. Acad. Sci. USA.. 96. 371-375 (1999)

1999 Fiscal Year Annual Research Report

糖鎖リモデリングと細胞コミュニケーション

Principal Investigator

谷口 直之 大阪大学, 医学系研究科, 教授 (90002188)

Research Products

[Publications] E. Miyoshi et al. and N. Taniguchi: "Overexpression of α1-6 fucosyltransferase in hepatoma cells suppresses intrahepatic metastasis after splenic injection in athymic mice"Cancer Res.. 59. 2237-2243 (1999)

[Publications] J. -H. Ko et al. and N. Taniguchi: "Regulation of the GnT-V promoter by transcription factor Ets-1 in various cancer cell lines"J. Biol. Chem.. 274. 22941-22948 (1999)

[Publications] S. Hitoshi et al. and S. Tsuji: "Dorsal root ganglia neuron-specific promoter activity of the rabbit beta-galactoside alpha1, 2-fucosyltransferase gene"J. Biol. Chem.. 274. 389-396 (1999)

[Publications] Y. -C. Lee, et al. and S. Tsuji: "Molecular cloning and functional expression of two members of mouse NeuAcα2, 3 Gal β1, 3GalNAc GalNAc α2, 6-sialyltransferase family, ST6GalNAc III and IV"J. Biol. Chem.. 274. 11958-11967 (1999)

[Publications] H. Liu et al. and S. Tsuji: "Isolation of 10 differentially expressed cDNAs in differentiated Neuro2a cells induced through controlled expression of the GD3 synthase gene"J. Neurochem.. 72. 1781-1790 (1999)

[Publications] S. Takasuga, T. Katada, M. Ui and O. Hazaki: "Enhancement by adenosine of insulin-induced activation of phosphoinositide 3-kinase and protein kinase B in rat adipocytes"J. Biol. Chem.. 274. 19545-19550 (1999)

[Publications] J. Kon et al., M. Ui and F. Okajima: "Comparison of intrinsic activities of the putative sphingosine 1-phosphate receptor subtypes to regulate several signaling pathways in their cDNA-transfected Chinese hamster ovary cells"J. Biol. Chem.. 274. 23940-23947 (1999)

[Publications] T. Suzuki et al., T. Nagatsu and H. Ichinose: "Characterization of wild-type and mutants of recombinant human GTP cyclohydrolase I : Relationship to etiology of dopa-responsive dystonia"J. Neurochem.. 73. 2510-2516 (1999)

[Publications] M. Sawada et al. and T. Nagatsu T.: "Interleukin-10 inhibits both production of cytokines and expression of cytokine receptors in microglia"J. Neurochem.. 72. 1466-1471 (1999)

[Publications] N. Maeda et al., T. Muramatsu and M. Noda: "A receptor-like protein tyrosine phosphatase (PTPζ/RPTPβ) binds a heparin binding growth factor midkine : Involvement of arginine 78 of midkine in the high affinity binding to PTPζ"J. Biol. Chem.. 274. 12474-12479 (1999)

[Publications] K. Owada et al., T. Muramatsu and M. Michikawa: "Midkine inhibits caspase-dependent apoptosis via the activation of mitogen-activated protein kinase and phosphatidyl-inositol 3-kinase in cultured neurons"J. Neurochem.. 73. 2084-2092 (1999)

[Publications] K. Ishiguro et al. and T. Muramatsu: "Syndecan-4 deficiency impairs focal adhesion formation only under restricted conditions"J. Biol. Chem.. 275. 5249-5252 (2000)

[Publications] M. Horiba et al. and T. Muramatsu: "Neointima formation in a restenosis model is suppressed in midkine-dificient mice"J. Clin. Invest.. 105. 489-495 (2000)

[Publications] Y. Ma et al. and T. Kawasaki: "Antitumor activity of mannan-binding protein in vivo as revealed by a virus expression system : mannan-binding proteindependent cell-mediated cytotoxicity"Proc. Natl. Acad. Sci. USA.. 96. 371-375 (1999)

谷口直之大阪大学, 医学系研究科, 教授 (90002188)