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2000 Fiscal Year Final Research Report Summary

Identification and Analyses of Genes Involved in the Speciation

Research Project

Project/Area Number 10304062
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionTokyo Metropolitan University

Principal Investigator

FUYAMA Yoshiaki  Tokyo Metropolitan University, Graduate School of Science, Professor, 理学研究科, 教授 (20087133)

Co-Investigator(Kenkyū-buntansha) OGUMA Yuzuru  University of Tsukuba, Institute of Biological Sciences, Professor, 生物科学系, 教授 (90114074)
ISONO Kunio  Tohoku University, Graduate School, of Information Science, Instructor, 情報科学研究科, 助手 (70124550)
INOMATA Nobuyuki  Kyushu University, Faculty of Science, Instructor, 理学部, 助手 (20301335)
OISHI Kugao  University, Faculty of Science, Professor, 理学部, 教授 (80030782)
KIMURA Masahito  Hokkaido University, Graduate School of Environmental Earth Science, Professor, 地球環境科学研究科, 教授 (30091440)
Project Period (FY) 1998 – 2000
Keywordsspeciation genes / host choice / fertilization gene / courtship song / mating behavior / parthenogenesis / amylase gene / artificial insemination
Research Abstract

1) Egg chorion was found to play an important role in the protection of embryos from toxic substances contained in the host plant of a monophagous Drosophila, D.sechellia.
2) Comparisons of amylase genes among D.melanogaster and related species provided evidence for natural selection.
3) Interspecific mating between Drosophila simulans females and males of closely related species was shown to be controlled by a number of species-specific, semidominant genes.
4) Light dependent mating activity of Drosophila male was shown to be determined by male visual acuity and was wavelength specific. The optical property of photo-receptor cells in the compound eye is involved in the visual information about mating.
5) Courtship songs of two sympatric Drosophila, D.ananassae and D.pallidosa were analyzed. It was concluded that Interpulse Interval (IPI) is not important in species recognition between the two species.
6) Cuticular unsaturated fatty acids and other unidentified sex pheromones was seemed to control mate choice found between the black and brown strains of Drosophila elegans.
7) Parthenogenesis discovered in 3 species of ananassae complex of Drosophila was shown to be caused by a gene located in common on the right arm of the second chromosome. Pronuclear duplication was suggested for the mechanism of diploidization.
8) In several hymenopteran insects, injection of heterospecific sperm into an unfertilized egg resulted in hybrid embryos which, however, failed to develop until adults. Heterospecific transplantation of ovary showed that yolk protein of different species could support normal development.
9) Mutants of D.melanogaster that were defective in spermatogenesis and fertilization were isolated, and several genes necessary for sperm storage in the female and for pronuclear fusion after fertilization were identified.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Seki,T.: "The metabolic pathway of visual pigment chromophore formation in Drosophila melanogaster : all-trans (3S)-3-hydroxyretinal is formed from all-trans retinal via (3R)-3-hydroxyretinal in the dark."European Journal of Biochemistry. 257. 522-527 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomaru,M.: "Effects of courtship song in inter-specific crosses among the species of the Drosophila auraria complex."Journal of Insect Behavior. 11. 383-398 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda,M.: "A parthenogenetic strain of Drosophila pallidosa-like in the Drosophila ananassae complex.."Drosophila Information Service. 82. 49-50 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo,Y.: "Evolution of the amylase isozymes in Drosophila melanogaster species subgroup."Biochemical Genetics. 37. 289-300 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okano,S.: "A putative blue-light receptor from Drosophila melanogaster."Photochemistry & Photobiology. 69. 108-113 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama,M.: "Artificial reproduction in a hymenopteran insect, Athalia rosae, using eggs matured with heterospecific yolk proteins nad fertilized with cryopreserved sperm."Archives of Insect Biochemistry & Physiology. 43. 137-144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama,M.: "Participation of testicular spermatids in development upon intracytoplasmic injection into eggs of the sawfly, Athalia rosae (Insects, Hymenoptera)."Journal Experimental Zoology. 286. 181-192 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inomata,N.: "Evolution of nucleotide substitutions and gene regulation in the amylase multigenes in Drosophila kikkawai and its sibling species."Molecular Biology & Evolution. 17. 601-615 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee,J.M.: "Vitellogenin of the cicada Graptopsaltria nigrofuscata (Homoptera) : analysis of its primary structure."Insect Biochemistry & Molecular Biology. 30. 1-7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee,J.M.: "A simple and rapid method for cloning insect vitellogenin cDNAs."Insect Biochemistry & Molecular Biology. 30. 189-194 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Liang,B.-L.: "Can D.simulans breed on Morinda citrifolia, the host plant of D.sechellia?"Drosophila Information Service. 83. (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomaru,M.: "Courtship song recognition in the Drosophila melanogaster complex : Heterospecific songs make females receptive in Drosophila melanogaster but not in D.sechellia."Evolution. 54. 1286-1294 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomaru,M.: "Mate choice in Drosophila melanogaster and D.sechellia : criteria and their variation depending on courtship song."Animal Behaviour. 60. 797-804 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tufail,M.: "Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analysis."Archives of Insect Biochemistry & Physiology. 45. 37-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Araki,H.: "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster : Evidence of positive selection in the coding regions and selective constraint in the cis-regulatory regions."Genetics. 157. 667-677 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doi,M.: "A locus for female discrimination behavior causing sexual isolation in Drosophila."Procedings National Academy of Science USA. (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,K.: "Sexual isolation and cuticular hydrocarbons in Drosophila elegans."Heredity. (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seki, T., K.Isono, . K.Ozaki, Y.Tsukahara, Y.Shibata-Katsuta, M.Ito, T.Irie & M.Katagiri: "The metabolic pathway of visual pigment chromophore formation in Drosophila melanogaster : all-trans (3S)-3-hydroxyretinal is formed from all-trans retinal via (3R)-3-hydroxyretinal in the dark."Eur.J.Biochem.. 257. 522-527 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomaru, M., H.Matsubayashi & Y.Oguma: "Effects of courtship song in inter-specific crosses among the species of the Drosophila auraria complex."J.Insect Behavior. 11. 383-398 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, M.& Y.N.Tobari: "A parthenogenetic strain of Drosophila pallidosa-like in the Drosophila ancnassae complex."Dros.Inf.Serv. 82. 49-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo, Y., N.Inomata & T.Yamazaki: "Evolution of the amylase isozymes in Drosophila melanogaster species subgroup.Biochem."Genet. 37. 289-300 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okano, S., S.Kanno, M.Takao, A.P.M.Eker, K.Isono, Y.Tsukahara & A.Yasui: "A putative blue-light receptor from Drosophila melanogaster"Photochem. Photobiol. 69. 108-113 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama, M., J.M.Lee, M.Sawa, & K.Oishi: "Artificial reproduction in a hymenopteran insect, Athalia rosae, using eggs matured with heterospecific yolk proteins and fertilized with cryopreserved spem"Arch.Insect Biochem.Physiol. 43. 137-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama, M., Y.Nishimori & K.Oishi: "Participation of testicular spermatids in development upon intracytoplasmic injection into eggs of the sawfly, Athalia rosae (Insects, Hymenoptera)"J.Exp.Zool.. 286. 181-192 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inomata, N.& T.Yamazaki: "Evolution of nucleotide substitutions and gene regulation in the amylase multigenes in Drosophila kikkawai and its sibling species"Mol.Biol.Evol.. 17. 601-615 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee, J.M., Y., Nishimori, M.Hatakeyama, T-W.Bae & K.Oishi: "Vitellogenin of the cicada Graptopsaltria nigrofuscata (Homoptera) : analysis of its primary structure Insect Biochem"Molec.Biol.. 30. 1-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee, J.M., M.Hatakeyama & K.Oishi: "A simple and rapid method for cloning insect vitellogenin cDNAs."Insect Biochem.Molec.Biol.. 30. 189-194 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liang, B.-L.& Y.Fuyama: "Can D.simulans breed on Morinda citrifolia, the host plant of D.sechellia?"Dros.Inform.Serv.. (in press)83. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomaru, M., M.Doi, H.Higuchi & Y.Oguma: "Courtship song recognition in the Drosophila melanogaster complex : Heterospecific songs make females receptive in Drosophila melanogaster but not in D."sechellia.Evolution. 54. 1286-1294 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomaru, M.& Y.Oguma: "Mate choice in Drosophila melanogaster and D.sechellia : criteria and their variation depending on courtship song"Anim.Behav.. 60. 797-804 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tufail, M., J.M.Lee, M.Hatakeyama, K.Oishi & M.Takeda: "Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analysis."Archiv.Insect Biochem.Physiol.. 45. 37-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Araki, H., N.Inomata & T.Yamazaki: "Molecular evolution of duplicated amylase gene regions in Drosophila melanogaster : Evidence of positive selection in the coding regions and selective constraint in the cis-regulatory regions"Genetics. 157. 667-677 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Doi, M., M.Matsuda, M.Tomaru, H.Matsubayashi & Y.Oguma: "A locus for female discrimination behavior causing sexual isolation in Drosophila"Proc.Natl.Acad.Sci.USA. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii, K., Y.Hirai, C.Kafagiri & M.T.Kimura: "Sexual isolation and cuticular hydrocarbons in Drosophila elegansl Heredity"(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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