2001 Fiscal Year Final Research Report Summary
TRANSCRPTIONAL REGgiATlON AS A NEW DRUG ACTION SITE-NOVEL TRANSCRPTIONAL FACTORS INVOLVED IN NEURAL DIFFERENTIATION AND MEMORY
| Project/Area Number |
10307057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
HIGUCHI Hiroshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, PROFESSOR, 大学院・医歯学総合研究科, 教授 (30150337)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Masayoshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSISTANT, 大学院・医歯学総合研究科, 助手 (40334677)
YAMAGUCHI Tsuyoshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCENCES. ASSISTANT, 大学院・医歯学総合研究科, 助手 (70323970)
MURAOKA Osamu GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, LECTURER, 大学院・医歯学総合研究科, 講師 (20283765)
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| Project Period (FY) |
1998 – 2001
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| Keywords | Neuropeptide Y / gene transcription / drug-induced regulation / Leptin / Zebrafish / transcriptional factor / NDF1 / JAK-STAT |
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| Research Abstract |
Neuropeptide Y (NPY) is a cotransmitter of sympathetic neurons and is expressed abundantly in the central nervous systm. By using the rat NPY gene, the drug-induced regulatory mechanism of transcription of the neuronspecific genes has been studied and the drug-regulated transcriptional factors have been explored. Previously we have identified the unique enhancer elements, such as nerve growth factor (NGF) response element (NGFRE) and calcium/calmodulirrdependent kinase response element (CaRE). When studying these elements, we identified novel transcriptional factors, such as NDF1 and Kheper.
These transcriptional factors are involved in synaptic plasticity and neural differentiation. Many neuroactive drugs seem to affect the function of these transcriptional factors. In 2001, we investigated especially the following contents.
1. Leptin-induced transactivation of NPY gene promoter
2. Cloning of zebrafish NDF homolog
3. Change in gene Expression of NPY subtype in brain
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