2000 Fiscal Year Annual Research Report

種分化にかかわる遺伝子の同定と解析

Research Project

Project/Area Number	10304062
Research Institution	Tokyo Metropolitan University
Principal Investigator	布山喜章東京都立大学, 理学研究科, 教授 (20087133)
Co-Investigator(Kenkyū-buntansha)	小熊譲筑波大学, 生物科学系, 教授 (90114074) 磯野邦夫東北大学, 情報科学研究科, 助手 (70124550) 猪股伸幸九州大学, 理学部, 助手 (20301335) 大石陸生神戸大学, 理学部, 教授 (80030782) 木村正人北海道大学, 地球環境科学研究科, 教授 (30091440)
Keywords	種分化遺伝子 / 適応進化 / ホスト適応 / 交尾行動 / 求愛歌と種認識 / 単為生殖 / 人工授精 / 雑種雄不妊
Research Abstract	1.単食性ショウジョウバエD.sechelliaのホスト植物中の有毒成分に対する胚の耐性に卵核膜が重要であること、また、産卵の遅延による卵胎生化も寄与することが判明した。 2.キイロショウジョウバエおよび近縁種のアミラーゼ遺伝子の変異をDNAレベルで調べた結果、自然選択が働いた証拠が得られた。 3.野外集団由来のオナジショウジョウバエ雌のキイロショウジョウバエおよびD.teissier雄との交配率を調べた結果、後者で交雑率の高い系統が得られた。 4.雄の交尾活動の光依存性に変化をもたらす突然変異に、網膜で発現する細胞接着分子chaoptinが関与することがわかった。 5.同所的に分布するD.ananassaeとD.pallidaosaの求愛歌の分析の結果、パルス間間隔(IPI)に差は見られず、2種間の種認識にIPIは関わっていないと結論された。 6.カザリショウジョウバエの黒色、褐色系統間に見られる交尾選択に、温度適応および近縁種との出会いが関係する可能性を検討したが、支持する結果は得られなかった。 7.アナナスショウジョウバエ類3種で発見された単為生殖は、すべて第2染色体左腕の同位置にある遺伝子によるもので、2倍体化はpronuclear duplicationによることが細胞学及び遺伝学的に示唆された。 8.膜翅目昆虫の未受精卵への異種精子注入により雑種胚が形成されることを確認したが、成虫に達したものはなかった。一方、同属異種間の卵巣移植により異種卵黄タンパクのみで卵成熟が起こり、人為的に単為発生させると成虫にまで育つことを確認した。 9.雌の貯精嚢で貯精される精子の形成に必要な遺伝子の同定を試み、貯精されるが不妊となる突然変異を8系統確立した。そのうち、卵への進入が不能なものと精子核が膨潤しないものについて細胞学的観察と遺伝子座の決定を行なった。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] Hurst,G.D.D.: "Male-killing Wolbachia in Drosophila : a temperature sensitive trait with a threshold bacterial density."Genetics . 156. 699-709 (2000)
[Publications] Liang,B.-L.: "Can D.simulans breed on Morinda citrifolia, the host plant of D.sechellia?"Drosophila Information Service. 83. (2000)
[Publications] Seong,K.-H.: "Application of the gene search system to a screen for longevity genes in Drosophila."Biogerontology. 2. (2001)
[Publications] Araki,H.: "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster : Evidence of positive selection in the coding regions and selective constraint in the cis-regulatory regions."Genetics. 601-615 (2000)
[Publications] Inomata,N.: "Evolution of nucleotide substitutions and gene regulation in the amylase multigenes in Drosophila kikkawai and its sibling species."Molecular Biology & Evolution. 17. (2001)
[Publications] Tsuchihara,K.: "A putative binding protein for lipophilic substances related to butterfly oviposition."FEBS Letters. 4-78. 299-303 (2000)
[Publications] 磯野邦夫: "ハエの糖リセプター"味と匂学会誌. 7・2. 187-190 (2000)
[Publications] Isono,K.: "Mollecular biological study of the taste gene Tre, a candidate Drosophila sweet taste receptor."Japanese Journal of Smell & Taste Research. 7・3. 301-302 (2000)
[Publications] Ohta,M.: "Sequence analysis of the polymorphism of the candidate sweet taste receptor gene GPCR(5A) in Drosophila."Japanese Journal of Smell & Taste Research. 7・3. 689-690 (2000)
[Publications] Miyo,T.: "Inheritance of resistance to estenvalerate in Colorado potato beetles (Coleoptera : Chrysomelidae)."Journal of Economic Entomology. 92. 1031-1038 (1999)
[Publications] Miyo,T.: "Seasonal fluctuation in susceptibility to insecticides with in natural populations of Drosophila melanogaster : empirical observations of fitness costs of insecticide resistance. "Genes & Genetic Systems. 75. 95-104 (2000)
[Publications] Tomaru,M.: "Courtship song recognition in the Drosophila melanogaster complex : Heterospecific songs make females receptive in Drosophila melanogaster but not in D.sechellia."Evolution. 54. 1286-1294 (2000)
[Publications] Tomaru,M.: "Mate choice in Drosophila melanogaster and D.sechellia : criteria and their variation depending on courtship song."Animal Behaviour. 60. 797-804 (2000)
[Publications] Miyo,T.: "Genetic variation and correlations among responses to five insecticides within natural populations of Drosophila melanogaster (Diptera : Drosophilidae)."Journal of Economic Entomology. 94. 223-232 (2001)
[Publications] Doi,M.: "A locus for female discrimination behavior causing sexual isolation in Drosophila."Proc.Nat.Acad.Sci.USA. (2001)
[Publications] Ishii,K.: "Sexual isolation and cuticular hydrocarbons in Drosophila elegans."Heredity. (2001)
[Publications] Sato,H.: "A site specific increase in recombination in Drosophila ananassae."Genes & Genetic Systems. 75. 41-47 (2000)
[Publications] Hatakeyama,M.: "Artificial reproduction in a hymenopteran insect, Athalia rosae, using eggs matured with heterospecific yolk proteins and fertilized with cryopreserved sperm."Archives of Insect Biochemistry & Physiology. 43. 137-144 (2000)
[Publications] Hatakeyama,M.: "Participation of testicular spermatids in development upon intracytoplasmic injection into eggs of the sawfly, Athalia rosae (Insects, Hymenoptera)."Journal of Experimental Zoology. 286. 181-192 (2000)
[Publications] Lee,J.: "Vitellogenin of the cicada Graptopsaltria nigrofuscata (Homoptera) : analysis of its primary structure."Insect Biochemistry & Molecular Biology. 30. 1-7 (2000)
[Publications] Lee,J.: "A simple and rapid method for cloning insect vitellogenin cDNAs."Insect Biochemistry & Molecular Biology. 30. 189-194 (2000)
[Publications] Tufail,M.: "Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analysis."Archives of Insect Biochemistry & Physiology. 45. 37-46 (2000)
[Publications] 柏原孝夫他(編): "動物遺伝学"文永堂出版. 237 (2000)

2000 Fiscal Year Annual Research Report

種分化にかかわる遺伝子の同定と解析

Principal Investigator

布山 喜章 東京都立大学, 理学研究科, 教授 (20087133)

Research Products

[Publications] Hurst,G.D.D.: "Male-killing Wolbachia in Drosophila : a temperature sensitive trait with a threshold bacterial density."Genetics . 156. 699-709 (2000)

[Publications] Liang,B.-L.: "Can D.simulans breed on Morinda citrifolia, the host plant of D.sechellia?"Drosophila Information Service. 83. (2000)

[Publications] Seong,K.-H.: "Application of the gene search system to a screen for longevity genes in Drosophila."Biogerontology. 2. (2001)

[Publications] Araki,H.: "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster : Evidence of positive selection in the coding regions and selective constraint in the cis-regulatory regions."Genetics. 601-615 (2000)

[Publications] Inomata,N.: "Evolution of nucleotide substitutions and gene regulation in the amylase multigenes in Drosophila kikkawai and its sibling species."Molecular Biology & Evolution. 17. (2001)

[Publications] Tsuchihara,K.: "A putative binding protein for lipophilic substances related to butterfly oviposition."FEBS Letters. 4-78. 299-303 (2000)

[Publications] 磯野邦夫: "ハエの糖リセプター"味と匂学会誌. 7・2. 187-190 (2000)

[Publications] Isono,K.: "Mollecular biological study of the taste gene Tre, a candidate Drosophila sweet taste receptor."Japanese Journal of Smell & Taste Research. 7・3. 301-302 (2000)

[Publications] Ohta,M.: "Sequence analysis of the polymorphism of the candidate sweet taste receptor gene GPCR(5A) in Drosophila."Japanese Journal of Smell & Taste Research. 7・3. 689-690 (2000)

[Publications] Miyo,T.: "Inheritance of resistance to estenvalerate in Colorado potato beetles (Coleoptera : Chrysomelidae)."Journal of Economic Entomology. 92. 1031-1038 (1999)

[Publications] Miyo,T.: "Seasonal fluctuation in susceptibility to insecticides with in natural populations of Drosophila melanogaster : empirical observations of fitness costs of insecticide resistance. "Genes & Genetic Systems. 75. 95-104 (2000)

[Publications] Tomaru,M.: "Courtship song recognition in the Drosophila melanogaster complex : Heterospecific songs make females receptive in Drosophila melanogaster but not in D.sechellia."Evolution. 54. 1286-1294 (2000)

[Publications] Tomaru,M.: "Mate choice in Drosophila melanogaster and D.sechellia : criteria and their variation depending on courtship song."Animal Behaviour. 60. 797-804 (2000)

[Publications] Miyo,T.: "Genetic variation and correlations among responses to five insecticides within natural populations of Drosophila melanogaster (Diptera : Drosophilidae)."Journal of Economic Entomology. 94. 223-232 (2001)

[Publications] Doi,M.: "A locus for female discrimination behavior causing sexual isolation in Drosophila."Proc.Nat.Acad.Sci.USA. (2001)

[Publications] Ishii,K.: "Sexual isolation and cuticular hydrocarbons in Drosophila elegans."Heredity. (2001)

[Publications] Sato,H.: "A site specific increase in recombination in Drosophila ananassae."Genes & Genetic Systems. 75. 41-47 (2000)

[Publications] Hatakeyama,M.: "Artificial reproduction in a hymenopteran insect, Athalia rosae, using eggs matured with heterospecific yolk proteins and fertilized with cryopreserved sperm."Archives of Insect Biochemistry & Physiology. 43. 137-144 (2000)

[Publications] Hatakeyama,M.: "Participation of testicular spermatids in development upon intracytoplasmic injection into eggs of the sawfly, Athalia rosae (Insects, Hymenoptera)."Journal of Experimental Zoology. 286. 181-192 (2000)

[Publications] Lee,J.: "Vitellogenin of the cicada Graptopsaltria nigrofuscata (Homoptera) : analysis of its primary structure."Insect Biochemistry & Molecular Biology. 30. 1-7 (2000)

[Publications] Lee,J.: "A simple and rapid method for cloning insect vitellogenin cDNAs."Insect Biochemistry & Molecular Biology. 30. 189-194 (2000)

[Publications] Tufail,M.: "Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analysis."Archives of Insect Biochemistry & Physiology. 45. 37-46 (2000)

[Publications] 柏原孝夫 他(編): "動物遺伝学"文永堂出版. 237 (2000)

布山喜章東京都立大学, 理学研究科, 教授 (20087133)

[Publications] 柏原孝夫他(編): "動物遺伝学"文永堂出版. 237 (2000)