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2000 Fiscal Year Final Research Report Summary

Analysis of polysomaty in plants and its evaluation for genetics and plant breeding

Research Project

Project/Area Number 10306001
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

MII Masahiro  Professor, Faculty of Horticulture, Chiba University, 園芸学部, 教授 (30093074)

Co-Investigator(Kenkyū-buntansha) OHMI Keiko  Assistant Professor, , Graduate School of Natural Sciences, Chiba University, 大学院・自然科学研究科, 助手 (20212839)
NAKAMURA Ikuo  Associate Professor., Graduate School of Natural Sciences, Chiba University, 大学院・自然科学研究科, 助教授 (50207867)
Project Period (FY) 1998 – 2000
Keywordspolysomaty / flow cytometry / chromosome doubling / plant taxonomy / DNA content / analysis of hybridity / somaclonal variation / polyploid production
Research Abstract

Flowcytometric analysis was conducted on the distribution of polysomaty in economically important plants and common features of the plants with polysomaty were investigated based on the data of the analysis. As the results, polysomaty was widely found in the plants with succulent organs, but it was also found in some non-succulent plants belonging to some taxons such as Caryophyllales. Moreover, woody plants rarely have polysomaty. These results suggest that polysomaty have been acquired independently at different aspects of evolutionary processes. Polysomaty was found in all organs except for shoot and root apical meristems and the cells with higher ploidy levels tended to increase with aging. In the artificially induced tetraploids, polysomaty pattern shifted to the one-step higher level but the proportion of the highest ploidy cells tended to decrease. Relationship between polysomaty and the spontaneous production of polyploids as a somaclonal variation during the micropropagation of plants was also examined. As a result, cell suspension culture induced from the tissue with polysomaty had almost the same pattern of polysomaty as the original tissue, suggesting that the cells with higher ploidy levels had the same ability for cell division as the basal diploid cells. Since the polysomaty pattern changed by the kind and concentration of plant growth regulators added to the culture medium, plant growth regulators are considered to be a major factor for the polyploidization of the cultured cells. In the present stydy, it was revealed that DNA content is a useful index for discriminating the different taxons, . Moreover, flow cytometric analysis of DNA contents was effective for confirming the hybridity of the plants obtained by the cross between the species which have different DNA contents, and for identifying the maternal parent of the interspecific polyploid hybrid produced by the cross between the species with the same nature.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Supaibulwatana,K.: "Induction of meristematic nodular calli from various explants of Lilium Spp. and long term stability in plant regeneration ability and ploidy level of the calli."Plant Biotechnology. Vol.15. 95-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aziz-Purwantoro: "Cytological and RAPD (Random Amplified Polymorphic DNA) analyses of somaclonal variation in Easter lily (Lilium longiflorum Thunb.)"Plant Biotechnology. Vol.16. 247-250 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mishiba: "Nuclear DNA content as an index character discriminating taxa in the genus Petunia sensus Jussieu (Solanaceae)"Annals of Botany. Vol.85. 665-673 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mishiba: "Polysomaty analysis in diploid and tetraploid Portulaca grandiflora"Plant Science. 156. 213-219 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nozaki, T.: "Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B.campestris"Theoretical and Applied Genetics. Vol.101. 538-546 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,J.: "Differences in ploidy levels of inter-specific hybrids obtained by reciprocal crosses between Primula sieboldii and P.kisoana."Theoretical and Applied Genetics. Vol.101. 690-696 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Godo,T.: "In vitro culture for preservation of triploid Senno (Lychnis senno Siebold et Zucc., 2n=36), avaluable and rare omamental plant."Bull.Bot.Gard.Toyama. Vol.5. 35-43 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,J.: "Different genomic combinations in inter-section hybrids obtained from the crosses between Primula sieboldii (Section Cortusoides) and P. obconica (Section Obconicolisteri) by the embryo rescue technique"Theoretical and Applied Genetics. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mishiba,K.: "Increasing ploidy level in cell suspension cultures of Doritaenopsis by exogenous application of 2,4-dichlorophenoxyacetic acid."Physiologia Plantarum. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa: "Tetraploid bell pepper shows high in vitro pollen germination at 15℃"HortScience. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三柴啓一郎: "フローサイトメトリー自由自在.中内啓光監修,(細胞工学別冊)"秀潤社. 101(7) (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Supaibulwatana, K.and M.Mii: "Induction of meristematic nodular calli from various explants of Lilium spp. and long term stability in plant regeneration ability and ploidy level of the calli."Plant Biotechnol.. 15. 95-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aziz-Purwantoro, K.Supaibulwatana, M.Mii and T.Koba: "Cytological and RAPD (Random Amplified Polymorphic DNA) analyses of somaclonal variation in Easter lily (Lilium longiflorum Thunb.)."Plant Biotechnol.. 16. 247-250 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mishiba, K., T.Ando, M.Mii, H.Watanabe, H.Kokubun, G.Hashimoto, E.Marchesi: "Nuclear DNA content as an index character discriminating taxa in the genus Petunia sensus Jussieu (Solanaceae)."Ann.Bot.. 85. 665-673 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mishiba, K.and M.Mii: "Polysomaty analysis in diploid and tetraploid Portulaca grandiflora."Plant Sci.. 156. 213-219 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nozaki, T., K.Mishiba, M.Mii and T.Koba: "Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B.campestris."Theor.Appl.Genet. 101. 538-546 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, J.and M.Mii: "Differences in ploidy levels of inter-specific hybrids obtained by reciprocal crosses between Primula sieboldii and P.kisoana."Theor.Appl.Genet. 101(5). 690-696 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Godo, T., T.Oku, Mii, M.and M.Nakata: "In vitro culture for preservation of triploid Senno (Lychnis senno Siebold et Zucc., 2n=36), avaluable and rare ornamental plant."Bull.Bot.Gard.Toyama. 5. 35-43 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, J., R.Ishikawa and M.Mii: "Different genomic combinations in inter-section hybrids obtained from the crosses between Primula sieboldii (Section Cortusoides) and P.obconica (Section Obconicolisteri) by the embryo rescue technique."Theor.Appl.Genet.. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mishiba, K.and M.Mii: "Increasing ploidy level in cell suspension cultures of Doritaenopsis by exogenous application of 2, 4-dichlorophenoxyacetic acid."Physiol.Plant.. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, K., H.Kuboki, K.Mishiba and O.Nunomura: "Tetraploid bell pepper shows high in vitro pollen germination at 15℃."HortSci.. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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