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2000 Fiscal Year Final Research Report Summary

Interferon-tau and implantation : Prevention of early pregnancy losses.

Research Project

Project/Area Number 10460127
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

IMAKAWA Kazuhiko  Graduate School of Agriculture & Agricultural Life Science, THE UNIVERSITY OF TOKYO Associate Professor, 大学院・農学生命科学研究科, 助教授 (00291956)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Hiroyuki  Graduate School of Agriculture & Agricultural Life Science, THE UNIVERSITY OF TOKYO Associate Professor, 大学院・農学生命科学研究科, 助教授 (40155891)
SAWASAKI Toru  Graduate School of Agriculture & Agricultural Life Science, THE UNIVERSITY OF TOKYO Professor, 大学院・農学生命科学研究科, 教授 (00012047)
SAKAI Senkichi  Graduate School of Agriculture & Agricultural Life Science, THE UNIVERSITY OF TOKYO Professor, 大学院・農学生命科学研究科, 教授 (80114487)
AOKI Fugaku  Graduate School of Frontier Sciences, THE UNIVERSITY OF TOKYO Associate Professor, 大学院・新領域創成科学研究科, 助教授 (20175160)
Project Period (FY) 1998 – 2000
Keywordsmeat (domestic) animals / implantation / interferon-tau / enhancer / promoter / silencer / trophoblast / IP10
Research Abstract

Implantation is a complex process involving maternal-fetal communication, their attachment, trophoblast invasion to maternal endometrium, and formation and functioning of the placenta. Interferon-tau (IFNτ), produced by the trophectoderm of ruminant ungulates, is known as a conceptus factor, which elicits maternal recognition of pregnancy, resulting in successful progression of implant6ation and the establishment of pregnancy. However, molecular mechanisms by which IFNτ is regulated have not been determined. In the present investigation, therefore, the objectives are to elucidate mechanisms of IFNτ gene transcription, to control the process of implantation through the manipulation of IFNτ expression, and to improve reproductive efficiency of domestic animals. For the transcription of IFNτ gene, promoter, enhancer and silencer region from the upstream region were determined through deletion, mutation and gel-shift assays. Those experiments were done by using human choriocarcinoma JEG3 cells, but presently, trophoblast cell line established from Shiba goat placenta is being used. Numerous subtraction studies were also executed : those involve days 14-15 and 19-20 conceptuses, and pregnant/non-pregnant endometrium. Interferon-γ inducible protein 10 kDa, (IP10) was found only at the endometrium of pregnant sheep uterus. In addition, about 400 genes that change their expression before and after implantation were determined by GeneChip analysis. Importance of those gene expressions is sorted out and relationships of those are being studied to understand the mechanism of implantation.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Imakawa, その他: "Co-expression of transforming growth factor β and interferon τ during peri-implantation period in the ewe."Endocrine Journal. 45. 441-450 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi, その他: "Identification of a functional AP-1 site in the ovine interferon-τ gene which Mediates PMA response in JEG3 cells."Biochemical Journal. 340. 767-773 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi, その他: "Identification of negative regulatory regions of interferon-τ gene."Endocrine Journal. 47. 137-142 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rider, その他: "Changes in the temporal and spatial expression of Hβ58 during formation and maturation of the chorioallantoic placenta in the rat."Biology of Reproduction. 63. 1735-1746 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshioka, その他: "Determination of genes involved in the process of implantation : Application of GeneChip to scan 6500 genes."Biochem.Biophys.Res.Commu.. 272. 531-538 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatama, その他: "Induction of reactivation of feline foamy virus in persistently infected feline cell lines by Trichostatin A."Virology. (In Press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imakawa, その他: "Cloned animal and placentation"Is the expression of ovine interferon-tau regulated by common transcriptional factors.. 78 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今川和彦 その他: "妊娠の生物学"着床とインターフェロン関連遺伝子. 320 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imakawa et al.: "Co-expression of transforming growth factor β and interferon τ during peri-implantation in the ewe"Endocrine Journal. 45. 441-450 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi et al.: "Effects of PMA and transcription factors on ovine interferon-τ transactivation in various cell lines"Endocrine Journal. 46. 383-388 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi et al.: "Identification of a functional AP-1 site in the ovine interferon-τ gene which mediates PMA response in JEG3 cells."Biochemical Journal. 340. 767-773 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aida et al.: "Identification of interferon-tau at the maternal-fetal interface in Shiba goats."J.Reproduction and Development. 45. 249-257 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi et al.: "Identification of negative regulatory regions of interferon-τ gene."Endocrine Journal. 47. 137-142 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rider et al.: "Changes in temporal and spatial expression of Hβ58 during formation and maturation of the chorioallantoic placenta in the rat."Biology of Reproduction. 63. 1735-1746 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi et al.: "genetic mapping of a locus associated with bovine chronic interstitial nephrites to chromosome 1."Animal Genetics. 31. 91-95 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka et al.: "Determination of genes involved in the process of implantation : Application of GeneChip to scan 6500 genes."Biopchem.Biophys.Res.Commu.. 272. 531-538 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagaoka et al.: "Implantation in ruminants : Changes in pre-implantation, maternal recognition of pregnancy, control of attachment and invasion.-Review-"Asian-Aus.J.Animal Science. 13. 845-855 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuraishi et al.: "The poly (A) tail length of casein mRNA in the lactating mammary gland changes depending upon the accumulation and removal of milk."Biochemical Journal. 347. 579-583 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi et al.: "Regulation of interferon-tau gene expression and maternal recognition of pregnancy.-Review-"J.Reproduction and Development. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatama et al.: "Induction of reactivation of feline foamy virus in persistently infected feline cell lines by Tricostatin A."Virology. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi et al.: "Enhancer regions of interferon-tau gene that confer PMA response or cell type specific transcription."Mol.Cell.Endocrinology. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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