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2001 Fiscal Year Final Research Report Summary

Genome rearrangements of indigenous soybean bradyrhizobia and their ecological significance

Research Project

Project/Area Number 10460028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

MINAMISAWA Kiwamu  Graduate School of Life Sciences, Tohoku University, Professor, 大学院・生命科学研究科, 教授 (70167667)

Co-Investigator(Kenkyū-buntansha) TSUDA Masataka  Graduate School of Life Sciences, Tohoku University, Professor, 大学院・生命科学研究科, 教授 (90172022)
MITSUI Hisayuki  Graduate School of Life Sciences, Tohoku University, Associate Professor, 大学院・生命科学研究科, 助教授 (40261466)
Project Period (FY) 1998 – 2001
KeywordsBradyrhizobium japonicum / genetic ecology / insertion sequence / nodulation / soil bacteria / soybean / stress resistance
Research Abstract

In order to understand the generation and ecological roles of Bradyrhizobium japonicum HRS strains possessing high copy numbers of insertion sequences (IS), we have investigated (1) geographical and phylogenetic distributions of HRS strains, (2) their excellent features in field ecosystem, (3) their genome rearrangements, and (4) horizontal transfer of nodulation (nod) gene from HRS strains. We found a correlation among the IS copy number, phylogenetic position, serogroup and growth rate of HRS strains, which are derived from USA, China and Japan. Nodule occupancy of HRS strains increased in a water-logged soil condition. Moreover, HRS strains showed stress resistance. The nif gene cluster of HRS strain were subjected to DNA rearrangement probably by IS elements. We have observed horizontal transfer of nod genes from B. japonicum HRS strain to B. elkanii USDA94 mutant lacking common nod genes as a recipient. In this project, we could show the ecological and genetic features of B. japonicum HRS strains.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Minamisawa, K.: "Horizontal transfer of nodulation genes in soil and microcosm from Bradyrhizobium japonicum to B. elkanii"Microbe Environ.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamisawa, K.: "Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ"FEMS Microbiol. Ecol.. 29. 171-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isawa, T.: "IS1631 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains with high copy numbers of repeated sequences RSα and RSβ"Appl. Environ. Microbiol.. 65. 3493-3501 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuda, M.: "Mobile catabolic gene in bacteria"J. Bio. Bioeng.. 87. 401-410 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamisawa, K.: "New Bradyrhizobium japonicum that possess high copy numbers of the repeated sequence RSα"Appl. Environ. Microbiol.. 64. 1845-1851 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito, A.: "Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium japonicum"FEMS Microbiol. Eco.. 25. 277-286 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamisawa, K.: "Chapter 8: Genetic ecology of soybean bradyrhizobia. In Soil Biochemistry Volume 10"Marcel Dekker, New York. 519(348-377) (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamisawa, K et al.: "Horizontal transfer of nodulation genes in soil and microcosm from Bradyrhizobium japonicum to B. elkanii"Microbe Environ.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamisawa, K. et al.: "Genetic ecology of soybean bradyrhizobia"Marecel Dekker, Soil Biochemistry Vol. 10.,(2000), Edited by J. Bollag and G. Stotzky. pp. 348-377, New York.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamisawa, K. et al.: "Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ"FEMS Microbiol. Ecol.. 29. 171-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isawa, T. et al.: "IS1637 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains w|th high copy numbers of repeated sequences RSα and RSβ"Appl. Environ. Microbiol.. 65. 3493-3501 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M. et al.: "Mobile catabolic gene in bacteria"J. Bio. Bioeng. 87. 401-410 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamisawa, K. et al.: "New Bradyrhizobium japonicum that possess high copy numbers of the repeated sequence RSα"Appl. Environ. Microbiol.. 65. 1845-1851 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, A. et al.: "Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium"FEMS Microbiol. Eco.. 25. 277-286 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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