1998 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Research Project

Project/Area Number	10307053
Research Category	Grant-in-Aid for Scientific Research (A)
Research Institution	Kyushu University
Principal Investigator	前田勝正九州大学, 歯学部, 教授 (00117243)
Co-Investigator(Kenkyū-buntansha)	平田雅人九州大学, 歯学部, 教授 (60136471) 石川烈東京医科歯科大学, 歯学部, 教授 (10014151) 吉野宏広島大学, 歯学部, 助手 (50240338) 吉江弘正新潟大学, 歯学部, 助教授 (20143787) 根本英二東北大学, 歯学部, 助手 (40292221)
Keywords	歯周病 / リポ多糖 / 好中球 / PGE2 / ヒアルロン酸 / 歯根膜 / 生体防御 / 組織修復
Research Abstract	1. 歯周病における生体防御反応の解析細菌の有する重要な毒性物質であるリポ多糖(LPS)に対して好中球は、歯面などに付着した状態の場合には、LPS結合蛋白質が存在しなくてもフィブロネクチンなどがあれば応答することが明かとなった。また、このLPSに対する線維芽細胞の応答にはCD14分子が重要であることが明かとなった。歯周炎の再発患者では、細胞表面のFcγレセプターの遺伝子表現型が158Vが多い事が明かとなった。また、歯周病細菌に対する炎症局所の免疫応答を解析したところ、特異抗体の産生とともに特異性の低い抗体が産生されていることが明かとなった。さらに、炎症性細胞の機能発現に関する研究や疾患の発現に関する免疫応答とその制御の解析についても重要な研究結果が得られている。(発表論文参照) 2. 歯周病における組織修復の解析組織修復についての動物実験モデルを使った解析により以下のことが明かとなっている。 1) PGE_2投与によって歯槽骨吸収の抑制が示唆されている。 2) 組織修復に伴いヒアルロン酸の増加が観察されるとともに特異遺伝子の存在が観察されている。 3) 増殖した歯根膜組織を移植した群では、骨の新生とともに一定の幅の歯根膜組織の形成が認められた。以上、歯周病における生体防御反応及び組織修復について重要な研究成果を得ており、今後これらの関連性についても検討を行う予定である。

Research Products
(15 results)

All Other

All Publications (15 results)

[Publications] Kensuke Nakatomi: "Neutrophils responded to immobilized lipopolysaccharide in the absence of lopopolysaccharide-binding protein" Journal of Leukocyte Biology. 64. 177-184 (1998)
[Publications] Shunji Sugawara: "Heterogeneous expression and release of CD14 by human gingival fibroblasts:characterization and CD14-mediated interleukin-8 secretion in response to lipopolysaccharide" Infection and Immunity. 66. 3043-3049 (1998)
[Publications] Joichiro Hayashi: "Increased Soluble CD14 Serum Levels in Periodontitis Patients" Infection and Immunity. 67. 417-420 (1999)
[Publications] K.Noguchi: "Interleukin-4 and interferon-gamma,inhibit prostaglandin production by interleukin-lbeta-stimulated human periodonal" Inflammation. 23(in press). (1999)
[Publications] Aramaki M.: "Presence of activated B-1 cells in chronic inflamed gingival tissue" J Clin Immunol. 18(6). 421-429 (1998)
[Publications] Hirata,M.: "Pleckstrin homoplogy domain as an inositol compound binding" Jap.J.Pharmacol.76. 255-263 (1998)
[Publications] Yagisawa,H.: "Replacements of single basic amino acids in the pleckstrin homology domain of phospholipase C-δ1 alter the ligand binding,phos-pholipase activity and interaction with the plasma membrane" J.Biol.Chem.273. 417-424 (1998)
[Publications] Takeuchi,H.: "PTB domain of insulin receptor substrate-1 binds inositol compounds" Biochem.J.334. 211-218 (1998)
[Publications] Masahiro Ohguchi: "Activin A Regulates the Production of Mature Interleukin-1β and Interleukin-1 Receptor Antagonist in Human Monocytic Cells" Journal of Interferon and Cytokine Research. 18. 491-498 (1998)
[Publications] Yomomi Watanabe-Kato: "Isolation and characterization of transposon-induced mutants of porphyromnas gingivalis deficient in fimbriation" Microbial Pathogenesis. 24. 25-35 (1998)
[Publications] Masahiro Ohguchi: "Actinobacillus actinomycetemcomitans Toxin induces both Cell cycle arrest in the G2/M Phase and Apoptosis" Infection and Immunity. 66. 5980-5987 (1998)
[Publications] Fukui Y.: "Highly restricted T cell repertoire shaped by a single major histocompatibility complex-peptide ligandin the presence of a single rearrang-ed T cell receptorβ chain" J.Exp.Med.188. 897-97 (1998)
[Publications] Gapin L.: "Quantitative analysis of the T-cell repertoire by a single petide/MHC complex" J.Exp.Med.187. 1871-1883 (1998)
[Publications] Tana T.: "A HLA binding motif-aided peptide epitope library:A novel library desgin for the screening of HLA-DR4-restricted antigenic peptides recognized by CD4^+T cells" J.Human Genet.43. 14-21 (1998)
[Publications] Nakno K.: "Prostaglandin E_2 inhibits alveolar bone resorption in experimental periodontitis in hamsters" Dentistry in Japan. 34. 108-111 (1998)

1998 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Principal Investigator

前田 勝正 九州大学, 歯学部, 教授 (00117243)

Research Products

[Publications] Kensuke Nakatomi: "Neutrophils responded to immobilized lipopolysaccharide in the absence of lopopolysaccharide-binding protein" Journal of Leukocyte Biology. 64. 177-184 (1998)

[Publications] Shunji Sugawara: "Heterogeneous expression and release of CD14 by human gingival fibroblasts:characterization and CD14-mediated interleukin-8 secretion in response to lipopolysaccharide" Infection and Immunity. 66. 3043-3049 (1998)

[Publications] Joichiro Hayashi: "Increased Soluble CD14 Serum Levels in Periodontitis Patients" Infection and Immunity. 67. 417-420 (1999)

[Publications] K.Noguchi: "Interleukin-4 and interferon-gamma,inhibit prostaglandin production by interleukin-lbeta-stimulated human periodonal" Inflammation. 23(in press). (1999)

[Publications] Aramaki M.: "Presence of activated B-1 cells in chronic inflamed gingival tissue" J Clin Immunol. 18(6). 421-429 (1998)

[Publications] Hirata,M.: "Pleckstrin homoplogy domain as an inositol compound binding" Jap.J.Pharmacol.76. 255-263 (1998)

[Publications] Yagisawa,H.: "Replacements of single basic amino acids in the pleckstrin homology domain of phospholipase C-δ1 alter the ligand binding,phos-pholipase activity and interaction with the plasma membrane" J.Biol.Chem.273. 417-424 (1998)

[Publications] Takeuchi,H.: "PTB domain of insulin receptor substrate-1 binds inositol compounds" Biochem.J.334. 211-218 (1998)

[Publications] Masahiro Ohguchi: "Activin A Regulates the Production of Mature Interleukin-1β and Interleukin-1 Receptor Antagonist in Human Monocytic Cells" Journal of Interferon and Cytokine Research. 18. 491-498 (1998)

[Publications] Yomomi Watanabe-Kato: "Isolation and characterization of transposon-induced mutants of porphyromnas gingivalis deficient in fimbriation" Microbial Pathogenesis. 24. 25-35 (1998)

[Publications] Masahiro Ohguchi: "Actinobacillus actinomycetemcomitans Toxin induces both Cell cycle arrest in the G2/M Phase and Apoptosis" Infection and Immunity. 66. 5980-5987 (1998)

[Publications] Fukui Y.: "Highly restricted T cell repertoire shaped by a single major histocompatibility complex-peptide ligandin the presence of a single rearrang-ed T cell receptorβ chain" J.Exp.Med.188. 897-97 (1998)

[Publications] Gapin L.: "Quantitative analysis of the T-cell repertoire by a single petide/MHC complex" J.Exp.Med.187. 1871-1883 (1998)

[Publications] Tana T.: "A HLA binding motif-aided peptide epitope library:A novel library desgin for the screening of HLA-DR4-restricted antigenic peptides recognized by CD4^+T cells" J.Human Genet.43. 14-21 (1998)

[Publications] Nakno K.: "Prostaglandin E_2 inhibits alveolar bone resorption in experimental periodontitis in hamsters" Dentistry in Japan. 34. 108-111 (1998)

前田勝正九州大学, 歯学部, 教授 (00117243)