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2000 Fiscal Year Final Research Report Summary

CONTROL OF BIOLOGICAL PROCESSES BY STRONG MAGNETIC FIELDS

Research Project

Project/Area Number 10308037
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

SHIOKAWA Koichiro  UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF SCIENCE, PROFESSOR, 大学院・理学系研究科, 教授 (20037295)

Co-Investigator(Kenkyū-buntansha) IWASAKA Masakazu  UNIVERSITY OF TOKYO,GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院・医学系研究科, 講師 (90243922)
ANDO Joji  UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学系研究科, 教授 (20159528)
Project Period (FY) 1998 – 2000
KeywordsBiomagnetic effects / Xenopus laevis / Embryonic development / Magnetic orientation / Diamagnetic materials / dissolved oxygen / Magnetiphoresis / Magnetic stimulation
Research Abstract

In the present study, a possible influence of intense magnetic fields on cleavage patterns during the early embryonic development of frogs was studied.
We developed a microscope for real-time in situ observation of samples under superconducting magnet's bore. The eggs were exposed to horizontal magnetic fields, which were vertical to the animal-vegetable axis of eggs. Xenopus eggs with and without intense magnetic fields of up to 14 tesla were carefully observed during first to fifth cleavage for 3 hours. The normal control eggs showed vertical first and second cleavage planes and horizontal third planes, however, magnetic field exposed eggs tended to have declining third planes. By real-time observation under magnetic fields, almost 75% of eggs cleaved abnormally, and the number of abnormal declining cleavage lines increased depending on the magnetic flux density. Nevertheless, the percentage of abnormal tadpoles was the same between the control and the eggs cultured in magnetic fields. No teratogenic effects of an intense static magnetic field of 14 T were confirmed. The results indicated that early cleavages in Xenopus laevis were disordered by magnetic fields of 10 T order, independent on the magnetic field directions. The magnetic field effect did not remain until when they become tadpoles.
Also, we studied the properties of diamagnetic biological materials in static magnetic fields up to 14 T.We observed the phenomenon that the distribution of protein and cells became inhomogeneous around the center of the magnet. We also studied the possible effects of magnetic fields on the endothelial cell orientation. The cells oriented along the magnetically aligned collagen fibers. By utilizing near-infrared-oxygen monitor, oxy-/deoxy-hemoglobin concentration in a rat's head was measured under 8 T magnetic field, and discovered that the strong magnetic fields increased the population of oxyhemoglobin in rat tissue.

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Shiokawa,K.,Kai,M.,Higo,T.,Kaito,C.,Yokoska, et al.: "Maternal program of apoptosis activated shortly after midblastula transition by overexpression of S-adenosylmethionine decarboxylase in Xenopus early embryos"Comp.Biochem. Phys., Part B. 126. 149-155 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moroki,Y.,Takeuchi,S.,Igarashi,K.,Hara,H., et al.: "Effects of bromo-cyclic GMP and bromo-cyclic AMP on embryonic development of Xenopus laevis"Zool.Sci.. 17. 191-200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hara,H.,Uchida,S.,Yoshimura,H.,Aoki, et al.: "Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration"Biochim.Biophys.Acta,. 1492. 31-44 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oka,T.,Shiojima,I.,Monzen, Et al.: "Fibroblast growth factor plays a critical role in SM22alpha expression during Xenopus embryogenesis"Arterioscler Thromb Vasc.Biol.. April. 908-914 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mochizuki,T.,Karavanov,A.A.,Curtiss,P.E., et al.: "Xlim-1 and LIM domain binding protein 1 cooperate with various transcription factors in the regulation of the goosecoid promoter"Dev.Biol.. 224. 470-485 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kajita,E.,Wakiyama,M.,Miura,K.-I., et al.: "Isolation and characteization of Xenopus laevis aldolase B cDNA and expression patterns of aldolase A, B, and C genes in adult tissues, oocytes and embryos of Xenopus laevis"Biochim.Biophys.Acta. 1493. 101-118 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kai,M.,Higo,T.,Yokoska,J.,Kaito,: "Overexpression of S-adenosylmethionine decarboxylase (SAMDC) activates the maternal program of apoptosis shortly after MBT in Xenopus embryos"Int.J.Dev.Biol.. 44. 507-510 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moriwaki,J.,Kajita,E.,Kirikoshi: "Isolation of Xenopus frizzled-10A and frizzled-10B genomic clones and their expression in adult tissues and embryos"Biochem.Biophys.Res.Commun. 278. 377-384 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano,H.,Amemiya,S.,Shiokawa,K., et al.: "RNA interference for the organizer-specific gene Xlim-1 in Xenopus embryos"Biochem.Biophys.Res.Commun.. 274. 434-439 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kirikoshi,H.,Sagara,N.,Saitoh,T., et al.: "Molecular cloning and characterization of human FGF-20 on hcromosome 8p21.3-p22."Biochem.Biophys.Res.Commun. 274. 337-343 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoska,J.,Tsukamoto,T.,Miura,K.-I., et al.: "Cloning and characterization of mRNA capping enzyme and mRNA (guanine-7-)-methyltransferase cDNAs from Xenopus laevis"Biochem.Biophys.Res.Commun. 268. 617-624 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Liu,R.,Ueno,S.: "Calculating the activating function of nerve excitation in inhomogeneous volume conductor during magnetic stimulation using the finite element method"IEEE Transactions on Magnetics. 36:(4). 1796-1799 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsurita,G.,Nagawa,H.,Ueno,S., et al.: "Biological and morphological effects on the brain after exposure of rats to a 1439 MHz TDMA field"Bioelectromagnetics. 21. 364-371 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ichioka,S.,Minegishi,M.,Iwasaka,M., et al.: "High-intensity static magnetic fields modulate skin microcirculation and temperature in vivo"Bioelectromagnetics. 21. 183-188 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kotani,H.,Iwasaka,M.,Ueno,S., et al.: "Magnetic orientation of collagen and bone mixture"Journal of Applied Physic. 87. 6191-6193 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,K.,Korenaga,R.,Kamiya,A.,and Ando,J.: "Fluid shear stress activates Ca^<2+> influx into human endothelial cells via P2X4 purinoceptors"Circ.Res.. 87. 385-391 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagano,M.,and Shiokawa,K.: "Inhibition of transcription of class II, but not class III and I, genes in Xenopus postblastular embryos overexpressed with the TBP-binding protein, Dr 1 (NC2beta)"Biochem.Biophys.Res.Commun.. 265. 644-651 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamane,Y.,Tohno-oka,R.,Yamada,S.,Furuya,S.,Shiokawa,K., et al.: "Molecular characterization of Xenopus embryo heparan sulfate. Differential structural requirement for the specific binding to basic fibroblast growth factor and follistatin"J.Biol.Chem.. 273. 7375-7381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M.,Takeuchi,M.,Ueno,S.: "Polymerization and dissolution of fibrin under homogeneous magnetic fields"Journal of Applied Physics. 83:(11),. 6453-6455 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M.,Ueno,S.: "Bioluminescence under static magnetic fields"Journal of Applied Physics. 83:(11). 6456-6458 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M.,Ueno,S.: "Structure of water molecules under 14 T magnetic field"Journal of Applied Physics. 83:(11). 6459-6461 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M.,Ueno,S.: "Optical measurements of magnetophoresis of macromolecules"IEEE Transactions on Magnetics. 34:(4). 2129-2131 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M.,Yaoita,M.,Kono,T.,Ueno,S..: "Magnetically Disturbed Activity of Immobilized Catalase on an Electrode in Hydrogen-Peroxide Buffer"IEEE Transactions on Magnetics. July(in press.). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka,M,Miyakoshi,J.,Ueno,S.: "Optical Absorbance of Hemoglobin and Red Blood Cell Suspensions under Magnetic Fields"IEEE Transactions on Magnetics. July(in press.). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiokawa, K., Kai, M., Higo, T., Kaito, C., Yokoska, et al.: "Maternal program of apoptosis activated shortly after midblastula transition by overexpression of S-adenosylmethionine decarboxylase in Xenopus early embryos"Comp.Biochem.Phys.. Part B126. 149-155 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moroki, Y., Takeuchi, S., Igarashi, K., Hara, H., et al.: "Effects of bromo-cyclic GMP and bromo-cyclic AMP on embryonic development of Xenopus laevis"Zool.Sci.. 17. 191-200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hara, H., Uchida, S., Yoshimura, H., Aoki, et al.: "Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration"Biochim.Biophys.Acta. 1492. 31-44 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oka, T., Shiojima, I., Monzen, et al.: "Fibroblast growth factor plays a critical role in SM22alpha expression during Xenopus embryogenesis"Arterioscler Thromb Vasc.Biol.. 908-914 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mochizuki, T., Karavanov, A.A., Curtiss, P.E., et al.: "Xlim-1 and LIM domain binding protein 1 cooperate with various transcription factors in the regulation of the goosecoid promoter"Dev.Biol.. 224. 470-485 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kajita, E., Wakiyama, M., Miura, K.-I., et al.: "Isolation and characteization of Xenopus laevis aldolase B cDNA and expression patterns of aldolase A, B, and C genes in adult tissues, oocytes and embryos of Xenopus laevis"Biochim.Biophys.Acta. 1493. 101-118 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsurita, G., Nagawa, H., Ueno, S., et al.: "Biological and morphological effects on the brain after exposure of rats to a 1439 MHz TDMA field"Bioelectromagnetics. 21. 364-371 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichioka, S., Minegishi, M., Iwasaka, M., et al.: "High-intensity static magnetic fields modulate skin microcirculation and temperature in vivo"Bioelectromagnetics. 21. 183-188 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kotani, H., Iwasaka, M., Ueno, S., et al.: "Magnetic orientation of collagen and bone mixture"Journal of Applied Physic. 87. 6191-6193 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, K., Korenaga, R., Kamiya, A., and Ando, J.: "Fluid shear stress activates Ca2+ influx into human endothelial cells via P2X4 purinoceptors"Circ.Res.. 87. 385-391 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagano, M., and Shiokawa, K.: "Inhibition of transcription of class II, but not class III and I, genes in Xenopus postblastular embryos overexpressed with the TBP-binding protein, Dr 1 (NC2beta)"Biochem.Biophys.Res.Commun.. 265. 644-651 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamane, Y., Tohno-oka, R., Yamada, S., Furuya, S., Shiokawa, K., et al.: "Molecular characterization of Xenopus embryo heparan sulfate. Differential structural requirement for the specific binding to basic fibroblast growth factor and follistatin"J.Biol.Chem.. 273. 7375-7381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kai, M., Higo, T., Yokoska, J., Kaito, et al.: "Overexpression of S-adenosylmethionine decarboxylase (SAMDC) activates the maternal program of apoptosis shortly after MBT in Xenopus embryos"Int.J.Dev.Biol.. 44. 507-510 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriwaki, J., Kajita, E., Kirikoshi, et al.: "Isolation of Xenopus frizzled-10A and frizzled-10B genomic clones and their expression in adult tissues and embryos"Biochem.Biophys.Res.Commun. 278. 377-384 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakano, H., Amemiya, S., Shiokawa, K., et al.: "RNA interference for the organizer-specific gene Xlim-1 in Xenopus embryos"Biochem.Biophys.Res.Commun.. 274. 434-439 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kirikoshi, H., Sagara, N., Saitoh, T., et al.: "Molecular cloning and characterization of human FGF-20 on hcromosome 8p21.3-p22."Biochem.Biophys.Res.Commun. 274. 337-343 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoska, J., Tsukamoto, T., Miura, K.-I., et al.: "Cloning and characterization of mRNA capping enzyme and mRNA (guanine-7-)-methvltransferase cDNAs from Xenopus laevis"Biochem.Biophys.Res.Commun. 268. 617-624 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Liu, R., Ueno, S.: "Calculating the activating function of nerve excitation in inhomogeneous volume conductor during magnetic stimulation using the finite element method"IEEE Transactions on Magnetics. 36 : (4). 1796-1799 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M., Takeuchi, M., Ueno, S.: "Polymerization and dissolution of fibrin under homogeneous magnetic fields"Journal of Applied Physics. 83 : (11). 6453-6455 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M., Ueno, S.: "Bioluminescence under static magnetic fields"Journal of Applied Physics. 83 : (11). 6456-6458 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M., Ueno, S.: "Structure of water molecules under 14 T magnetic field"Journal of Applied Physics. 83 : (11). 6459-6461 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M., Ueno, S.: "Optical measurements of magnetophoresis of macromolecules"IEEE Transactions on Magnetics. 34 : (4). 2129-2131 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M., Yaoita, M., Kono, T., Ueno, S.: "Magnetically Disturbed Activity of Immobilized Catalase on an Electrode in Hydrogen-Peroxide Buffer"IEEE Transactions on Magnetics, 2001. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka, M, Miyakoshi, J., Ueno, S.: "Optical Absorbance of Hemoglobin and Red Blood Cell Suspensions under Magnetic Fields"IEEE Transactions on Magnetics, 2001. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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