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2000 Fiscal Year Final Research Report Summary

Molecular mechanism of fatty acid synthesis in plants and its application

Research Project

Project/Area Number 10460147
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNagoya University

Principal Investigator

SASAKI Yukiko  Graduate School of Agricultural Sciences, Nagoya University, Professor, 大学院・生命農学研究科, 教授 (00026519)

Project Period (FY) 1998 – 2000
KeywordsFatty acid biosynthesis / Acetyl-CoA carboxylase / Light-activation / Redox regulation / Pea plants
Research Abstract

Fatty acid synthesis in pea chloroplasts is regulated by light/dark. The regulatory enzyme, acetyl-CoA carboxylase (ACCase) which catalyzes the synthesis of malonyl-CoA, is modulated by light/dark, presumably under redox regulation. The plastidic ACCase is multienzyme complex composed of biotin carboxylase and carboxyltransferase (CT), and the latter enzyme is redox-regulated. The CT is composed of the nuclear-encoded α and the chloroplast-encoded β subunits, which contains 2 and 11 cysteine residues, respectively. To demonstrate the redox-regulation of this enzyme, we identified the cysteine residues involved in the regulation and and investigated the role of the two subunits. We expressed the recombinant CT in E.coli and found that the partly deleted CT was sensitive to redox state like the full-length CT.Site-directed mutagenesis of the deleted CT showed that replacement of a cysteine by alanine at the amino acid position 267 in the α polypeptide or at position 442 in the β polypeptide resulted in redox-insensitive CT and breaking intermolecular disulfide bond between the α and β polypeptides. Similar results were confirmed in the full-length CT.These results indicated that the two cysteines are involved in redox-regulation by intermolecular disulfide/dithiol exchange between the α and β subunits. This is the first demonstration that a covalent bond between nuclear-encoded polypeptide and chloroplast-encoded polypeptide regulates the enzyme activity.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Yukuko SASAKI: "Chloroplast RNA editing requiral for functional acetyl-CoA carborylase in plants"J.Biol.Chem.. 276. 3937-3940 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] AKIKO KOZAKI: "Recombinant carboxyltransferase responsive to redox of pea plastidic acetyl-CoA carboxylase"J.Biol.Chem.. 275. 10702-10708 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] AKIKO KOZAKI: "Light-dependent changes in redox status of the plastidic acetyl-CoA carboxylase and its regulatory component"Biochemical J.. 339. 541-546 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, A.and Sasaki, Y.: "Light-dependent changes in redox status of the plastidic acetyl-CoA carboxylase and its regulatory component."Biochem.J.. 339. 541-546 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kozaki, A., Kamada, K., Nagano, Y., Iguchi, H., and Sasaki, Y.: "Recombinant carboxyltransferase responsive to redox, of pea plastidic acetyl-CoA carboxylase."J.Biol.Chem.. 275. 10702-10708 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki, Y., Kozaki, A.Ohmori, A., Iguchi, H., and Nagano, Y.: "Chloroplast RNA editing required for functional acetyl-CoA carboxylase in plants."J.Biol.Chem.. 276 (in press). 3937-3940 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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