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2001 Fiscal Year Final Research Report Summary

Identification and cloning of JH receptor

Research Project

Project/Area Number 10460020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionMie University

Principal Investigator

CHINZEI Yasuo  Mie Univ., Med., Professor, 医学部, 教授 (60024709)

Co-Investigator(Kenkyū-buntansha) MIURA Ken  Mie Univ., Med., Lecturer, 医学部, 講師 (60219582)
Project Period (FY) 1998 – 2001
KeywordsJuvenile hormone / metamorphosis / transcription regulation / cDNA library / cyanoprotein
Research Abstract

Juvenile hormone (JH) regulates a wide range of insect physiological phenomena such as metamorphosis, diapause and polymorphysm, which are very important for insect development and complete of life cycle. However JH mode of action is still unknown, particularly JH receptor and signal transduction in the target cells are not elucidated yet.
In this study we aimed to make clear a moleculara level of JH action including JH receptor and the regulation of gene expression through the cloning of JH responsive gene and their responsive transcription factor, and identification and cloning of JH receptor.
We studied the cloning of JH responsive transcription factor (JHRTF), by the experiments combined that showed below, using JH responsive element (JHRE) of cyanoprotein (CP) gene and JH treated fat body cDNA library, in the bean bug Riptortus clavatus.
(1) JHRTF is screened by south-Western method and phage-display method from cDNA library transformed In the expression vector or Phase by using labelled JHRE as a probe.
(2) JHRTF Is screened by one-hybrid method, Introducing DNA constract which constructed with JHR E of CP, and cDNA-Gal4 fusion library Into yeast cells.
(3) JHRTF Is screened by two-hybrid method, using cDNA-jGla4 fusion library and Gla4 fusion DNA construct with of known transcription factor.
We have tried to study the above experiments, It was unsuccessful, and we could not get the JHRDT clone as a result so far.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Tetsuro Shinoda, et al.: "Cloning and functional expression of achitinase cDNA from the common cutworm, Spodoptera litura, using a recombinant"Insect Biochem. Mol. Biol.. 31. 521-532 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuhiko Ando, et al.: "Five cases of Diphyllobothrium nihonkaiense infection with discovery of plerocercoides from an infective source"J. Parasitol.. 87. 96-100 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Furuta, et al.: "Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombinant"J. Gen. Virol.. 82. 307-312 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Yuda, et al.: "von Willebrand Factor A domain-related protein : a novel microneme protein of the malaria ookinete highly conserved"Mol. Biochem. Partasitol.. 116. 65-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toru Kariu, et al.: "Essential function of MAEBL in malarial sporozoite infection of the mosquito salivary gland"J. Exp. Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hubert Woitasek et al.: "Analysis of the involvement of the 3'-untraslated regions in regulating mRNA stability for vitellogenin, cyanoprotein a and"Insect Biochem. Mol. Biol.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuro Shinoda, et.al.: "Cloning and functional expression of achitinase cDNA from the common cutworm, Spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene"Insect Biochem. Mol. Biol.. 31. 521-532 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko Ando, et.al.: "Five cases of Dinhyllobothrium nihonkaiense infection with discovery of plerocercoides from an infective source, Oncorhynchus mason ishkawae"J. Parasitol.. 87. 96-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Furuta, et.al.: "Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombmant baculoviruses to silkworm larvae is frastically enhanced when administered with Anomala cuprea entomopoxvirus spindles"J. Gen. Virol.. 82. 307-312 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Yuda, et.al.: "von Willebrand Factor A domain-related protein : a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites"Mol. Biochem. Paratasitol.. 116. 65-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toru Kariu,et.al.: "Essential function of MAEBL in malarial sporozoite infection of the mosquito salivary gland"J. Exp. Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hubert Woitasek et.al.: "Analysis of the involvement of the 3'-untranslated regions in regulating mRNA stability for vitellogenin, cyanoprotein a and cyanoprotein b from the bean bug, Riptortus clavatus"Insect Biochem. Mol. Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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