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2000 Fiscal Year Final Research Report Summary

Bioremediation of Soil pollutants by Plant-Microbe Symbiotic System

Research Project

Project/Area Number 10306021
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源科学
Research InstitutionRIKEN

Principal Investigator

YAMAGUCHI Isamu  RIKEN, Microbial Toxicology Laboratory, Chief Scientist, 微生物制御研究室, 主任研究員 (20087589)

Co-Investigator(Kenkyū-buntansha) MOTOYAMA Takayuki  RIKEN, Microbial Toxicology Laboratory, Researcher, 微生物制御研究室, 研究員 (70291094)
ARIE Tsutomu  RIKEN, Mocrobiology Laboratory, Senior Research Scientist(1998-1999) ; Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate professor(2000), 農学部, 助教授 (00211706)
KUDO Toshiaki  RIKEN, Mocrobiology Laboratory, Chief Scientist, 微生物学研究室, 主任研究員 (80109793)
Project Period (FY) 1998 – 2000
KeywordsPollutants / Bioremediation / Phytoremediation / Plant-Microbe Symbiotic System / Rhizosphere microorganisms / PCNB / PCP / PCP
Research Abstract

1) Biodegradation of PCNB and its Derivatives in Soil by Plant-Microbe Symbiotic System
Rhizosphere microorganisms were subjected to screening for ability to degrade PCNB, a fungicide used to control clubroot disease of Brassica spp., and its derivatives. Pseudomonas gladioli M-2196, one of those microbes that metabolize PCNB to PCA, was a symbiont on the root of Allium spp. Mortierella sp. 15-4, a fungal symbiont on the root of Brassica spp., could degrade PCNB into PCTA.The M-2196-Allium spp. and 15-4-Brassica spp. symbiotic models showed effective degradation of PCNB in soil into PCA and PCTA, respectively. A gene(pcpB)encoding a PCP degrading enzyme was cloned from Sphingomonas chloropheolica and used to transform P.gladioli M-2196 and Fusarium sp. F4, both of which are plant symbionts. The transformed microbe-plant symbiotic systems could degrade PCP in soil effectively. We also found out the biocontrol ability in Phoma glomerara on clubroot and suggested that the effect was due to epoxydon produced by the fungus.
2) Accumulation of Metals by Plant from Soil using Plant-Microbe Symbiotic System
Several plant species showed different ability in accumulating metals from soil. Environmental factors, such as soil types and plant-symbiotic microbes gave effects on the accumulation, i. e., Fusarium sp. T3 increased the uptake of Cs and Sr by tomato.
3) Molecular-Evolutional Analysis on Symbiosis
Strategies for transformation of plant-symbiotic microbes were examined and symbiosis-deficient mutants were obtained by restriction enzyme mediated integration (REMI)method. A gene that might have a sole in symbiosis was identified from plant-symbiotic Fusarium sp. Futher, molecular analysis was performed on the evolution of plantsymbiotic microbes.

  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] T. Arie el al. : "Control of soilborne clubroot disease of cruciferous plants by epoxydon from Phoma glomerata"Plant Pathology . 47. 743-748 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Gouthu et al.: "Subcellular distribution and translocation of radionuclides in plants"Environ. Toxicol. Chem. . 18・9. 2023-2027 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Gouthu: "Possible role of soil-microbes in plant uptake of radionuclides"RIKEN Accel. Prog. Rept. . 31. 146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Arai et al.: "Adaptation of Comamonas yestosteroni TA441 to utilize phenol : organization and regulation of the genes involved in phenol degradation"Microbiology. 146. 1707-1715 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie el al.: "Control of clubroot of cruciferous by Phoma glomerata and its product epoxydon "Pestic. Sci.. 55・5. 602-604 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有江 力: "放射性核種の土壌から植物への移行に関する根圏土壌ファクター"JAERI-Conf. 99・001. 202-212 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Gouthu et al.: "Translocation behavior of Sr, Rb, and Zn in soybean"RIKEN Accel. Prog. Rept.. 32. 142-143 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie et al.: "Phoma glomerata and its product epoxydon supress clubroot"Tohoku National Agricultural Experiment Station. 15. 30-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Arai et al.: "Genetic organization and characteristics of the 3-(3-hydroxyphenyl) propionic acid degradation pathway of Comamonas testosteroni TA441"Microbiology. 145. 2813-2820 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Suzuki et al.: "Haematocin, a new antifungal diketopiperazine produced by Nectria haematococca causing Nectria blight disease of ornamental plants"J. Antibiot.. 53・1. 311-314 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Arai et al.: "Adaptation of Comamonas testosteroni TA441 to utilization of phenol by spontaneous mutation of the gene for a trans-acting factor"Molecular Microbiology. 33・6. 1132-1140 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie et al. : "Mating-type genes from asexual phytopathogenic ascomycetes Fusarium oxysporum and Alternaria alternata"Mol. Plant-Microbe Interact.. 13・12. 1330-1339 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. -H Yun et al.: "Mollecular organization of mating type loci in heterothallic, homothallic, and asexual Gibberellal Fusarium species"Fungal Genet. Biol.. 31・1. 7-20 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Fujiwara et al.: "Evidence that the Asprgillus nidulans class I and class II chitin synthase genes, chsC and chsA, share critical roles in hyphal wall integrity and conidiophore development"J. Biochem. 127. 359-366 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有江 力: "食用植物の保護技術と環境問題"環境科学総合研究所年報. 19. 5-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口 勇: "陸域環境における放射性核種の移行に関する動的モデルの開発"理化学研究所. 23 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie et al.: "Proceedings for the 9th Japan-China Symposium on pesticide science"An environmentally compatible approach to control soilborne disease : suppression of cruciferous clubroot by Phoma glomerata and epoxydon. 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有江 力: "農薬バイオテクノロジー関連技術調査報告書"生物農薬研究の現状と展望. 13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie et al.: "Proceedings of Internatiional Conference on Asian Network on Microbial Researchers"Biodegradation of pentachloronitrobenzene (PCNB) by rhizo-microbes. 9 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立沢 智 ら: "日本農薬学会第25回大会要旨集"植物根圏微生物のペンタクロロニトロベンゼン代謝能. 1(139) (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Arie et al.: "Agrochemical diversity"Trends in biocontrol research in soilborne plant diseases : in Integrated management progtams on clubroot disease of cruciferous. 10(317) (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arai, H., Akahira, S., Ohishi, T., Maeda, M.and Kudo. T: "Adaptation of Comamonas testosteroni TA441 to utilize phenol : organization and regulation of the genus involved in phenol degradation"Microbiology. 144. 2895-2903 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai, H., Akahira, S., Ohishi, T., and Kudo, T: "Adaptation of Comamonas testosteroni TA441 to utilization of phenol by spontaneous mutation of the gene for trans-acting factor"Molecular Microbiology. 33. 1132-1140 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai, H., Ohishi, T., Chang, M.-Y.and Kudo, T: "Arrangement and regulation of the genes for a meta-pathway enzymes required for degradation of phenol in Comamonas testosteroni TA441"Microbiology. 146. 1707-1715 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arai, H., Yamamoto, T.Ohishi, T., Shimizu, T., Nakata, T.and Kudo, T: "Genetic organization and characterization of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441"Microbiology. 145. 2813-2820 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kobayashi, Y., Okada, G., Kono Y.and Yamaguchi, I.: "Control of soilborne clubroot disease of crucifeous plants by epoxydon from Phoma glomerata"Plant Pathology. 47. 743-748 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kobayashi, Y., Kono Y., Okada, G., and Yamaguchi, I.: "Control of clubroot of cruciferous by Phoma glomerata and its product epoxydon"Pestic. Sci. 55. 602-604 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Yoshida, T., Shimizu, T., Kawabe, M., Yoneyama, K.and Yamaguchi, I.: "Assessment of Gibberella fujikuroi mating type by PCR."Mycoscience. 40. 311-314 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kaneko, I., Yoshida, T., Noguchi, M., Nomura, Y.and Yamaguchi, I.: "Mating type genes from asexual phytopathogenic ascomyvetes"Fusarium oxysporum and Alternaria alternata MPMI. 13. 1300-1339 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara, M., Ichinomiya, M., Motoyama, T., Horiuchi, H., Ohta, A.and Takagi, M.: "Evicence that the Aspergillus nidulans class I and class II chitin synthase genes, chsC and chsA, share critval roles in hyphal wall integrity and conidiophore development"J.Biochem. 127. 359-366 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Weginwar, R., Arie, T., Ambe, S., Ozaki, T., Enomoto, S., Ambe, F.and Yamaguchi, I: "Subcellular distribution and translocation of radionuclides in plants."Environ. Toxical. Chem. 18. 2023-2027 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, Y., Takahashi, H., Esumi, Y., Arie, T., Morita, T., Koshino, H., Uzawa, J., Uramoto. M.and Yamaguchi, I: "Haematocin, a new antifungal diketopiperazine produced by Nectria haematococca Berk. et Br.(880701a-1)causing Nectria bright disease on omamental plants"J.Antibiot. 53. 45-49 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yun, S.-H., Arie, T., Kaneko, I., Yoder, O.C., Turgeon, B.G.: "Molecular organization of mating type loci in heterothallic, homothallic and asexual Gibberellal Fusarium species"Fungal Genet. Biol. 31. 7-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kobayashi, Y.and Yamaguchi, I: "An environmentally conpatible approach to control soilborne disease -Suppression of cruciferous clubroot by Phoma glomerata and its product epoxydon"Proceedings for the 9th Japan-China Symposium on Pesticide Science, Nankai University, Tianjin, China. 30-59 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Motoyama, T., Tatsusawa, S., Kadokura, K., Yamazaki, N., Okada, G., and Yamaguchi, I: "Biodegradation of pentachloronitrobenzene (PCNB)by rhizo-microbes : Proceeding of International Conference on Asian Network on Microbial Researches"Thailand Institute of Scientific and Technological Research. 287-295 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kobayashi, Y.and Yamaguchi, I: "Phomo glomerata and its product epoxydon suppress clubroot."Tohoku National Agricultural Experiment Starion(A). 15. 30-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arie, T., Kobayashi, Y.and Yamaguchi, I: "Trends in Biocontrol Research on Solibone Plant Diseases : Integrated Management Programs on Clubroot Disease of Cruciterous : Agrochemical Discovety"American Chemical Society, Washington D.C., USA. 142-151 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Arie, T., Ambe, S.and Yamaguchi, I.: "Screening of plants for soil-radionuclide accumulators."RIKEN Accel. Prog. Rep.. 31. 145 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Arie, T., Ambe, S.and Yamaguchi, I.: "Possible role of soil-microbes in plant uptake of radionuclides"RIKEN Accel Prog. Rep. 31. 146 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Arie, T.and Yamaguchi, I.: "Uptake of ^<137>Cs by and ^<90>Sr by cucumber in three soil-types : Comparative Evaluation of Environmental Toxicants"National Institute of Radiological Science Chiba, Japan. 275-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Arie, T.and Yamaguchi, I.: "Uptake of ^<137>Cs by and ^<90>Sr by cucumber as affected by soil and microbial paramenters of three soil-types : Proceeding of International Workshop on Comparative Evaluation of Health Effects of Environmental Toxicants derived from Advanced Technologiesn three soil-types : Comparative Evaluation of Environmental Toxicants derived from Advanced Technology"National Institute of Radiological Science, Chiba, Japan. 79-87 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gouthu, S., Arie, T., and Yamaguchi, I.: "Translocation behavior of Sr, Rd. and Zn in soybean"RIKEN Accel Prog. Rep. 232. 142-143 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Soshi, T., Arie, T., and Yamaguchi, I.: "Influence of no-pathogenic Fusarium on uptake of radionuclides by tomato"RIKEN Accel Prog. Rep. 33(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, I., Nakashita, H., Arie, T., Kobayashi. Y., Someya. K., Tamura, K., Kono, Y.and Okada, G.: "Microbial degradation of PCNB, a residual fungicide against clubroot : How can clubroot disease of crucifeers be controlled with less impact on the environment? in Asian Network on Microbial Researches"Proceedings of International Conference on Asian Network on Microbial Researches, Gadjah Mada University, RIKIN and STA. 359-370 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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