Mechanisms phototransduction and cGMP-dependent gene expressikon in plants

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 10460148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: KOBE UNIVERSITY
• Principal Investigator: YAMAGATA Hiroshi Kobe Univeristy, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00159203)
• Project Period (FY): 1998 – 2000
• Keywords: phototrunsduction / protein kinase / MAP kinase / cGMP / CDPK / soybean cell culture / phytochrome

Research Abstract

Light induced rapid and transient activation of a 46-kDa protein kinase in soybean photomixotrophic cell culture. This kinase was designated as LAP kinase (light signal-activated protein kinase). Activation of LAP kinase in response to light was associated with tyrosine phosphorylation of the kinase, and treatment of the kinase with protein tyrosine phosphatase abolished its activity. The LAP kinase efficiently phosphorylated myelin basic protein and histone, but did not phosphorylate casein. Phosphoamino acid analysis indicated that the LAP kinase was a serine/threonine protein kinase. These results indicated that the LAP kinase is related to the MAP kinase family of protein kinases.
To know the extensive general roles of cGMP on the regulation of plant gene expression, we have isolated several cGMP-regulated genes by PCR-based subtraction method from soybean cells. From the analyses of these genes, it was suggested that cGMP may control the synthesis of flavonoid phytoalexin and anthocyanin as well in plants.

Research Products

• [Publications] Yoshida I, Yamagata, H., Hirasawa, E.: "Signal transduction controlling the blue-and red-light mediated gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil."J.Exp.Bot.. (in press). (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamagata, H., Saka, K., Tanaka, T., Aizono, Y.: "Light activates a 46-kDa MAP kinase-like protein kinase in soybean cell culture"FEBS Letters. 494. 24-29 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida I, Yamagata, H., Hirasawa, E.: "Blue-and red-light regulation and circadian control of gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil."J.Exp.Bot.. 50・332. 319-326 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakayama, M., Masuda, T., Bando, T., Yamagata, H., Ohta, H., Takamiya, K.: "Cloning and expression of the soybean chlH gene encoding a subunit of Mg-chelatase and localization of the Mg concentration-dependent chl H protein within chroloplast"Plant Cell Physiol. 39・3. 275-284 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Frohnmeyer, H., Bowler, C., Xhu, G., Yamagata, H., Schafer, E., Chua N.-H.: "Different roles for calcium and calmodulin in phytochrome-and UV-regulated expression of chalcone synthase"Plant J.. 13・6. 763-772 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida, I., Yamagata, H., Hirasawa, E.: "Light-regulated gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil."J.Exp.Bot.. 46・326. 617-620 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamagata, H., Nakajima, A., Bowler, C., Iwasaki, T.: "Molecular cloning and characterization of a cDNA encoding asparagine synthetase from soybean (Glycine max L.) cell cultures"Biosci.Biotech.Biochem. 62・1. 148-150 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshida, I., Yamagata, H., and Hirasawa, E.: "Signal transduction controlling the blue- and red-light mediated gene expression of Sadenosylmethionine decaboxylase in Pharbitis nil"J. Exp. Bot.. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamagata, H., Saka, K., Tanaka, T. and Aizono, Y.: "Light activates a 46-kDa MAP kinase-like protein kinase in soybean cell culture"FEBS Lett.. 494. 24-29 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshida, I., Yamagata, H., and Hirasawa, E.: "Blue- and red-light regulation and circadian control of gene expression of S-adenosylmethoinine decarboxylase in Pharbitis nil"J. Exp. Bot.. 50(332). 319-326 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakayama, M., Masuda, T., Bando, T., Yamagata, H. Ohta, H., and Takamiya, K.: "Cloning and expression of the soybean ch1H gene encoding a subunit of Mg-chelatase and localizatiion of the Mg^<2+> concentration-dependent ch1H protein within the chloroplast"Plant Cell Physiol.. 39(3). 275-284 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Frohnmeyer, H., Bowler, C.,Xhu, G., Yamagata, H., Schafer, E. and Chua N.-H.: "Different roles for calcium and calmodulin in phytochrome- and UV-regulated expression of chalcone synthase"Plant J.. 13(6). 763-772 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshida, I., Yamagata, H., and Hirasawa, E.: "Light-regulated gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil."J. Exp. Bot.. 49(320). 617-620 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamagata, H., Nakajima, A.,Bowler, C., and Iwasaki, T.: "Molecular cloning and characterization of a cDNA encoding asparagine synthetase from soybean (Glycine max L.) cell cultures"Biosci. Biotech. Biochem. 62(1). 148-150 (1998)
  • Description: 「研究成果報告書概要(欧文)」より