1999 Fiscal Year Annual Research Report

組織・器官分化の分子機構

Research Project

Project/Area Number	10182102
Research Institution	Nagoya University
Principal Investigator	町田泰則名古屋大学, 大学院・理学研究科, 教授 (80175596)
Co-Investigator(Kenkyū-buntansha)	中村研三名古屋大学, 大学院・生命農学研究科, 教授 (80164292) 塚谷裕一岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (90260512) 島本功奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427) 西村いくこ京都大学, 大学院・理学研究科, 教授 (00241232) 西谷和彦東北大学, 大学院・理学研究科, 教授 (60164555)
Keywords	形態形成遺伝子 / シロイヌナズナ / 変異体 / 葉 / メリステム / 光周性 / 糖応答 / 細胞形態
Research Abstract	本計画研究班の目的は、メリステムからの植物器官・組織の発生分化の分子的背景を様々な角度から研究することである。町田は、葉の扁平性と左右対称性について、シロイヌナズナの変異体であるale1とas2を用いて研究した。その結果、前者では表皮細胞の分化が、後者では維管束の分化が異常になっていることがわかった。ale1については遺伝子クローニングを行い、その塩基配列からサチライシンファミリーに属するセリンプロテアーゼをコードしている可能性が示唆された。それに関連してシロイヌナズナからトウモロコシのCRINKLY4(CR4)遺伝子のホモログ(ATCR4)をクローニングし、in situで発現部位を検討した。その結果、ATCR4はALE1遺伝子が発現している細胞の隣の細胞で発現していることがわかった。AS2遺伝子については、独自の方法により染色体歩行を行い、原因遺伝子をクローニングした。さらに、その変異が影響を与えているメリステム関連遺伝子を探索した。島本は、短日植物であるイネの光周性を研究し、光周性をなくした変異体se-5の原因遺伝子がHY1である可能性を示した。塚谷は、葉の長軸方向と横軸方向の成長の制御機構を研究してきた。クローニングしたROT遺伝子の発現系を用いて、これが葉の長軸方向への伸長に関わっていることを示した。中村は、しょ糖に対する植物の応答と器官分化の関連を研究するために、7500系統のアクティベイションタギングライブラリーを作製し、その中から、糖応答が異常になった変異体とメリステムが大きくなる新奇な変異体を得た。西谷は、タバコ培養細胞BY-2にEXGT cDNAアンティセンスを導入し、細胞形態と個体レベルでの異常を研究した。西村は、液胞へのタンパク質輸送系を研究して、小胞(PAC)に存在するタンパク質プロセシング機構を明らかにした。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] Kojima, S.: "A binary vector plasmid for gene expression in plant cells that is stably maintained in Agrobacterium cells"DNA Research. 6. 407-410 (1999)
[Publications] Bhalerao, R: "Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases"Proc. Natl. Acad. Sci. USA. 96. 5322-5327 (1999)
[Publications] Koide, Y.: "The N-terminal propeptide and the C terminus of the precursor to 20-kilo-dalton potato tuber protein can function as different types of vacuolar sorting signals"Plant Cell Physiol.. 40. 1152-1159 (1999)
[Publications] Yang, H.: "Oryza sativa PSK gene encodes a precursor of phytosulfokine-a, a novel sulfated peptide growth factor found in plants"Proc. Natl. Acad. Sci. USA. 96. 13560-13565 (1999)
[Publications] Imanishi, S.: "Aspirin and salicylic acid do not inhibit methyl jasmonate-inducible expression of a gene for ornithine decarboxylasein tobacco BY-2 cells"Biosci. Biotechnol. Biochem.. 64. 125-133 (2000)
[Publications] Maeo, K.: "Expression patterns of two genes for the delta-subunit of mitochondrial F1-ATP synthase from sweet potato transgenic tobacco plants and cells"Plant Cell Physiol.. 40. 866-873 (1999)
[Publications] K. Morino: "Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene"Plant J. 17. 275-285 (1999)
[Publications] A. Takahashi: "Lesion-mimic mutants of rice with alterations in early signaling events of defense"Plant J. 17. 535-545 (1999)
[Publications] K. Shimamoto: "Programmed cell death in plants"Plant Biotechnology. 16. 49-53 (1999)
[Publications] T. Kawasaki: "The small GTP-binding protein Rac is a regulator of cell death in plants"Proc. Natl. Acad. Sci. USA. 96. 10922-10926 (1999)
[Publications] H. Enoki: "Ac as a tool for functional genomics of rice"Plant J. 19. 605-613 (1999)
[Publications] Nanjo, T.: "Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana"Plant J. 18. 185-194 (1999)
[Publications] Kim, G.-T.: "Changes in the Shapes of Leaves and Flowers upon Overexpression of the novel cytochrome P450 in Arabidopsi"Proc. Natl. Acad. Sci., USA. 99. 9433-9437 (1999)
[Publications] Tsukaya, H.: "Molecular mechanisms related ot the evoltion of leaf shape"Proceedings of IIAS International Symposium. (印刷中). (2000)
[Publications] Ito, H.: "Visualization of EXGT-mediated molecular grafting activity in muro by means of a fluorescently labeled xyloglucan oligomer"Plant Cell Physiol.. 40. 1172-1176 (1999)
[Publications] Akamatsu, T.: "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis thaliana"Plant Physiol.. 121. 715-721 (1999)
[Publications] Takano, M.: "Endoxyloglucan transferase gene isolated from seedling roots of ageotropum pea and its fluctuating expression in hydrotropically responding roots"Plant Cell Physiol.. 40. 135-142 (1999)
[Publications] Yamada, K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor by vacuolar processing enzyme"J. Biol. Chem.. 274. 2563-2570 (1999)
[Publications] Machida, C.: "Use of the R-RS site-specific recombination system in plants"Plant Molecular Biology Manual (ed. by Gelvin, S. B.) published by Kluwer Academic Publishers (Netherland). 23 (2000)
[Publications] Nishihama, R.: "The MAP kinase cascade that includes MAPKKK-related protein kinase NPK1 controls a mitotic process in plant cells"Plant MAP kinase cascades (ed. by Heri Hirt) published by Springer-Verlag Heidelberg (Berlin). 12 (1999)
[Publications] K. Shimamoto: "Molecular Biology of Rice"Springer Verlag, Tokyo. 304 (1999)
[Publications] T. Izawa: "Molecular Biology of Rice"Springer Verlag, Tokyo. 17 (1999)
[Publications] Nishitani, K.: "Glycoenzymes"Cross-linking enzymes(1) Endoxyloglucan transferase. 12 (2000)

1999 Fiscal Year Annual Research Report

組織・器官分化の分子機構

Principal Investigator

町田 泰則 名古屋大学, 大学院・理学研究科, 教授 (80175596)

Research Products

[Publications] Kojima, S.: "A binary vector plasmid for gene expression in plant cells that is stably maintained in Agrobacterium cells"DNA Research. 6. 407-410 (1999)

[Publications] Bhalerao, R: "Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases"Proc. Natl. Acad. Sci. USA. 96. 5322-5327 (1999)

[Publications] Koide, Y.: "The N-terminal propeptide and the C terminus of the precursor to 20-kilo-dalton potato tuber protein can function as different types of vacuolar sorting signals"Plant Cell Physiol.. 40. 1152-1159 (1999)

[Publications] Yang, H.: "Oryza sativa PSK gene encodes a precursor of phytosulfokine-a, a novel sulfated peptide growth factor found in plants"Proc. Natl. Acad. Sci. USA. 96. 13560-13565 (1999)

[Publications] Imanishi, S.: "Aspirin and salicylic acid do not inhibit methyl jasmonate-inducible expression of a gene for ornithine decarboxylasein tobacco BY-2 cells"Biosci. Biotechnol. Biochem.. 64. 125-133 (2000)

[Publications] Maeo, K.: "Expression patterns of two genes for the delta-subunit of mitochondrial F1-ATP synthase from sweet potato transgenic tobacco plants and cells"Plant Cell Physiol.. 40. 866-873 (1999)

[Publications] K. Morino: "Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene"Plant J. 17. 275-285 (1999)

[Publications] A. Takahashi: "Lesion-mimic mutants of rice with alterations in early signaling events of defense"Plant J. 17. 535-545 (1999)

[Publications] K. Shimamoto: "Programmed cell death in plants"Plant Biotechnology. 16. 49-53 (1999)

[Publications] T. Kawasaki: "The small GTP-binding protein Rac is a regulator of cell death in plants"Proc. Natl. Acad. Sci. USA. 96. 10922-10926 (1999)

[Publications] H. Enoki: "Ac as a tool for functional genomics of rice"Plant J. 19. 605-613 (1999)

[Publications] Nanjo, T.: "Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana"Plant J. 18. 185-194 (1999)

[Publications] Kim, G.-T.: "Changes in the Shapes of Leaves and Flowers upon Overexpression of the novel cytochrome P450 in Arabidopsi"Proc. Natl. Acad. Sci., USA. 99. 9433-9437 (1999)

[Publications] Tsukaya, H.: "Molecular mechanisms related ot the evoltion of leaf shape"Proceedings of IIAS International Symposium. (印刷中). (2000)

[Publications] Ito, H.: "Visualization of EXGT-mediated molecular grafting activity in muro by means of a fluorescently labeled xyloglucan oligomer"Plant Cell Physiol.. 40. 1172-1176 (1999)

[Publications] Akamatsu, T.: "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis thaliana"Plant Physiol.. 121. 715-721 (1999)

[Publications] Takano, M.: "Endoxyloglucan transferase gene isolated from seedling roots of ageotropum pea and its fluctuating expression in hydrotropically responding roots"Plant Cell Physiol.. 40. 135-142 (1999)

[Publications] Yamada, K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor by vacuolar processing enzyme"J. Biol. Chem.. 274. 2563-2570 (1999)

[Publications] Machida, C.: "Use of the R-RS site-specific recombination system in plants"Plant Molecular Biology Manual (ed. by Gelvin, S. B.) published by Kluwer Academic Publishers (Netherland). 23 (2000)

[Publications] Nishihama, R.: "The MAP kinase cascade that includes MAPKKK-related protein kinase NPK1 controls a mitotic process in plant cells"Plant MAP kinase cascades (ed. by Heri Hirt) published by Springer-Verlag Heidelberg (Berlin). 12 (1999)

[Publications] K. Shimamoto: "Molecular Biology of Rice"Springer Verlag, Tokyo. 304 (1999)

[Publications] T. Izawa: "Molecular Biology of Rice"Springer Verlag, Tokyo. 17 (1999)

[Publications] Nishitani, K.: "Glycoenzymes"Cross-linking enzymes(1) Endoxyloglucan transferase. 12 (2000)

町田泰則名古屋大学, 大学院・理学研究科, 教授 (80175596)