1998 Fiscal Year Annual Research Report

シンクロトロン放射光による生物マシーナリーの構造生物学

Research Project

Project/Area Number	10188101
Research Category	Grant-in-Aid for Scientific Research on Priority Areas (A)
Research Institution	Nagaoka University of Technology
Principal Investigator	三井幸雄長岡技術科学大学, 工学部, 教授 (40012637)
Co-Investigator(Kenkyū-buntansha)	豊島近東京大学, 分子細胞生物学研究所, 教授 (70172210) 田之倉優東京大学, 大学院・農学生命科学研究科, 教授 (60136786) 三木邦夫京都大学, 大学院・理学研究科, 教授 (10116105) 田中勲北海道大学, 大学院・理学研究科, 教授 (70093052) 月原冨武大阪大学, 蛋白質研究所, 教授 (00032277)
Keywords	シンクロトロン放射光 / 生物マシーナリー / 構造生物学 / X線結晶学 / 蛋白結晶学 / 超分子複合体 / 膜蛋白質
Research Abstract	1)研究項目の名称の改訂:A01 膜非結合型の複合体型マシーナリー;A02膜結合型の複合体型マシーナリー;A03集合系型のマシーナリー:A04生物マシーナリーの結晶構造解析のための方法論とした.やや広い定義とし、公募研究班の参加を容易にした、2)世話人会の設置:田中勲(A01)、三木邦夫(A02)、田之倉優(A03)、月原冨武(A04)の4人の世話人を置き、これらと領域代表(三井)の5名で世話人会を設置した.その位置ずけは領域代表の諮問機関である.3)学会における関連シンポジウムの開催:a)蛋白合同年会(長岡98)(平成10年9月)、ー蛋白質研究の最前線ー、と題して(参加者約250名):第71回生化学会(平成10年10月).ー原子レベルの立体構造にもとずく生物マシーナリーの動作機構解明ー、と題して(参加者約70名)、4)欧米における関連分野の情勢の視察(平成10年11月;班員田中勲、田之倉優による;6ケ所の研究室を訪問;その結果は次項のワークショップで報告された)、5)第1回ワークショップの開催:平成11年1月、東大・山上会館、ー「生物マシーナリー」特定領域をどう進めるかー、と題して(参加者約70名)、6)第1回総括班会議の開催:前記のワークショップ中に開催(出席者10名)、7)ホームページの開設:URL=http://bio.nagaokaut.ac.jp/tokutei/第1頁目を長岡技術科学大学に置く).

Research Products
(24 results)

All Other

All Publications (24 results)

[Publications] Tanabe,T.,Tanaka,N.,Nonaka,T.,Mitsui,Y.,Yoshimoto,T.et al.: "Roles of the Ser146,Tyr159 and Lys163 n the catalytic action of 7α-hydroxysteroid dehydrogenase." J.Biochem.(Tokyo). 124. 634-641 (1998)
[Publications] Sugimoto,K.,Aoshima,H.,Senda,T.,Masai,E.,Fukuda,M.& Mitsui,Y: "Purification and crystallization of a protocatecuate 4,5-dioxygenase LigAB from Sphingomonas paucimobilis SYK-6." Protein and Peptide Letters. (in Press). (1999)
[Publications] Sugimoto,K.,Senda,T.,Aoshima,H.Masai,E.,Fukuda,M.& Mitsui,Y.: "Crystal structure of an aromatic-ring-opening dioxygenase LigAB which is a protocatechuate 4,5-dioxygenase." Structure. (in Press). (1999)
[Publications] H.Sakai & T.Tsukihara: "Structures of Membrane Proteins Determined at Atomic Resolution" J.Biochemistry. 124. 1051-1059 (1998)
[Publications] T.Tsukihara: "Crystal Structural Studies of a Membrane Protein Complex,Cytochrome c Oxidase from Bovine Heart." Acta Crystallogr.A54. 895 (1998)
[Publications] S.Yoshikawa et al.: "Redox-Coupled Crystal Structural Change in Bovine Herart Cytochrome c Oxidase." Science. 280. 1723-1729 (1998)
[Publications] T.T.Tahirov et al.: "Crystal Structure of Methionine Aminopeptidase from Hyperthermophile,Pyrococcus furiosus." J.Mol.BIol.284. 101-124 (1998)
[Publications] T.H.Tahirov et al.: "High-Resolution Crystal of Methionine Aminopeptidase from Pyrococcus furiosus Obtained by Water-Mediated Transformation" J.,Structural Biology. 121. 68-72 (1998)
[Publications] I.Tanaka et al.,: "Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA." RNA. 4. 542-555 (1998)
[Publications] J.Nishihira et al.,: "Molecular cloning of human D-dopachrome tautomerase cDNA:N-terminal proline is essential for enzyme activation" Biochem.Biophys.Res.Comm.243. 538-544 (1998)
[Publications] K.Kitadokoro et al.: "Crystal Structure of Human Secretory Phospholipase A_2-IIA Complex with the Potent Indolizine Inhibitor 120-1032" J.Biochem.123. 619-623 (1998)
[Publications] K.Kitano et al.: "A Novel Approach to Crystallizing Proteins with Temperature Induction Method:GrpE Protein from Thermus thermophilus" J.Cryst.Growth. 186. 456-460 (1998)
[Publications] A.Osyczka et al.: "Interaction Site for Soluble Cytochromes on the Tetraheme Cytochrome Subunit Bound to the Bacterial Photosynthetic Reaction Center Mapped by Site-Directed Mutagenesis" Biochemistry. 37. 11732-11744 (1998)
[Publications] H.Komori et al.: "Crystallization and Preliminary X-ray Diffraction Studies of a Replication Intiator Protein (RepE54) of the Mini-F Plasmid Complexes with Iteron DNA" J.Biochem.125. 24-26 (1999)
[Publications] A.Kita et al.: "An archetypical extradiol-cleaving catecholic dioxygenase:the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase)" Structure. 7. 25-34 (1999)
[Publications] Zenno,S.: "Conversion of NfsA,the major Escherichia coli nitroreductase,to a flavin reductase having a specific activity similar to that of Vibrio harbeyi Frp,by a signale amino acid substitution." J.Bacteriol.180. 422-425 (1998)
[Publications] Kono,H.: "Designing the hydrophobic core of Thermus flavus malate dehydrogenase based on side-chain packing." Protein Engineering. 11. 47-52 (1998)
[Publications] Kudo,N.: "Crystallization and preliminary x-ray diffraction studies of a rice cysteine proteinase inhibitor,oryzacystatin-I." J.Biochem.123. 568-570 (1998)
[Publications] Koike,H.: "1.8 Å crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium,Vibrio fischeri:overall structure,cofactor and substrate-analog binding,and comparison with related flavoproteins." J.Mol.Biol.280. 259-273 (1998)
[Publications] Suzuki,Y.: "Evidence for existence of multiple conformations of kinesin and ncd motor domains in solution revealed by 31P NMR of the tightly bound ADP." Eur.J.Biochem.257. 466-471 (1998)
[Publications] P.Zhang: "Structure of the calcium pump from sarcoplasmic reticulum at 8Å resolution" Nature. 392. 835-839 (1998)
[Publications] H.Ogawa: "Structure of the Ca^<2+> pump of sarcoplasmic reticulum:A view along the lipid bilayer at 9-Å resolution" Biophys.J.75. 41-52 (1998)
[Publications] K.Mayanagi: "Three-dimensional electron microscopy of the photosystem II core complex." J.Struct.Biol.(in press). (1998)
[Publications] 三井幸雄、千田俊哉、野中孝昌: "「遺伝」 52巻、7号" X線で見る-生体高分子から生物マシーナリーへ-, 6 (1998)

1998 Fiscal Year Annual Research Report

シンクロトロン放射光による生物マシーナリーの構造生物学

Principal Investigator

三井 幸雄 長岡技術科学大学, 工学部, 教授 (40012637)

Research Products

[Publications] Tanabe,T.,Tanaka,N.,Nonaka,T.,Mitsui,Y.,Yoshimoto,T.et al.: "Roles of the Ser146,Tyr159 and Lys163 n the catalytic action of 7α-hydroxysteroid dehydrogenase." J.Biochem.(Tokyo). 124. 634-641 (1998)

[Publications] Sugimoto,K.,Aoshima,H.,Senda,T.,Masai,E.,Fukuda,M.& Mitsui,Y: "Purification and crystallization of a protocatecuate 4,5-dioxygenase LigAB from Sphingomonas paucimobilis SYK-6." Protein and Peptide Letters. (in Press). (1999)

[Publications] Sugimoto,K.,Senda,T.,Aoshima,H.Masai,E.,Fukuda,M.& Mitsui,Y.: "Crystal structure of an aromatic-ring-opening dioxygenase LigAB which is a protocatechuate 4,5-dioxygenase." Structure. (in Press). (1999)

[Publications] H.Sakai & T.Tsukihara: "Structures of Membrane Proteins Determined at Atomic Resolution" J.Biochemistry. 124. 1051-1059 (1998)

[Publications] T.Tsukihara: "Crystal Structural Studies of a Membrane Protein Complex,Cytochrome c Oxidase from Bovine Heart." Acta Crystallogr.A54. 895 (1998)

[Publications] S.Yoshikawa et al.: "Redox-Coupled Crystal Structural Change in Bovine Herart Cytochrome c Oxidase." Science. 280. 1723-1729 (1998)

[Publications] T.T.Tahirov et al.: "Crystal Structure of Methionine Aminopeptidase from Hyperthermophile,Pyrococcus furiosus." J.Mol.BIol.284. 101-124 (1998)

[Publications] T.H.Tahirov et al.: "High-Resolution Crystal of Methionine Aminopeptidase from Pyrococcus furiosus Obtained by Water-Mediated Transformation" J.,Structural Biology. 121. 68-72 (1998)

[Publications] I.Tanaka et al.,: "Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA." RNA. 4. 542-555 (1998)

[Publications] J.Nishihira et al.,: "Molecular cloning of human D-dopachrome tautomerase cDNA:N-terminal proline is essential for enzyme activation" Biochem.Biophys.Res.Comm.243. 538-544 (1998)

[Publications] K.Kitadokoro et al.: "Crystal Structure of Human Secretory Phospholipase A_2-IIA Complex with the Potent Indolizine Inhibitor 120-1032" J.Biochem.123. 619-623 (1998)

[Publications] K.Kitano et al.: "A Novel Approach to Crystallizing Proteins with Temperature Induction Method:GrpE Protein from Thermus thermophilus" J.Cryst.Growth. 186. 456-460 (1998)

[Publications] A.Osyczka et al.: "Interaction Site for Soluble Cytochromes on the Tetraheme Cytochrome Subunit Bound to the Bacterial Photosynthetic Reaction Center Mapped by Site-Directed Mutagenesis" Biochemistry. 37. 11732-11744 (1998)

[Publications] H.Komori et al.: "Crystallization and Preliminary X-ray Diffraction Studies of a Replication Intiator Protein (RepE54) of the Mini-F Plasmid Complexes with Iteron DNA" J.Biochem.125. 24-26 (1999)

[Publications] A.Kita et al.: "An archetypical extradiol-cleaving catecholic dioxygenase:the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase)" Structure. 7. 25-34 (1999)

[Publications] Zenno,S.: "Conversion of NfsA,the major Escherichia coli nitroreductase,to a flavin reductase having a specific activity similar to that of Vibrio harbeyi Frp,by a signale amino acid substitution." J.Bacteriol.180. 422-425 (1998)

[Publications] Kono,H.: "Designing the hydrophobic core of Thermus flavus malate dehydrogenase based on side-chain packing." Protein Engineering. 11. 47-52 (1998)

[Publications] Kudo,N.: "Crystallization and preliminary x-ray diffraction studies of a rice cysteine proteinase inhibitor,oryzacystatin-I." J.Biochem.123. 568-570 (1998)

[Publications] Koike,H.: "1.8 Å crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium,Vibrio fischeri:overall structure,cofactor and substrate-analog binding,and comparison with related flavoproteins." J.Mol.Biol.280. 259-273 (1998)

[Publications] Suzuki,Y.: "Evidence for existence of multiple conformations of kinesin and ncd motor domains in solution revealed by 31P NMR of the tightly bound ADP." Eur.J.Biochem.257. 466-471 (1998)

[Publications] P.Zhang: "Structure of the calcium pump from sarcoplasmic reticulum at 8Å resolution" Nature. 392. 835-839 (1998)

[Publications] H.Ogawa: "Structure of the Ca^<2+> pump of sarcoplasmic reticulum:A view along the lipid bilayer at 9-Å resolution" Biophys.J.75. 41-52 (1998)

[Publications] K.Mayanagi: "Three-dimensional electron microscopy of the photosystem II core complex." J.Struct.Biol.(in press). (1998)

[Publications] 三井幸雄、千田俊哉、野中孝昌: "「遺伝」 52巻、7号" X線で見る-生体高分子から生物マシーナリーへ-, 6 (1998)

三井幸雄長岡技術科学大学, 工学部, 教授 (40012637)