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2000 Fiscal Year Final Research Report Summary

Analysis of the regulatory mechanism of the differentiation of epidermal keratinocytes mediated by fat-soluble ligands

Research Project

Project/Area Number 10460117
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionTOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY

Principal Investigator

UEHARA Kohkichi  Tokyo Univ. Agricul. & Technol. Dept. of Tissue Physiol. Prof., 農学部, 教授 (10014953)

Co-Investigator(Kenkyū-buntansha) ARAI Koji  Tokyo Univ. Agricul. & Technol. Dept. of Tissue Physiol. Assis. Prof., 農学部, 助手 (70293016)
ARAI Katsuhiko  Tokyo Univ. Agricul. & Technol. Dept. of Vet Clinic. Assoc. Prof., 農学部, 助教授 (60175940)
MATSUDA Hiroshi  Tokyo Univ. Agricul. & Technol. Dept. of Tissue Physiol. Prof., 農学部, 教授 (80145820)
Project Period (FY) 1998 – 2000
KeywordsKERATINOCYTES / DIFFERENTIATION / KERATOHYALIN / MONOCLONAL ANTIBODY / 分化
Research Abstract

The monoclonal antibody reacted with keratohyalin granules was cloned. This antibody, MAbKH1 was generated by the immunization of cytoskeletal fraction prepared from canine mammary cacinoma against mice. MAbKH1 reacted the epidermis of several animal species including dog, rat, cattle and pig. In neonatal rat tissue, MAbKH1 also reacted with arterial and intestinal smooth muscle in addition of keratohyalin granules and in adult rat, this positive reaction was restricted in the epidermis immunohistochemically. Immunoblot analysis revealed that MAbKH1-recognizing protein in addition of 165 kDa and 200 kDa bands in both neonatal and adult rat skin extracts. The 1.7 kbp cDNA was cloned by the immunoscreening with MAbKH1 against lamda gt 11 constructed neonatal rat brain cDNA library. In vitro, expression of KH1 protein was upregulated under high calcium conditions accompanied with increased expression of transglutaminase, which is a molecular marker for the terminal differentiation of keratinocytes in rat keratinocytes. By double-labeled immunofluorescent staining with MAbKH1 and anti-cytokeratin rabbit serum, keratohyalin granules appeared by higher calcium ion were strongly stained with MAbKH1 in the cytoplasm of keratinocytes, suggesting that 55 kDa KH1 protein is one of constitution of keratohyalin granules. In conclusion, we characterized a new keratohyalin protein, which gave a molecular weight of 55 kDa and demonstrated that expression of this protein was regulated by the concentration of calcium ion in vitro.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] K.ARAI, et al: "RT-PCR coupled with HPLC analysis of COL1 and COL3 mRNA enpressed in horse tendon"J.Vet.Med.Sci. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.ARAI et al: "Characterization of isotype-specific regions of five classes of canine beta-tubulin and their expression in several tissues and cell culture"J.Vet.Med.Sci.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.NIHEI, et al: "Argnine as an exacerbating factor for gluromerulonephritis in rats fed a methionine-threonine-supplemented low casein diet"Biosci.Biotechnol. Biochem. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.TANAKA, et al: "Matrix metalloproteinase-9 production, a newly identified function of mast cell progenitors, is downregulated by c-kit receptor activation"Blood. 94. 2390-2395 (1999)

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      「研究成果報告書概要(和文)」より
  • [Publications] OHSHIMA, et al: "Secretion of inhibin A, inhibin B and inhibin pro-alpha C.during the oestrus cycle of the golden hamster"J.Endocrinol.. 162. 451-456 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.ARAI, et al.: "Inhibition of ovarian follicular development associated with a decrease inlutenizing hormone level during the oestrus cycle of the rat"Endocrine.J.. 45. 539-546 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.ARAI, A.EBIHARA, Y.KASASHIMA, A.KUWANO and T.YOSIHARA: "RT-PCR coupled with HPLC analysis of COL1 and COL3 mRNA expressed in horse tendon."J.Vet Med Sci. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.ARAI, K.MARUO, K.Y.ARAI, K.UEHARA and H,MATSUDA: "Characterization of isotype-specific regions of five classes of canine beta-tubulin and their expression in several tissues and cell culture."J.Vet Med Sci. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.NIHEI, K.ARAI, Y.MIURA and K.YAGASAKI: "Arginine as an exacerbating factor for glomerulonephritis in rats fed a methionine-threonine-supplemented low casein diet."Biosci.Biotechnol.Biochem.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.TANAKA, K.ARAI, Y.KITAMURA and H.MATSUDA: "Matrix metalloproteinase-9 production, a newly identified function of mast cell progenitors, is downregulated by c-kit receptor activation."Blood. 94. 2390-2395 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.OHSHIMA, H.KISHI, M.ITOH, G.WATANABE, K.ARAI, K.UEHARA, N.P.GROOME, K.TAYA: "Secretion of inhibin A, inhibin B and inhibin pro-alphaC during the oestrous cycle of thegolden hamster (Mesocricetus auratus)."J.Endocrinol. 162. 451-456 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.ARAI, K.OHSHIMA, G.WATANABE, K.ARAI, K.UEHARA and K.TAYA: "Inhibition of ovarian follicular development associated with a decrease in luteinizing hormone levels during the estrous cycle of the rat."Endocrine J.. 45. 539-546 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.AKAI, A.KIMUTA, K.ARAI, K.UEHARA and R.HATA: "Fine structural analysis of the unique 5'-region of human COL1A2 gene containing two regions of dinucleotide repeats adjacent to the transcriptional start site."Connective Tissue. 30. 1-6 (1998)

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      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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