2000 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Research Project

Project/Area Number	10307053
Research Institution	KYUSHU UNIVERSITY
Principal Investigator	前田勝正九州大学, 歯学研究院, 教授 (00117243)
Co-Investigator(Kenkyū-buntansha)	吉野宏広島大学, 歯学部, 助手 (50240338) 吉江弘正新潟大学, 歯学部, 教授 (20143787) 根本英二東北大学, 歯学研究科, 助手 (40292221) 平田雅人九州大学, 歯学研究院, 教授 (60136471) 石川烈東京医科歯科大学, 医歯学総合研究科, 教授 (10014151)
Keywords	インターロイキン1 / Fcレセプター / 分泌型IgA / Ip3結合タンパク質 / 歯周組織再生誘導法
Research Abstract	1.歯周病における生体防御反応の解析:細菌および細菌性因子の減弱に伴い炎症局所では、インターロイキン1βが減少しインターロイキン1αが主体となる反応を経過した後に骨形成に移行する事が明らかになった。早期発症型歯周炎においてFcレセプターの遺伝型の変異が観察された。又、好中球の産生する酵素により炎症性サイトカインの産生制御が行われていた。P.gingivalisに対する唾液中の特異的IgA抗体が、防御的に働く可能性が示唆された。炎症性細胞の機能発揮に関与するIP3結合タンパク質(p130)を発見しその作用機序更に、ヒトに於いて肝炎ワクチンに対する抗体産生応答が異なるTヘルパー細胞サブセットにより制御されている事を明らかにしている。 2.歯周病における組織修復の解析:種々のサイトカインにより線維芽細胞のヒアルロン酸産生が制御され組織修復に影響している事を明らかにした。歯槽骨の創傷治癒過程での遺伝子の解析で未知の7つの遺伝子が検出された。更に、歯周組織再生誘導法においてドキシサイクリンやEDTA併用が再生に有効な効果を示す動物実験結果やin vitroの実験結果を得た。以上、歯周病における生体防御反応及び組織修復について重要な所見を明らかにしており、新しい歯周治療法の開発を導く基礎的研究として十分な成果を挙げた。

Research Products
(26 results)

All Other

All Publications (26 results)

[Publications] Eiji Nemoto, et al.: "Cleavage of CD14 on human gingival fibroblasts cocultured with activated neutrophils is mediated by human leukocyte elastase resulting in down-regulation of lipopolysaccharide-induced IL-8 production"The Journal of Immunology. 165(10). 5807-5813 (2000)
[Publications] Shunji Sugawara, et al.: "Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingvalis leading to LPS-hyporesponsiveness"The Journal of Immunology. 165(1). 411-418 (2000)
[Publications] Tetsuo Kobayashi, et al.: "The Fcγ receptor genotype as a risk factor for generalized early-onset periodontitis in Japanese patients"Journal of Periodontology. 71(9). 1425-1432 (2000)
[Publications] 小島丈尚他: "初診時にActinobacillus actinomycetemcomitans, Porphyromonas gingivalisに対して高い血清IgG抗体価を示した歯周炎患者の長期的予後観察"日本歯周病学会誌. 41. 461-468 (2000)
[Publications] 長澤敏行他: "The Role of Salivary IgA Antibody against Periodontopathic Bacteria"Oral Disease. 5. 63-65 (1999)
[Publications] 長澤敏行他: "Oral administration of Porphyromonas gingivalis fimbriae with cholera toxin induces anti-fimbriae serum IgG,IgM,IgA and Salivary IgA antibody"Journal of Periodontal Research. 34. 169-174 (1999)
[Publications] 大野純一他: "Periodontal status of a Juvenile with Selective IgA Deficiency : A Case Report"Journal of International Academy of Periodontology. 1. 5-19 (1999)
[Publications] Kanematsu,T., et al.: "Domain organization of p130, PLC-related catalytically inactive protein, and structural basis for the lack of enzyme activity"Eur.J.Biochem.. 267. 2731-2737 (2000)
[Publications] Takeuchi,H., et al.: "Inhibition of calcium signalling by p130,PLC-related catalytically inactive protein : critical role of the p130PH domain"Biochem.J.. 349. 357-368 (2000)
[Publications] Tsujihata,Y., et al.: "Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response"J.Immunol.. 165. 3606-3611 (2000)
[Publications] Hermosura,M.C., et al.: "InsP4 facilitates store-operated calcium influx by inhibition of InsP3 5-phosphatase"Nature. 408. 735-740 (2000)
[Publications] Wataya,M., et al.: "Comparative analysis for HLA restriction and cytokine production of hepatitis B surface antigen-specific T cells between low and high antibody responders in vaccinated humans"J.Human Genet.. (in press). (2001)
[Publications] Hamaguchi,K., et al.: "Analysis of tumor necrosis factor-alpha promoter polymorphism in type 1 diabetes : HLA-B and -DRBI alleles are primarily associated with the disease in Japanese"Tissue Antigens. 55(1). 10-16 (2000)
[Publications] Inoue,T.K., et al.: "Linkage analysis of moyamoya disease on chromosome 6"J.Child Neurol.. 15(3). 179-182 (2000)
[Publications] Kawamura,K., et al.: "HLA-DR2-restricted responses to proteolipid protein 95-116 peptide cause autoimmune encephalitis in transgenic mice"J.Clin.Invest.. 105(7). 977-984 (2000)
[Publications] Ries S., et al.: "Opposing effects of Ras on p53 : transcriptional activation of mdm2 and induction of p19ARF"Cell. 103(2). 321-330 (2000)
[Publications] Shirasawa S., et al.: "Rnx deficiency results in congenital central hypoventilation"Nature Genet.. 24(3). 287-290 (2000)
[Publications] Toh H., et al.: "Changes at the floor of the peptide-binding groove induce a strong preference for proline at position 3 of the bound peptide : molecular dynamics simulations of HLA-A*0217"Biopolymers. 54(5). 318-327 (2000)
[Publications] Toh H., et al.: "Magnitude of structural changes of the T-cell receptor binding regions determine the strength of T-cell antagonism : molecular dynamics simulations of HLA-DR4 (DRBI*0405) complexed with analogue peptide"Protein Eng.. 13(6). 423-429 (2000)
[Publications] Baba I., et al.: "Involvement of deregulated epiregulin expression in tumorigenesis in vivo through activated Ki-Ras signaling pathway in human colon cancer cells"Cancer Res.. 60(24). 6886-6889 (2000)
[Publications] Fukui Y., et al.: "Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by its amino acid residue at a T cell receptor contact"Proc.Natl.Acad.Sci.USA. 97(25). 13760-13765 (2000)
[Publications] 大平泰資: "歯槽骨の創傷治癒に発現する遺伝子に関する研究"岡山歯学会誌. 19(1). 103-115 (2000)
[Publications] Taisuke Ohira, et al.: "Isolation of Differentially Expressed Genes in Periodontal Wound Healing"Journal of Dental Research 79 special issue. 79. 237 (2000)
[Publications] Taisuke Ohira, et al.: "Detection of unique genes expressed in periodontal wound healing"Journal of Dental Research 78 special issue. 78. 264 (1999)
[Publications] Chao-Yi Chang, et al.: "Evaluation of the Regenerative Effect of a 25% Doxycycline-Loaded Biodegradable Membrane for Guided Tissue Regeneration"J.Periodontol.. 71. 1086-1093 (2000)
[Publications] Yuji Kitamura, et al.: "Cleavage of Surface Proteins on Human T Cells by the Culture Supernatant of Porphyromonas gingivalis 381"Dentistry in Japan. 36. 109-112 (2000)

2000 Fiscal Year Annual Research Report

歯周病における生体防御と組織修復に関する総合的研究

Principal Investigator

前田 勝正 九州大学, 歯学研究院, 教授 (00117243)

Research Products

[Publications] Eiji Nemoto, et al.: "Cleavage of CD14 on human gingival fibroblasts cocultured with activated neutrophils is mediated by human leukocyte elastase resulting in down-regulation of lipopolysaccharide-induced IL-8 production"The Journal of Immunology. 165(10). 5807-5813 (2000)

[Publications] Shunji Sugawara, et al.: "Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingvalis leading to LPS-hyporesponsiveness"The Journal of Immunology. 165(1). 411-418 (2000)

[Publications] Tetsuo Kobayashi, et al.: "The Fcγ receptor genotype as a risk factor for generalized early-onset periodontitis in Japanese patients"Journal of Periodontology. 71(9). 1425-1432 (2000)

[Publications] 小島丈尚 他: "初診時にActinobacillus actinomycetemcomitans, Porphyromonas gingivalisに対して高い血清IgG抗体価を示した歯周炎患者の長期的予後観察"日本歯周病学会誌. 41. 461-468 (2000)

[Publications] 長澤敏行 他: "The Role of Salivary IgA Antibody against Periodontopathic Bacteria"Oral Disease. 5. 63-65 (1999)

[Publications] 長澤敏行 他: "Oral administration of Porphyromonas gingivalis fimbriae with cholera toxin induces anti-fimbriae serum IgG,IgM,IgA and Salivary IgA antibody"Journal of Periodontal Research. 34. 169-174 (1999)

[Publications] 大野純一 他: "Periodontal status of a Juvenile with Selective IgA Deficiency : A Case Report"Journal of International Academy of Periodontology. 1. 5-19 (1999)

[Publications] Kanematsu,T., et al.: "Domain organization of p130, PLC-related catalytically inactive protein, and structural basis for the lack of enzyme activity"Eur.J.Biochem.. 267. 2731-2737 (2000)

[Publications] Takeuchi,H., et al.: "Inhibition of calcium signalling by p130,PLC-related catalytically inactive protein : critical role of the p130PH domain"Biochem.J.. 349. 357-368 (2000)

[Publications] Tsujihata,Y., et al.: "Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response"J.Immunol.. 165. 3606-3611 (2000)

[Publications] Hermosura,M.C., et al.: "InsP4 facilitates store-operated calcium influx by inhibition of InsP3 5-phosphatase"Nature. 408. 735-740 (2000)

[Publications] Wataya,M., et al.: "Comparative analysis for HLA restriction and cytokine production of hepatitis B surface antigen-specific T cells between low and high antibody responders in vaccinated humans"J.Human Genet.. (in press). (2001)

[Publications] Hamaguchi,K., et al.: "Analysis of tumor necrosis factor-alpha promoter polymorphism in type 1 diabetes : HLA-B and -DRBI alleles are primarily associated with the disease in Japanese"Tissue Antigens. 55(1). 10-16 (2000)

[Publications] Inoue,T.K., et al.: "Linkage analysis of moyamoya disease on chromosome 6"J.Child Neurol.. 15(3). 179-182 (2000)

[Publications] Kawamura,K., et al.: "HLA-DR2-restricted responses to proteolipid protein 95-116 peptide cause autoimmune encephalitis in transgenic mice"J.Clin.Invest.. 105(7). 977-984 (2000)

[Publications] Ries S., et al.: "Opposing effects of Ras on p53 : transcriptional activation of mdm2 and induction of p19ARF"Cell. 103(2). 321-330 (2000)

[Publications] Shirasawa S., et al.: "Rnx deficiency results in congenital central hypoventilation"Nature Genet.. 24(3). 287-290 (2000)

[Publications] Toh H., et al.: "Changes at the floor of the peptide-binding groove induce a strong preference for proline at position 3 of the bound peptide : molecular dynamics simulations of HLA-A*0217"Biopolymers. 54(5). 318-327 (2000)

[Publications] Toh H., et al.: "Magnitude of structural changes of the T-cell receptor binding regions determine the strength of T-cell antagonism : molecular dynamics simulations of HLA-DR4 (DRBI*0405) complexed with analogue peptide"Protein Eng.. 13(6). 423-429 (2000)

[Publications] Baba I., et al.: "Involvement of deregulated epiregulin expression in tumorigenesis in vivo through activated Ki-Ras signaling pathway in human colon cancer cells"Cancer Res.. 60(24). 6886-6889 (2000)

[Publications] Fukui Y., et al.: "Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by its amino acid residue at a T cell receptor contact"Proc.Natl.Acad.Sci.USA. 97(25). 13760-13765 (2000)

[Publications] 大平泰資: "歯槽骨の創傷治癒に発現する遺伝子に関する研究"岡山歯学会誌. 19(1). 103-115 (2000)

[Publications] Taisuke Ohira, et al.: "Isolation of Differentially Expressed Genes in Periodontal Wound Healing"Journal of Dental Research 79 special issue. 79. 237 (2000)

[Publications] Taisuke Ohira, et al.: "Detection of unique genes expressed in periodontal wound healing"Journal of Dental Research 78 special issue. 78. 264 (1999)

[Publications] Chao-Yi Chang, et al.: "Evaluation of the Regenerative Effect of a 25% Doxycycline-Loaded Biodegradable Membrane for Guided Tissue Regeneration"J.Periodontol.. 71. 1086-1093 (2000)

[Publications] Yuji Kitamura, et al.: "Cleavage of Surface Proteins on Human T Cells by the Culture Supernatant of Porphyromonas gingivalis 381"Dentistry in Japan. 36. 109-112 (2000)

前田勝正九州大学, 歯学研究院, 教授 (00117243)

[Publications] 小島丈尚他: "初診時にActinobacillus actinomycetemcomitans, Porphyromonas gingivalisに対して高い血清IgG抗体価を示した歯周炎患者の長期的予後観察"日本歯周病学会誌. 41. 461-468 (2000)

[Publications] 長澤敏行他: "The Role of Salivary IgA Antibody against Periodontopathic Bacteria"Oral Disease. 5. 63-65 (1999)

[Publications] 長澤敏行他: "Oral administration of Porphyromonas gingivalis fimbriae with cholera toxin induces anti-fimbriae serum IgG,IgM,IgA and Salivary IgA antibody"Journal of Periodontal Research. 34. 169-174 (1999)

[Publications] 大野純一他: "Periodontal status of a Juvenile with Selective IgA Deficiency : A Case Report"Journal of International Academy of Periodontology. 1. 5-19 (1999)