2000 Fiscal Year Annual Research Report

糖鎖リモデリングと細胞コミュニケーション

Research Project

Project/Area Number	10178105
Research Institution	Osaka University
Principal Investigator	谷口直之大阪大学, 医学系研究科, 教授 (90002188)
Co-Investigator(Kenkyū-buntansha)	鈴木明身理化学研究所, フロンティア研究システム, グループリーダー (70134533) 斎藤政樹国立がんセンター研究所, 部長 (60012762) 村松喬名古屋大学, 医学研究科, 教授 (00030891) 川嵜敏祐京都大学, 薬学研究科, 教授 (50025706) 小川智也理化学研究所, 理事 (30087572)
Keywords	糖鎖 / 複合糖質 / 糖鎖リモデリング / 糖鎖遺伝子 / 糖タンパク質 / 糖脂質 / プロテオグリカン / 糖転移酵素
Research Abstract	本研究領域総括班は、合同班会議やニュースレターの刊行を通して、二つの計画研究班、第1班「糖鎖遺伝子による細胞リモデリング」および第2班「糖鎖シグナルによる細胞間コミュニケーションの制御」に属する計画班班員と公募の班員の共同研究といった連携を促進させ、また公開シンポジウムを通して、本研究領域に関係する多くの共同研究者間の相互交流も促した。本研究領域では多くの優れた成果を得ることが出来、わが国の糖鎖生物学分野に大きな進展をもたらすことができたと考えている。糖鎖は、複合糖質と呼ばれる糖タンパク質、糖脂質、プロテオグリカンといった一群の生体物質の主要な構成成分であり、これらの複合糖質の生理機能に、糖鎖は不可欠な役割を担っている。例えば糖タンパク質の場合、遺伝子から翻訳されたポリペプチドのみでは機能をもたず、糖鎖が付加してはじめて最終的な機能分子が形成されることからもわかるように、ゲノム研究だけでは生命現象を十分に理解することはきわめて難しく、糖鎖をはじめとする翻訳後修飾は大変重要な位置を占めることが認識されつつある。その中でも糖鎖は構造の多様性や存在の普遍性という点で重要性が非常に高く、ポストゲノム研究の中心的課題であることは間違いない。わが国におけるこの分野の研究をさらに展開させるうえで、今年度で終了する本研究領域の果たした役割は大きいといえる。

Research Products
(17 results)

All Other

All Publications (17 results)

[Publications] Sato Y,Takahashi M,Shibukawa Y,Jain SK,Hamaoka R,Miyagawa Ji,Yaginuma Y,Honke K,Ishikawa M,Taniguchi N: "Overexpression of N-acetylglucosaminyltransferase III enhances the EGF-induced phosphorylation of ERK in HeLaS3 cells by upregulation of the internalization rate of the receptors."J.Biol.Chem.. (in press).
[Publications] Honke K,Tsuda M,Koyota S,Wada Y,Iida-Tanaka N,Ishizuka I,Nakayama J,Taniguchi N: "Molecular Cloning and Characterization of a Human beta-Gal-3'-sulfotransferase That Acts on Both Type1 and Type2 (Galbeta 1-3/1-4GlcNAc-R) Oligosaccharides"J.Biol.Chem.. 276. 267-274 (2001)
[Publications] Kitada T,Miyoshi E,Noda K,Higashiyama S,Ihara H,Matsuura N,Hayashi N,Kawata S,Matsuzawa Y,Taniguchi N: "The Addition of Bisecting N-Acetylglucosamine Residues to E-cadherin Down-regulates the Tyrosine phosphorylation of beta-Catenin"J.Biol.Chem.. 276. 475-480 (2001)
[Publications] Sasai K,Ikeda Y,Tsuda T,Ihara H,Korekane H,Shiota K,Taniguchi N: "The Critical Role of the Stem Region as a Functional Domain Responsible for the Oligomerization and Golgi Localization of N-Acetylglucosaminyltransferase V.THE IN VOLVEMENT OF A DOMAIN HOMOPHILICINTERACTION"J.Biol.Chem.. 276. 759-765 (2001)
[Publications] Sakamoto Y,Taguchi T,Honke K,Korekane H,Watanabe H,Tano Y,Dohmae N,Takio K,Horii A,Taniguchi N: "Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc) : GlcNAcbeta 1-6(GlcNAcbeta 1-2)-manalpha 1-R[GlcNAc to man] beta 1,4N-acetylglucosaminyltransferase VI"J.Biol.Chem.. 275. 36029-36034 (2000)
[Publications] Tsuda T,Ikeda Y,Taniguchi N: "The Asn-420-linked sugar chain in human epidermal growth factor receptor suppresses ligand-independent spontaneous oligomerization.Possible role of a specific sugar ehain in controllable receptor activation"J.Biol.Chem.. 275. 21988-21994 (2000)
[Publications] Ueoka C,Kaneda N,Okazaki I,Nadanaka S,Muramatsu T,Sugahara K: "Neuronal cell adhesion,mediated by the heparin-binding neuroregulatory factor midkine, is specifically inhibited by chondroitin sulfate E Structural and functional implications of the over-sulfated chondroitin sulfate"J.Biol.Chem.. 275. 37407-37413 (2000)
[Publications] Ogawa H,Muramatsu H,Kobayashi T,Morozumi K,Yokoyama I,Kurosawa N,Nakao A,Muramatsu T.: "Molecular Cloning of endo-beta-galactosidase C and its application in removing alpha-galactosyl xenoantigen from blood vessels in the pig kidney"J.Biol.Chem.. 275. 19368-19374 (2000)
[Publications] Adachi,Y,Reynolds,PN,Yamamoto,M,Grizzle WE,Overturf,K,Matsubara,S,Muramatsu,T and Curiel,DT: "Midkine promoter-based adenoviral vector gene delivery for pediatric solid tumors"Cancer Res.. 60. 4305-4310 (2000)
[Publications] Ishiguro,K,Kojima,T,Kadomatsu,K,Nakayama,Y,Takagi,A,Suzuki,M,Takeda,N,Ito,M,Yamamoto,K,Matsushita,T,Kusugami,K,Muramatsu,T and Saito,H: "Complete antithrombin deficiency in mice results in embryonic lethality"J.Clin.Invest.. 106. 873-878 (2000)
[Publications] Qi,M.,Ikematsu,S.,Maeda,N.,Ichihara-Tanaka,K.,Sakuma,S.,Noda,M.,Muramatsu,T.and Kadomatsu,K.: "Involvement of protein-tyrosine phosphatasez, mitogen-activated protein kinase and phosphatidylinositol 3-kinase"J.Biol.Chem.. (in press).
[Publications] Nakamura,K.,Kojima,H.,Suzuki,M.,Suzuki,A.and Tamai,Y.: "Novel polysialogangliosides of skate brain : Structural determination of tetra-, penta-, and hexasialogangliosides with NeuAc-GalNAc sequence and neuritogenic activity"Eur.J.Biochem.. 267. 5198-5208 (2000)
[Publications] Kobayashi K. et al.: "Modest neuropsychological deficits caused reduced noradrenaline metabolism in mice heterologous for a mutated tyrosine hydroxylase gene"J.Neurosci.. 20. 2418-2426 (2000)
[Publications] Kaneko S.,Hikida T.,Watanabe D.,Ichinose H.,Nagatsu T.,Kreitman R.J.,Pastan I.,Nakanishi S.: "Synaptic integration mediated by striatal cholinergic interneurons in basal ganglia function."Science. 289. 633-637 (2000)
[Publications] Shimohata T. et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription"Nature Genet.. 26. 29-35 (2000)
[Publications] T.Kanazawa,S.Nakamura,M.Momoi,T.Yamaji,H.Takematsu,H.Yano,H.Sabe,A.Yamamoto,T.Kawasaki,Y.Kozutsumi: "Inhibition of cytokinesis by a lipid metabolite, psychosine"J.Cell Biol.. 149. 943-950 (2000)
[Publications] Y.Sun,R.Taniguchi,D.Tanoue,T.Yamaji,H.Takematsu,K.Mori,T.Fujita,T.Kawasaki,Y.Kozutsumi: "Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast"Mol.Cell Biol.. 20. 4411-4119 (2000)

2000 Fiscal Year Annual Research Report

糖鎖リモデリングと細胞コミュニケーション

Principal Investigator

谷口 直之 大阪大学, 医学系研究科, 教授 (90002188)

Research Products

[Publications] Honke K,Tsuda M,Koyota S,Wada Y,Iida-Tanaka N,Ishizuka I,Nakayama J,Taniguchi N: "Molecular Cloning and Characterization of a Human beta-Gal-3'-sulfotransferase That Acts on Both Type1 and Type2 (Galbeta 1-3/1-4GlcNAc-R) Oligosaccharides"J.Biol.Chem.. 276. 267-274 (2001)

[Publications] Kitada T,Miyoshi E,Noda K,Higashiyama S,Ihara H,Matsuura N,Hayashi N,Kawata S,Matsuzawa Y,Taniguchi N: "The Addition of Bisecting N-Acetylglucosamine Residues to E-cadherin Down-regulates the Tyrosine phosphorylation of beta-Catenin"J.Biol.Chem.. 276. 475-480 (2001)

[Publications] Tsuda T,Ikeda Y,Taniguchi N: "The Asn-420-linked sugar chain in human epidermal growth factor receptor suppresses ligand-independent spontaneous oligomerization.Possible role of a specific sugar ehain in controllable receptor activation"J.Biol.Chem.. 275. 21988-21994 (2000)

[Publications] Ogawa H,Muramatsu H,Kobayashi T,Morozumi K,Yokoyama I,Kurosawa N,Nakao A,Muramatsu T.: "Molecular Cloning of endo-beta-galactosidase C and its application in removing alpha-galactosyl xenoantigen from blood vessels in the pig kidney"J.Biol.Chem.. 275. 19368-19374 (2000)

[Publications] Adachi,Y,Reynolds,PN,Yamamoto,M,Grizzle WE,Overturf,K,Matsubara,S,Muramatsu,T and Curiel,DT: "Midkine promoter-based adenoviral vector gene delivery for pediatric solid tumors"Cancer Res.. 60. 4305-4310 (2000)

[Publications] Ishiguro,K,Kojima,T,Kadomatsu,K,Nakayama,Y,Takagi,A,Suzuki,M,Takeda,N,Ito,M,Yamamoto,K,Matsushita,T,Kusugami,K,Muramatsu,T and Saito,H: "Complete antithrombin deficiency in mice results in embryonic lethality"J.Clin.Invest.. 106. 873-878 (2000)

[Publications] Qi,M.,Ikematsu,S.,Maeda,N.,Ichihara-Tanaka,K.,Sakuma,S.,Noda,M.,Muramatsu,T.and Kadomatsu,K.: "Involvement of protein-tyrosine phosphatasez, mitogen-activated protein kinase and phosphatidylinositol 3-kinase"J.Biol.Chem.. (in press).

[Publications] Nakamura,K.,Kojima,H.,Suzuki,M.,Suzuki,A.and Tamai,Y.: "Novel polysialogangliosides of skate brain : Structural determination of tetra-, penta-, and hexasialogangliosides with NeuAc-GalNAc sequence and neuritogenic activity"Eur.J.Biochem.. 267. 5198-5208 (2000)

[Publications] Kobayashi K. et al.: "Modest neuropsychological deficits caused reduced noradrenaline metabolism in mice heterologous for a mutated tyrosine hydroxylase gene"J.Neurosci.. 20. 2418-2426 (2000)

[Publications] Kaneko S.,Hikida T.,Watanabe D.,Ichinose H.,Nagatsu T.,Kreitman R.J.,Pastan I.,Nakanishi S.: "Synaptic integration mediated by striatal cholinergic interneurons in basal ganglia function."Science. 289. 633-637 (2000)

[Publications] Shimohata T. et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription"Nature Genet.. 26. 29-35 (2000)

[Publications] T.Kanazawa,S.Nakamura,M.Momoi,T.Yamaji,H.Takematsu,H.Yano,H.Sabe,A.Yamamoto,T.Kawasaki,Y.Kozutsumi: "Inhibition of cytokinesis by a lipid metabolite, psychosine"J.Cell Biol.. 149. 943-950 (2000)

[Publications] Y.Sun,R.Taniguchi,D.Tanoue,T.Yamaji,H.Takematsu,K.Mori,T.Fujita,T.Kawasaki,Y.Kozutsumi: "Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast"Mol.Cell Biol.. 20. 4411-4119 (2000)

谷口直之大阪大学, 医学系研究科, 教授 (90002188)