1999 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Research Project

Project/Area Number	10307053
Research Institution	Kyushu University
Principal Investigator	前田勝正九州大学, 歯学部, 教授 (00117243)
Co-Investigator(Kenkyū-buntansha)	吉野宏広島大学, 歯学部, 助手 (50240338) 吉江弘正新潟大学, 歯学部, 教授 (20143787) 根本英二東北大学, 歯学部, 助手 (40292221) 平田雅人九州大学, 歯学部, 教授 (60136471) 石川烈東京医科歯科大学, 歯学部, 教授 (10014151)
Keywords	リポ多糖 / インターロイキン / 好中球 / 線維芽細胞 / エムドゲイン / テトラサイクリン / プロスタグランディン / 再生の遺伝子
Research Abstract	1.歯周病における生体防御反応の解析:歯周病関連細菌のリポ多糖(LPS)の好中球及び単球に対する活性化機序の解析によるとP.gingivalisとA.actinomycetemcomitansとでは活性化に関与するレセプターに差異がある事が明かとなった。好中球のFcレセプターの遺伝子解析によると貧食能、活性酸素産生能の低下しているNA2/NA2群の方が歯周病に罹患し易い事が明らかとなった。線維芽細胞は、インターロイキン1(IL-1)やLPSの刺激により種々の炎症性ケモカインの活性を制御するCD26/Dipeptidyl peptidase IVを発現する事が明らかになった。更に、線維芽細胞はIL-1やTGF-β、PDGFなどの細胞増殖因子の刺激により高分子量のヒアルロン酸を産生し炎症を制御している事が観察された。又唾液中の分泌型IgA抗体量と歯周病の臨床症状との間に関連性が認められた。更に、炎症性細胞の機能発現に関与するIP3結合タンパク質の解析や抗体産生応答性についてのT細胞サブセットの解析についても重要な研究結果が得られている。 2.歯周病における組織修復の解析:歯周組織再生療法に臨床使用されているエムドゲインを応用とするとアルカリフォスファターゼ陽性の歯根膜細胞の増殖を促進した。又ヒト歯根膜由来線維芽細胞のSPARK分子の発現が歯周組織の破壊の進行に関与している事が示唆された。歯槽骨の吸収及び再生を動物実験モデルにて解析したところプロスタグランディンE_2(PGE_2)の全身投与を行うと歯周炎局所での骨吸収の抑制が観察されるとともにテトラサイクリン併用の組織再生誘導(GTR)法を行うと新生歯槽骨の形成が促進された。更に、ラット歯槽骨の創傷治癒過程で発現量が変化する遺伝子群の検索を行い興味深い知見を得ている。以上、歯周病における生体防御反応及び組織修復について重要な研究成果を得ており、今後これらの研究を更に発展させると共に臨床応用についての可能性の検討を行う予定である。

Research Products
(27 results)

All Other

All Publications (27 results)

[Publications] Yukiko Abe: "Development of Mineralized Nodules in Fetal Rat Mandibular Osteogenic Precursor Cell:Reguirement for Dexamethasone But not for β-qlycerophosphate"Caclcified Tissue International. 66(1). 66-69 (2000)
[Publications] Akiko Matsumoto: "An immunohistochemical study of the behavior of cellos exoressing IL-1α and IL-1β within experimentally induced periapical lesions in rats"Journal of Endodontics. 24(12). 811-816 (1998)
[Publications] Eiji Nemoto: "Increase of CD26/Dipeptidyl peptidase IV expression on human gingival fibroblasts upon stimulation with cytokines and bacerial components"Infection and Immunity. 67. 6225-6233 (1999)
[Publications] T.Kobayashi: "Relevance of IoG receptor IIIb(CD16) polymorphism to handing of porphiromonas gingivalis:implications for the pathogenesis of adult periodontitis"Journal of Periodontal Research. 35(1). 1-9 (2000)
[Publications] N.Sugita: "Relevance of Fcγ RIIIa-158V-F polymorphism to recurrence of adult peiodontitis in Japanese patients"Clinical and Experimental Immunology. 117(2). 350-354 (1999)
[Publications] 村上伸也: "ヒアルロン酸の生物学的活性を考える"炎症(日本炎症学会雑誌). 19(6). 307-318 (1999)
[Publications] Nagasawa T.: "The role of salivary IgA antibody against periodontopathic bacteria"Oral Diseases. 5. 63-65 (1999)
[Publications] Ono,Nagasawa T.: "Periodontal status of selective IgA deficiency in a juvenile:case report"Journal of the International Academy of Periodontology. 1. 16-20 (1999)
[Publications] T.Nagasawa: "Oral administration of Porphromonas gingvalis fimbriae with cholera toxin induces antifibriae serum IgG,IgM,IgA and salivary IgA antibodies"Journal of Periodontal Research. 33. 169-174 (1999)
[Publications] Hirata M.: "Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding"Mol.Cell.Biochem.. 190. 179-184 (1999)
[Publications] Yoshimura K.: "Use of phosphorofluoridate analogues of D-myo-inositol 1,4,5-trisphosphate to assess the involvement of ionic interactions in its recognition by the receptor and metabolising enzymes"Cell.Signal.. 11. 117-125 (1999)
[Publications] Caffrey,J.J.: "Multiple inositol polyphosphate phosphatase:Molecular cloning of the human form of an enzyme in endoplasmic reticulum that is upregulated during chondrocyte hypertrophy"FEBS Lett.. 442. 99-104 (1999)
[Publications] Takeuchi H.: "Membrane association of a new inositol 1,4,5-trisphosphate binding protein,p130 is not dependent on the pleckstrin homology domain"Chem.Phys.Lipids. 98. 35-47 (1999)
[Publications] Matsuki N.: "Antibodies against the PH domain of phospholipase C-1 inhibit Ins (1,4,5) P3-mediated Ca2^+ release from the endoplasmic reticulum"Biochem.Biophys.Res.Commun.. 260. 42-47 (1999)
[Publications] Yamamoto T.: "Involvement of EF hand motifs in the Ca2^+-dependent binding of the pleckstrin homology domain to phosphoinositides"Eur.J.Biochem.. 265. 481-490 (1999)
[Publications] Savoie CJ.: "The peptide binding motif of HLA-A^*0217"Immunogenetics. 49. 567-570 (1999)
[Publications] Matsuki N.: "Prevention of infection of influenza virus in DO6 mice,a human model,by a peptide vaccine prepared according to the cassette theory"Vaccine. 17. 1161-1168 (1999)
[Publications] Yoshitake S.: "HLA class II alleles in Japanese patients with inflammatory bowel disease"Tissue Antigens. 53. 350-358 (1999)
[Publications] Sano T.: "Spontaneous clustering of Thy-1 antigens on CD4^+ CD8^+ thymocytes lacking TCR engagement by MHC/peptode complexes"Eur.J.Immunol.. 29. 403-412 (1999)
[Publications] Nishimura H.: "MHC class II-dependent NK1.1^+ T cells are induced in mice by Salmonella infection"J.Immunol.. 162. 1573-1581 (1999)
[Publications] Nakano K.: "Prostaglandin E_2 inhibits alveolar bone resorption in experimental periodontitis in hamsters"Dentistry in Japan. 34. 108-111 (1998)
[Publications] Sawada K.: "Indentification of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis by subtractive hybridization"Infection and Immunity. 67. 5621-5625 (1999)
[Publications] 高柴正悟: "歯髄組織の創傷治癒時に特徴的に発現する遺伝子"歯科保存学雑誌. 42春季特別. 17-17 (1999)
[Publications] 大平泰資: "歯周組織創傷治癒過程に発現する遺伝子の同定"日本歯周病学会会誌. 41秋季特別. 117-117 (1999)
[Publications] Yamamoto T.: "Unique genes expressed in fibroblasts from human periodontal ligament in vitro"Program and Abstracts (11th International Conference on Periodontal Research). 18-18 (1999)
[Publications] Shogo Takashiba: "Concept for biological treatment of Periodontal disease"Program and Abstracts (3rd Asian Pacific Society of Periodontology). 20-20 (1999)
[Publications] 島田顕: "組織培養による抜去歯の残存歯根膜からルートプレーニングした根面への細胞増殖とアルカリフォスファターゼ活性発現に関する研究"歯科保存学雑誌. 43(1). 254-261 (2000)

1999 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Principal Investigator

前田 勝正 九州大学, 歯学部, 教授 (00117243)

Research Products

[Publications] Yukiko Abe: "Development of Mineralized Nodules in Fetal Rat Mandibular Osteogenic Precursor Cell:Reguirement for Dexamethasone But not for β-qlycerophosphate"Caclcified Tissue International. 66(1). 66-69 (2000)

[Publications] Akiko Matsumoto: "An immunohistochemical study of the behavior of cellos exoressing IL-1α and IL-1β within experimentally induced periapical lesions in rats"Journal of Endodontics. 24(12). 811-816 (1998)

[Publications] Eiji Nemoto: "Increase of CD26/Dipeptidyl peptidase IV expression on human gingival fibroblasts upon stimulation with cytokines and bacerial components"Infection and Immunity. 67. 6225-6233 (1999)

[Publications] T.Kobayashi: "Relevance of IoG receptor IIIb(CD16) polymorphism to handing of porphiromonas gingivalis:implications for the pathogenesis of adult periodontitis"Journal of Periodontal Research. 35(1). 1-9 (2000)

[Publications] N.Sugita: "Relevance of Fcγ RIIIa-158V-F polymorphism to recurrence of adult peiodontitis in Japanese patients"Clinical and Experimental Immunology. 117(2). 350-354 (1999)

[Publications] 村上伸也: "ヒアルロン酸の生物学的活性を考える"炎症(日本炎症学会雑誌). 19(6). 307-318 (1999)

[Publications] Nagasawa T.: "The role of salivary IgA antibody against periodontopathic bacteria"Oral Diseases. 5. 63-65 (1999)

[Publications] Ono,Nagasawa T.: "Periodontal status of selective IgA deficiency in a juvenile:case report"Journal of the International Academy of Periodontology. 1. 16-20 (1999)

[Publications] T.Nagasawa: "Oral administration of Porphromonas gingvalis fimbriae with cholera toxin induces antifibriae serum IgG,IgM,IgA and salivary IgA antibodies"Journal of Periodontal Research. 33. 169-174 (1999)

[Publications] Hirata M.: "Intrinsic inhibitor of inositol 1,4,5-trisphosphate binding"Mol.Cell.Biochem.. 190. 179-184 (1999)

[Publications] Yoshimura K.: "Use of phosphorofluoridate analogues of D-myo-inositol 1,4,5-trisphosphate to assess the involvement of ionic interactions in its recognition by the receptor and metabolising enzymes"Cell.Signal.. 11. 117-125 (1999)

[Publications] Caffrey,J.J.: "Multiple inositol polyphosphate phosphatase:Molecular cloning of the human form of an enzyme in endoplasmic reticulum that is upregulated during chondrocyte hypertrophy"FEBS Lett.. 442. 99-104 (1999)

[Publications] Takeuchi H.: "Membrane association of a new inositol 1,4,5-trisphosphate binding protein,p130 is not dependent on the pleckstrin homology domain"Chem.Phys.Lipids. 98. 35-47 (1999)

[Publications] Matsuki N.: "Antibodies against the PH domain of phospholipase C-1 inhibit Ins (1,4,5) P3-mediated Ca2^+ release from the endoplasmic reticulum"Biochem.Biophys.Res.Commun.. 260. 42-47 (1999)

[Publications] Yamamoto T.: "Involvement of EF hand motifs in the Ca2^+-dependent binding of the pleckstrin homology domain to phosphoinositides"Eur.J.Biochem.. 265. 481-490 (1999)

[Publications] Savoie CJ.: "The peptide binding motif of HLA-A^*0217"Immunogenetics. 49. 567-570 (1999)

[Publications] Matsuki N.: "Prevention of infection of influenza virus in DO6 mice,a human model,by a peptide vaccine prepared according to the cassette theory"Vaccine. 17. 1161-1168 (1999)

[Publications] Yoshitake S.: "HLA class II alleles in Japanese patients with inflammatory bowel disease"Tissue Antigens. 53. 350-358 (1999)

[Publications] Sano T.: "Spontaneous clustering of Thy-1 antigens on CD4^+ CD8^+ thymocytes lacking TCR engagement by MHC/peptode complexes"Eur.J.Immunol.. 29. 403-412 (1999)

[Publications] Nishimura H.: "MHC class II-dependent NK1.1^+ T cells are induced in mice by Salmonella infection"J.Immunol.. 162. 1573-1581 (1999)

[Publications] Nakano K.: "Prostaglandin E_2 inhibits alveolar bone resorption in experimental periodontitis in hamsters"Dentistry in Japan. 34. 108-111 (1998)

[Publications] Sawada K.: "Indentification of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis by subtractive hybridization"Infection and Immunity. 67. 5621-5625 (1999)

[Publications] 高柴正悟: "歯髄組織の創傷治癒時に特徴的に発現する遺伝子"歯科保存学雑誌. 42春季特別. 17-17 (1999)

[Publications] 大平泰資: "歯周組織創傷治癒過程に発現する遺伝子の同定"日本歯周病学会会誌. 41秋季特別. 117-117 (1999)

[Publications] Yamamoto T.: "Unique genes expressed in fibroblasts from human periodontal ligament in vitro"Program and Abstracts (11th International Conference on Periodontal Research). 18-18 (1999)

[Publications] Shogo Takashiba: "Concept for biological treatment of Periodontal disease"Program and Abstracts (3rd Asian Pacific Society of Periodontology). 20-20 (1999)

[Publications] 島田 顕: "組織培養による抜去歯の残存歯根膜からルートプレーニングした根面への細胞増殖とアルカリフォスファターゼ活性発現に関する研究"歯科保存学雑誌. 43(1). 254-261 (2000)

前田勝正九州大学, 歯学部, 教授 (00117243)

[Publications] 島田顕: "組織培養による抜去歯の残存歯根膜からルートプレーニングした根面への細胞増殖とアルカリフォスファターゼ活性発現に関する研究"歯科保存学雑誌. 43(1). 254-261 (2000)