2000 Fiscal Year Final Research Report Summary
Studies on a mechanism of hantavirus persistent infection in central nervous system and immune system
| Project/Area Number |
10306019
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARIKAWA Jiro Hokkaido University school of Medicine Professor, 医学部, 教授 (10142704)
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| Co-Investigator(Kenkyū-buntansha) |
MORIKAWA Shigeru National Institute of Infectious Disease Section of Virology Chief, ウイルス第一部外来性ウイルス室, 室長(研究職) (00167686)
KARIWA Hiroaki Hokkaido University Graduate School of Veterinary Medicine Associate Professor, 大学院・獣医学研究科, 助教授 (70224714)
MORIMATSU Kumiko (吉松 組子) Hokkaido University School of Medicine Instructor, 医学部, 助手 (90220722)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Hemorrhagic fever with renal syndrome / HFRS / hantavirus / zoonosis / virus infection / pathogenicity / genetic reassortant / infection enhancement |
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| Research Abstract |
1. Hantavirus infection was enhanced about 10 times by addition of lectins (DBA and SBA) which specifically bind to N-acetylgalactosamine. This enhancement was considered to caused by the cross linking between receptor to virion by the lectins.
2. Two Vero E6 cell subclones, one induce low pH dependent cell fusion after hantavirus infection and the other resistant to cause cell fusion, were established. By using the two cell clones, the possible role for the cellular factor for responsible for induce infected cell fusion was considered.
3. After peripheral infection of hantavirus to mice, virulent virus reached to brain 4 to 5 days earlier than that of avirulent virus. This difference was considered as a mechanism which define the virulence of hantavirus in the mouse model.
4. Virulent hantavirus showed higher growth rate in the brain micro vascular cells and peritoneal macrophage, suggesting that virulence relates to the growth ability in the target organs.
5. Genetic reassortant virus between virulent and avirulent viruses were established. The comparison of the virulence of the reassortant viruses indicated that one amino acid difference at enveloped protein and nucleotide difference in the polymerase gene are related to the virulence.
6. Virulent hantavirus infected SCID mice were passively transferred with spleen cells of immunized BALB/c mice 3 weeks after infection. The recipient SCID mice represented apparent weight loss 4 days after the transfer, indicating the immune mediated pathogenicity.
7. Hantaan virus S and M genome segment which encoding nucleocapsid protein and enveloped glycoprotein, respectively were cloned and expressed in the mammalian cells.
8. Entire L genome segment which encodes polymerase was cloned. The expression of the L clone is under going.
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