2000 Fiscal Year Final Research Report Summary
Construction of an EST database for Bombyx mori
| Project/Area Number |
10460027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
MITA Kazuei National Institute of Radiological Sciences, Genome Research Group, Researcher, 第2研究グループ, 研究員 (30159165)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIMURA Sachiko National Institute of Radiological Sciences, Researcher, 生物影響研究部, 研究員 (40158754)
NENOI Mitsuru National Institute of Radiological Sciences, Researcher, 生物影響研究部, 研究員 (10164659)
SHIMADA Toru University of Tokyo, Department of Agricultural and Environmental Biology, Associate Professor, 農学生命科学研究科, 助教授 (20202111)
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| Project Period (FY) |
1998 – 2000
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| Keywords | silkworm, Bombyx mori / multi-cellular organism / cDNA collection / EST database / cDNA library / gene classification / gene expression profile |
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| Research Abstract |
To build a foundation for the complete genome analysis of Bombyx mori, we have constructed an EST database. Since gene expression patterns deeply depend on tissues as well as developmental stages, we analyzed many cDNA libraries prepared from various tissues and different developmental stages to cover the whole set of Bombyx genes. So far, the Bombyx EST db contains 35,000 ESTs from 36 cDNA libraries, which are grouped into about 11,000 non-redundant ESTs with the average length of 1.25 kb. The comparison with FlyBase suggests that the present EST database, "SilkBase," covers more than 55% of all genes of Bombyx. The fraction of library-specific ESTs in each cDNA library indicates that we have not yet reached saturation, showing the validity of our strategy for constructing an EST database to cover all genes. To tackle the coming saturation problem, we have checked two methods, subtraction and normalization, to increase coverage and decrease the number of housekeeping genes, resulting in 5-11% increase of library-specific ESTs. The identification of a number of novel genes and comprehensive cloning of gene families have already emerged from SilkBase search. Direct links of SilkBase with FlyBase and WormBase provide ready identification of candidate Lepidoptera-specific genes.
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[Journal Article] The construction of EST database of Bombyx mori and its application.2002
Author(s)
Mita, K., Morimyo, M., Okano, K., Koike, Y., Nohata, J., Suzuki, M.G., Shimada, T.
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Journal Title
Current Sci. 83
Pages: 426-431
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A homologue of the Drosophila doublesex gene is transcribed into sex-specific mRNA isoforms in the silkworm, Bombyx mori.2001
Author(s)
Ohbayashi, F., Suzuki, M.G., Mita, K., Okano, K., Shimada, T.
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Journal Title
Comp.Biochem.Physiol.Ser.B 128
Pages: 145-158
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and particular expression of ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori.2001
Author(s)
Quan, G-X., Mita, K., Okano, K., Shimada, T., Ugajin, N., Goto, N., Kannke, E., Kawasaki, H.
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Journal Title
Insect Mol.Biol. 31(1)
Pages: 97-103
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of acyl-CoA-binding protein (ACBP) in the pheromone gland of the silkworm, Bombyx mori.2001
Author(s)
Matsumoto, S., Yoshiga, T., Yokoyama, N., Iwanaga, M., Koshiba, S., Kigawa, T., Hirota, H., Yokoyama, S., Okano, K., Mita, K., Shimada, T., Tatsuki, S.
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Journal Title
Insect Biochem Mol Biol. 31
Pages: 603-609
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of the 90-kDa heat shock protein coding genes in the Lepidoptera Bombyx mori and Spodoptera frugiperda2001
Author(s)
Landais, I., Pommet, J-M., Mita, K., Nohata, J., Gimenez, S., Fournier, P., Devauchella, G., Duonor-Cerutti, M., Ogliastro, M.H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A bacteria-induced, intracellular serpin in granular hemocytes of Manduca sexta.2001
Author(s)
Gan, H., Wang, Y., Jiang, H., Mita, K., Kanost, M.R.
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Journal Title
Insect Biochem Mol.Biol. 31
Pages: 887-898
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mass isolation of cuticle protein cDNAs from wing discs of Bombyx mori and their characterizations.2001
Author(s)
Takeda, M., Mita, K., Quan, G., Shimada, T., Okano, K., Kanke, E., Kawasaki, H.
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Journal Title
Insect Biochem Mol.Biol. 31(10)
Pages: 1019-1028
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation and expression of an ecdysteroid-inducible neutral endopeptidase 24.11-like gene in wing discs of Bombyx mori.2001
Author(s)
Zhao, K., Mita, K., Shimada, T., Okano, K., Quan, G., Kanke, E., Kawasaki, H.
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Journal Title
Insect Biochem Mol.Biol. 31(12)
Pages: 1213-1219
Description
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[Journal Article] Characterization of JDP genes, an evolutionarily conserved J domain-only protein family, from human and moths.2000
Author(s)
Lee, A.J., Hahn, Y., Yun, J.H., Mita, K., Chung, J.H.
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Journal Title
Biochim.Biophys.Acta 1491
Pages: 355-363
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] cDNA cloning of acyl-CoA desaturase homologs in the silkworm, Bombyx mori.2000
Author(s)
Yoshiga, T., Okano, K., Mita., K., Shimada, T., Matsumoto, S.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect, and plant evolution.2000
Author(s)
Hahn, Y., Lee, Y.J., Yun, J.H., Yang, S.K., Park, C.W., Mita, K., Huh, T-L., Rhee, M., Chung, J.H.
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Journal Title
FEBS Lett. 482
Pages: 31-36
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A covariant change of the two highly conserved bases within the GTPase-associated center of ribosomal 28S rRNA in silkworms and other moths.2000
Author(s)
Uchiumi, T., Nomura, T., Shimizu, T., Katakai, Y., Mita, K., Koike, Y., Nakagaki, M., Taira, H., Hachimori, A.
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Journal Title
J.Biol.Chem. 273
Pages: 35116-35121
Description
「研究成果報告書概要(欧文)」より
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