2002 Fiscal Year Annual Research Report

道管形成過程での液胞崩壊の研究

Research Project

Project/Area Number	10219201
Research Institution	The University of Tokyo
Principal Investigator	馳澤盛一郎東京大学, 大学院・新領域創成科学研究科, 教授 (40172902)
Co-Investigator(Kenkyū-buntansha)	福田裕穂東京大学, 大学院・理学系研究科, 教授 (10165293)
Keywords	液胞 / プログラム細胞死 / 管状要素 / ヒャクニチソウ / タバコBY-2 / ヌクレアーゼ / TVM / AtVam3p
Research Abstract	我々は高等植物細胞の形態形成における液胞の寄与について研究を進めている。今年度は、液胞の立体構造の詳細な経時観察のため、シロイヌナズナのsyntaxinファミリーに属するAt Vam3pとGFPの融合タンパク質を恒常的に発現するタバコBY-2細胞の形質転換株BY-GV(transgenic <BY>-2 cells stably expressing GFP-AtVam3p fusion protein)を確立した。このBY-GV細胞を用いて、共焦点レーザー顕微鏡によって得られた連続光学切片をもとに、新規に独自で開発した立体画像構築ソフトSSR(stereo-structure reconstructor)による立体画像構築とモデリングを行った。その結果、TVM(tubular structure of vacuolar membrane)を含む液胞構造の立体的な構成と、細胞周期の進行に伴う動態の全容が明らかになった。また我々は、これまでにヒャクニチソウ葉肉細胞から管状要素への分化転換系において、道管形成の分子機構について詳細な解析を行い、液胞が細胞の自己分解過程で崩壊することによりプログラム細胞死の中心的役割を担うことを明らかにしてきた。特に、細胞死特異的発現を示すS1ヌクレアーゼ遺伝子(ZEN1)の産物が液胞に特異的に蓄積されること、液胞崩壊後の核分解に直接関与していることなどを示してきた。本年度は、これ以外にCa/Mg依存ヌクレアーゼが細胞死の時に一過的に発現することを明らかにした。また、ZEN1以外の細胞死特異的システインプロテアーゼZCP4,細胞死特異的ヌクレアーゼZeRNase1が、分化中の管状要素でのみ、小胞体、ゴルジ体を経由して液胞に蓄積することを明らかにした。

Research Products

(7 results)

All Other

All Publications (7 results)

[Publications] 馳澤盛一郎: "Dynamic changes and roles of cytoskeleton during cell cycle in higher plant cells"International Review of Cytology. 214. 161-191 (2002)
[Publications] 柳川由紀: "Cell-Cycle Dependent Dynamic Change of 26S Proteaso me Distribution in Tobacco BY-2 Cells"Plant & Cell Physiology. 43. 604-613 (2002)
[Publications] 朽名夏麿: "Dynamic organization of vacuolar and microtubule structures during cell cycle progression in synchronized tobacco BY-2 cells"Plant & Cell Physiology. 43. 965-973 (2002)
[Publications] 中名生幾子: "Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans"Plant & Cell Physiology. 43. 1484-1492 (2002)
[Publications] 井藤純: "ZEN1 is a key enzyme in degradation of nuclear DNA during programmed cell death of tracheary elements"Plant Cell. 14. 3201-3211 (2002)
[Publications] 出村拓: "Visualization by comprehensive microarray analysis of gene expression programs during transdifferentition of mesophyll cells"Proc.Natl.Acad.Sci.USA.. 99. 15794-15799 (2002)
[Publications] 熊谷史: "γ-Tubulin distribution during cortical microtubule reor ganization at the M/G_1 interface in tobacco BY-2 cells"European Journal of Cell Biology. 82. 43-51 (2003)

2002 Fiscal Year Annual Research Report

道管形成過程での液胞崩壊の研究

Principal Investigator

馳澤 盛一郎 東京大学, 大学院・新領域創成科学研究科, 教授 (40172902)

Research Products

[Publications] 馳澤 盛一郎: "Dynamic changes and roles of cytoskeleton during cell cycle in higher plant cells"International Review of Cytology. 214. 161-191 (2002)

[Publications] 柳川 由紀: "Cell-Cycle Dependent Dynamic Change of 26S Proteaso me Distribution in Tobacco BY-2 Cells"Plant & Cell Physiology. 43. 604-613 (2002)

[Publications] 朽名 夏麿: "Dynamic organization of vacuolar and microtubule structures during cell cycle progression in synchronized tobacco BY-2 cells"Plant & Cell Physiology. 43. 965-973 (2002)

[Publications] 中名生 幾子: "Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans"Plant & Cell Physiology. 43. 1484-1492 (2002)

[Publications] 井藤 純: "ZEN1 is a key enzyme in degradation of nuclear DNA during programmed cell death of tracheary elements"Plant Cell. 14. 3201-3211 (2002)

[Publications] 出村 拓: "Visualization by comprehensive microarray analysis of gene expression programs during transdifferentition of mesophyll cells"Proc.Natl.Acad.Sci.USA.. 99. 15794-15799 (2002)

[Publications] 熊谷 史: "γ-Tubulin distribution during cortical microtubule reor ganization at the M/G_1 interface in tobacco BY-2 cells"European Journal of Cell Biology. 82. 43-51 (2003)

馳澤盛一郎東京大学, 大学院・新領域創成科学研究科, 教授 (40172902)

[Publications] 馳澤盛一郎: "Dynamic changes and roles of cytoskeleton during cell cycle in higher plant cells"International Review of Cytology. 214. 161-191 (2002)

[Publications] 柳川由紀: "Cell-Cycle Dependent Dynamic Change of 26S Proteaso me Distribution in Tobacco BY-2 Cells"Plant & Cell Physiology. 43. 604-613 (2002)

[Publications] 朽名夏麿: "Dynamic organization of vacuolar and microtubule structures during cell cycle progression in synchronized tobacco BY-2 cells"Plant & Cell Physiology. 43. 965-973 (2002)

[Publications] 中名生幾子: "Preferential and asymmetrical accumulation of a Rac small GTPase mRNA in differentiating xylem cells of Zinnia elegans"Plant & Cell Physiology. 43. 1484-1492 (2002)

[Publications] 井藤純: "ZEN1 is a key enzyme in degradation of nuclear DNA during programmed cell death of tracheary elements"Plant Cell. 14. 3201-3211 (2002)

[Publications] 出村拓: "Visualization by comprehensive microarray analysis of gene expression programs during transdifferentition of mesophyll cells"Proc.Natl.Acad.Sci.USA.. 99. 15794-15799 (2002)

[Publications] 熊谷史: "γ-Tubulin distribution during cortical microtubule reor ganization at the M/G_1 interface in tobacco BY-2 cells"European Journal of Cell Biology. 82. 43-51 (2003)