1999 Fiscal Year Final Research Report Summary
Molecular design of artificial glycoconjugate polymers and their applications to materials biotechnology
| Project/Area Number |
10450352
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子合成
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Kazukiyo Nagoya University, Graduate school of Engineering, Professor, 工学研究科, 教授 (10023483)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Kazunori Nagoya University, Graduate school of Engineering, Associate Professor, 工学研究科, 助手 (60283389)
NISHIDA Yoshihiro Nagoya University, Graduate school of Engineering, Associate Professor, 工学研究科, 助教授 (80183896)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Glycopolymer / Glycotechnology / Recognition / Materials Life Science |
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| Research Abstract |
1. Molecular design of Artificial Glycoconjugate Polymers
Several convenient synthetic routes of biologically important, complex oligosaccharides have been developed and then these oligosaccharides have been converted to the corresponding glycopolymers.
(1) Gb3-trisaccharide (Galα1-4-Galβ1-4-Glcb) was synthesized from lactose and converted to acrylamide-type monomeric substance, followed by polymerization.
(2) Mannose trisaccharide was synthesized by a convenient route and introduced to glycopolymer.
2. Characterization of the glycopolymers.
(1) Cluster effect in glyco recognition was investigated by the interactions with lectins.
(2) Carbohydrate-carbohydrate interaction was investigated by p-A isotherms abd surface plasmon resonance techniques.
3. Applications to Materials Biotechnology
(1) Trapping of influenza viruses by sialyl-lactose-carrying glycopolymer
(2) Neutralization of E-coli O-157 toxins by Gb3-trisaccharide-carrying glycopolymer
(3) Production of antibodies by sulfated-galactose-carrying glycopolymers
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