遺伝的組換えの新展開:DNAの切断と再結合

2000 Fiscal Year Annual Research Report

• Project/Area Number: 10216101
• Research Institution: Kitasato Institute
• Principal Investigator: 池田 日出男 社団法人北里研究所, 基礎研究所, 部長 (10012775)
• Co-Investigator(Kenkyū-buntansha): 小川 英行 岩手女子看護短期大学, 学長 (70028207) ; 関口 睦夫 福岡歯科大学, 歯学部, 教授 (00037342) ; 志村 令郎 生物分子工学研究所, 所長 (60025426) ; 飯田 滋 基礎生物学研究所, 教授 (30012777) ; 大坪 栄一 東京大学, 分子細胞生物学研究所, 教授 (10158800)
• Keywords: DNAエンドジョイニング / 遺伝的組換え / DNAの切断と再結合 / トランスポゾン / ブルーム症候群 / RecQヘリカーゼ / シャフロン / 遺伝子ホーミング

Research Abstract

本特定領域研究は、非相同的組換えに焦点を絞り、DNAエンドジョイニングやトランスポゾンを含むいわゆる動く遺伝子の転移機構を「DNAの切断と再結合」という観点から総合的に理解しようとするものである。本年度は、8月に班会議、12月にワークショップ「遺伝的組換えとその制御」、3月に国際シンポジウム「ジェネテイクスとエピジェネテイクスの進歩」を開催し、各計画研究間の連携や外国との交流を諮った。国際シンポジウムには、21名の外国人研究者と13名の日本人研究者を迎えて、話題提供と活発な討論が行われ、活気のある国際集会となった。昨年に比べて各班員の研究は著しく進展しており、この領域の研究の発展に寄与している。そのなかで特筆すべきものは飯田班員による研究で、Tpn1ファミリーのトランスポゾンの挿入によるアサガオの未知遺伝子の同定法を確立し、開花時に花弁細胞の液胞内のpHを調節して、花色を紫色から青色に変化させる新規遺伝子の同定に成功した。これは、液胞pHを上昇させて花色を青色化させる遺伝子の機能が明らかにされた最初の例である。また、近藤班員は、ヒトBLM(ブルーム症候群原因タンパク質)とATMとの間に相互作用があり、ATMがBLMのN-末端部位をリン酸化しBLMの機能を調節していることを見いだした。班員、或いは、班友の間での連携も極めて活発である。池田班員が解析した大腸菌RecQタンパク質の機能の成果は、大坪班員のトランスポゾンの研究、立花班員のヒトBLMタンパク質の研究、飯田班員の植物細胞の相同組換えにおける大腸菌RecQタンパク質の研究などに寄与している。班員の研究推進のために大型機器が購入され、今年度は国立基礎生物学研究所に設置された。研究業績の公開のためにホームページを更新し、公開している。今後も、さらに公開シンポジウムを企画し、研究業績の公開を行いたいと考えている。

Research Products

• [Publications] Yamaguchi,H.,Hanada,K.,Asami,Y.,Kato,J.and Ikeda,H.: "Control of genetic stability in Escherichia coli : the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease."Genes Cells. 5. 101-109 (2000)
• [Publications] Hanada,K.,Iwasaki,M.,Ihashi,S.and Ikeda,H.: "UvrA and UvrB suppresses illegitimate recombination : Synergistic action with RecQ helicase."Proc.Natl.Acad.Sci.USA. 97. 5989-5994 (2000)
• [Publications] Shanado,Y.,Hanada,K.and Ikeda,H.: "Suppression of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protem H-NS."Mol.Gen.Genet.. (印刷中).
• [Publications] Tatebayashi,K.,Tani,T.and Ikeda,H.: "Fission yeast Mog1p homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly (A) ^+ RNA metabolism."Genetics. (印刷中).
• [Publications] Kang,S.-W.and Ikeda,H.: "WRN homologues in non-human systems.In Werner syndrome : Implications for aging and cancer."GANN MONOGRAPH ON CANCER RESEARCH. (印刷中).
• [Publications] Onda,M.and Ikeda,H.: "Role of DNA ligase in the illegitimate recombination that generates bio transducing phages."Genetics. (印刷中).
• [Publications] Takeda,T.,Ogino,K.,Tatebayashi,K.,Ikeda,H.,Arai,K.and Masai,H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast."Mol.Biol.Cell.. (印刷中).
• [Publications] Shobuike,T.,Tatebayashi,K.,Tani,T.,Sugano,S.and Ikeda,H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast."Nucleic Acid Res.. (印刷中).
• [Publications] Kanaya,E.,Watanabe,K.,Nakajima,N.,Okada,K.and Shimura,Y.: "Zinc release from the CH2C6 zinc finger domain of FILAMENTOUS FLOWER protein from arabidopsis thaliana induces self-assembly."J Biol Chem.. 276(10). 7383-7390 (2001)
• [Publications] Shiraishi,A.,Sakumi,K.and Sekiguchi,M.: "Increased susceptibility to chemotherapeutic alkylating agents of mice deficient in DNA repair methyltransferase."Carcinogenesis. 21(10). 1879-1883 (2000)
• [Publications] Shimokawa,H.,Fujii,Y.,Furuichi,M.,Sekiguchi,M.and Nakabeppu,Y.: "Functional significance of conserved residues in the phosphohydrolase module of escherichia coli MutT protein."Nucleic Acids Res.. 28. 3240-3249 (2000)
• [Publications] Miyako,K.,Takamatsu,C.,Umeda,S.,Tajiri,T.,Furuichi,M.,Nakabeppu,Y.,Sekiguchi,M., et al.: "Accumulation of adenine DNA glycosylase-sensitive sites in human mitochondrial DNA."J Biol Chem.. 275. 12326-12330 (2000)
• [Publications] Inoue,R.,Abe,M.,Nakabeppu,Y.,Sekiguchi,M.,Mori,T.and Suzuki,T.: "Characterization of human polymorphic DNA repair methyltransferase."Pharmacogenetics. 10. 59-66 (2000)
• [Publications] Kawate,H.,Itoh,R.,Sakumi,K.,Nakabeppu,Y.,Tsuzuki,T.,Ide,F.,Ishikawa,T.,Noda,T.,Nawata,H.and Sekiguchi,M.: "A defect in a single allele of the Mlh1 gene causes dissociation of the killing and tumorigenic actions of an alkylating carcinogen in methyltransferase-deficient mice."Carcinogenesis. 21. 301-305 (2000)
• [Publications] Tsubouchi,H.and Ogawa,H.: "Exo1 roles for repair of DNA double-strand breaks and meiotic crossing over in Saccharomyces cerevisiae."Mol Biol Cell.. 11. 2221-2233 (2000)
• [Publications] Shcherbakova,OG.,Lanzov,VA.,Ogawa,H.and Filatov,MV.: "Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells."Mutat Res.. 459. 65-71 (2000)
• [Publications] Cheng,C.,Tsuchimoto,S.,Ohtsubo,H.and Ohtsubo,E.: "Tnr8, a foldback transposable element from rice."Genes Genet.Syst.. 75. 327-333 (2000)
• [Publications] Noma,K.and Ohtsubo,E.: "Tnat1 and Tnat2 from Arabidopsis thaliana : novel transposable elements with tandem repeat sequences."DNA Res.. 7. 1-7 (2000)
• [Publications] Han,C.-G.,Frank,M.J.,Ohtsubo,H.and Ohtsubo,E.: "New transposable elements identified as insertions in rice transposon Tnr1."Genes Genet.Syst.. 75. 69-77 (2000)
• [Publications] Noma,K.,Ohtsubo,H.and Ohtsubo,E.: "ATLN elements, LINEs from Arabidopsis thaliana : Identification and characterization."DNA Res.. 7. 291-303 (2000)
• [Publications] Tsuchimoto,S.,Mayama,T.,van der Krol,A.and Ohtsubo,E.: "The whorl-specific action of a petunia class B floral homeotic gene."Genes Cells. 5. 89-99 (2000)
• [Publications] Hayashi,T.,Makino,K.,Ohnishi,M.,Kurokawa,K.,Ishii,K.,Yokoyama,K.,Han,C.-G.,Ohtsubo,E., et al.: "Genome sequence and comparative analysis of enterohemorrhagic E.coli O157:H7."DNA Res.. (印刷中).
• [Publications] Shiga,Y.,Sekine,Y.,Kano,Y.and Ohtsubo,E.: "The involvement of H-NS in transpositional recombination mediated by IS1."J.Bacteriol.. (印刷中).
• [Publications] Kumekawa,N.,Ohmido,N.,Fukui,K.,Ohtsubo,E.and Ohtsubo H.: "A new gypsy-type retrotransposon RIRE7:preferential insertion into the tandem repeat sequence TrsD in pericentromic heterochromatin regions of rice chromosomes."Mol.Gen.Genet.. (印刷中).
• [Publications] Myouga,F.,Tsuchimoto,S.,Ohtsubo H.and Ohtsubo,E.: "Transcriptional analysis of the Mu-like element Tnr2 from rice."International Rice Research Notes. (印刷中).
• [Publications] Fukada-Tanaka,S.,Inagaki,Y.,Yamaguchi,T.Saito N.and Iida,S.: "Colour-enhancing protein in blue petals."Nature. 407. 581 (2000)
• [Publications] Hasebe A.and Iida,S.: "The novel insertion sequences IS1417, IS1418 and IS1419 from Burkholderia glumae and their strain distribution."Plasmid. 44. 44-53 (2000)
• [Publications] Iida S.,Hoshino,A.and Choi,J.-D.: "Floricultural traits and transposable elements in morning glories."Kor.J.Hort.Sci.Tech.. 18. 845-848 (2000)
• [Publications] Kojima,T.,Habu,Y.,Iida S.and Ogihara,Y.: "Direct isolation of differentially expressed genes from a specific chromosome region of common wheat : Application of the amplified fragment length polymorphism-based mRNA fingerprinting (AMF) method in combination with a deletion line of wheat."Mol.Gen.Genet.. 263. 635-641 (2000)
• [Publications] Shiokawa,K.,Inagaki,Y.,Morita,H.,Hsu,T.-J.,Iida S.and Noguchi,H.: "The functional expression of the CHS-D and CHS-E genes of the common morning glory (Ipomoea purpurea) in Escherichia coli and characterization of their gene products."Plant Biotech.. 17. 203-210 (2000)
• [Publications] Hoshino,A.,Johzuka-Hisatomi Y.and Iida,S.: "Gene duplication and mobile genetic elements in the morning glories."Gene. (印刷中).
• [Publications] Fukada-Tanaka,S.,Inagaki,Y.,Yanaguchi T.and Iida,S.: "Simplified transposon display (STD) : a new procedure for isolation of a gene tagged by a transposable element belonging to the Tpn1 family in the Japanese morning glory."Plant Biotech.. (印刷中).
• [Publications] Iida S.and Hoshino,A.: "Spontaneous mutagenesis and transposable elements in the Japanese morning glory."Gamma Field Symposia. (印刷中).