2000 Fiscal Year Final Research Report Summary
Effect of antibiotics on sedimentary microbiota as characterized by biomarker analysis.
| Project/Area Number |
10460088
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
MATSUDA Osamu Hiroshima University, Faculty of Applied Biological Science, Professor, 生物生産学部, 教授 (60034469)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Toshiya Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (00253120)
YAMAMOTO Tamiji Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (40240105)
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| Project Period (FY) |
1998 – 2000
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| Keywords | antibiotics / microbiota / biomarker analysis / aquaculture / phospholipid / streptomycin / PLFA / sediment |
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| Research Abstract |
Antibiotics are used in both therapeutic and prophylactic treatment of the stocks. Because of wasted feed and low digestive efficiencies of some antibiotics, a substantial fraction of the drug can be released to the environment and is deposited to the sediment. Antibiotics are also used to treat the larvae in hatcheries. After treating the shellfish larvae in seawater containing streptomycin, the seawater has been discharged into the adjacent coastal environment. Thus antibiotic use and its accumulation in the sediment may influence the natural sedimentary microbiota. In order to know the impact of antibiotics on sedimentary microbial biomass, and its community structure, phospholipid ester-linked fatty acid(PLFA)analysis of sediment supplemented with different concentrations of streptomycin was carried out.
Sediment parameters did not show significant changes during the incubation. Concentrations of streptomycin in seawater were higher than that in sediments. Furthermore, concentrations of streptomycin increased during the incubation period due to the addition of streptomycin. Sedimentary microbial biomass, as calculated by total PLFA, was decreased in all the three concentrations of streptomycin added sediments. The decrease of microbial biomass over control was in the order of 20 ppm > 10ppm > 5ppm. At the end of the incubation, levels of total PLFA in sediments(10 and 20 ppm streptomycin)were either high or comparable to the control values indicating that sedimentary microbial populations might have developed antibiotic resistance. The pattern of PLFA groups in sediments was different among the three concentrations of streptomycin used. Cluster analysis of PLFA profiles revealed the changes in microbial communities of sediments amended with streptomycin. The results of multidimensional scaling plot also indicated changes in PLFA profiles due to the addition of streptomycin and also disruption of the natural microbiota.
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Research Products
(5 results)
