1999 Fiscal Year Final Research Report Summary
Transcription Factors Involved in the induction of Jasmonate-Primary Responsive Gene in Plant Cells
| Project/Area Number |
10460038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
NAKAMURA Kenzo Nagoya University, Graduate School of Bioagricultural Science, Professor, 大学院・生命農学研究科, 教授 (80164292)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Jasmonic acid / Transformed plant cells / Promoter / cis-Elements / Ornithine decarboxylase / ODC / Trans-factors |
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| Research Abstract |
Transcription of the gene for ornithine decarboxylase (ODC) of tobacco BY-2 cells is activated even in the presence of an inhibitor of protein synthesis. A nuclear gene for ODC isolated from tobacco did not contain intron, and the fusion gene composed of the ODC promoter and the GUS reporter gene (ODC : GUS) showed early response to MeJA in transformed tobacco BY-2 cells. Although expression of the ODC: GUS fusion gene was not affected by salicylic acid (SA) alone, MeJA-inducible expression of the fusion gene was enhanced to about 2-fold by the presence of low concentrations of SA or aspirin. Thus, unlike MeJA-inducible gene impression in wounded leaves, JA-inducible expression of the ODC gene in tobacco BY-2 cells is not inhibited by SA. Analysis using serial deletions and internal deletions of the ODC gene promoter revealed that the sequence between -1457 and -1351 and the sequence between -943 and -746 are both important in the MeJA-inducible expression of the ODC promoter. These sequences did not contain several sequence motifs such as G-box that had been implicated to be involved in the MeJA-inducible expression of secondary-responsive genes in plants. Nuclear extracts prepared from tobacco BY-2 cells contained activities which specifically bind to the two regions of the ODC promoter important for the MeJA-responsible expression. Treatment of tobacco cells with MeJA did not affect the binding activities.
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[Publications] Imanishi, S., Hashizume, K., Kojima, H., Ichihara, A. and Nakamura, K.: "An mRNA of tobacco cell, which is rapidly incible by methyl jasmonate in the presence of cycloheximide, codes for a putative glycosyltransferase"Plant Cell Physiol.. 39. 202-211 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Imanishi, S., Hashizume, K., Nakakita, M., Kojima, H., Matsubayashi, Y., Hashimoto, T., Sakagami, Y., Yamada, Y. and Nakamura, K.: "Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosysthesis in tobacco cell cultures"Plant Mol. Biol.. 38. 1101-1111 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Imanishi, S., Nakakita, M., Yamashita, K., Furuta, A., Utsuno, K., Muramoto, N., Kojima, H. and Nakamura, K.: "Aspirin and salicylic acid do not inhibit methyl jasmonate-inducible expression of a gene for ornithine decarboxylase in tobacco BY-2 cells"Biosci. Biotechnol. Biochem.. 64. 125-133 (2000)
Description
「研究成果報告書概要(欧文)」より
