• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

Structure and Functional Relationship of Band 3 Protein

Research Project

Project/Area Number 10470033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HAMASAKI Naotaka  Graduate School of Medical Sciences, Kyushu University, Department of Clinical Chemistry and Laboratory Medicine, Professor, 大学院・医学系研究科, 教授 (00091265)

Project Period (FY) 1998 – 1999
Keywordspolytopic membrane protein / biosynthesis of membrane protein / transmembrane peptide / signal anchor-I / transmembrane peptide / peptide interaction / band 3 protein / anion exchange
Research Abstract

Our recent studies on band 3 protein, a typical multi-spanning polytopic membrane protein, implied that some hydrophobicpeptideportions were found as free from boundary lipids even though their hydrophobicity was comparable to that of bound from (Hamasaki, et al., J. Biochem. 122, 577-585(1997)). Moreover, the anticipated transmembrane peptide portions of band 3 protein were not equivalent to each other and some of them had no topogenic signal activities (Ota, et al., Biol. Chem. 273, 28286-28291(1998)). It was also found that topogenic signal activities were affected by the mutual peptide-peptide interactions of the transmembrane peptide portions during biosynthesis in vitro (Ota, et al., Biol. Chem. 273, 28286-28291(1998)). Intriguingly, we also observed the evidence for a mode of co-translational insertion in which an internal signal-another sequence with Nexo/Ccyt topology conferred a transmembrane disposition onto a preceding hydrophilic peptide segment (Ota, et al., Molecular Cel … More l 2, 495-503(1998)), suggesting that hydrophilic peptide portions can transverse the membrane lipid bilayer surrounded by other trasnmembrane peptide portions (especially with amphipathic transmembrane peptide portions). It is highly probable to speculated that the mutual peptide-peptide interactions of multi-spanning polytopic membrane proteins occurred throughout the protein biosynthesis and plasma membrane intersection events (Hamasaki, et al., Biochem. Cell., Biol. 76, 729-733(1998)).
Based on this evidence, it can be concluded that the hydrophobic transmembrane peptide portions are not necessarily bound with boundary lipids in the membrane lipid bilayer. Thus, hydrophobicity is not an absolute requirement for the formation of a transmembrane segment in multispanning polytopic membrane proteins. Category 2 portions (see J. Biochem. 122, 577-585 (1997) ; Biochem. Cell Biol. 79, 729-733 (1998)) have considerable freedom in the membrane lipid bilayer. Proteins containing category 2 portions have a more flexible structure than membrane proteins that consist only of transmembrane peptide portions bound with boundary lipids. These flexible regions must play important roles in substrate-peptide interactions or ligand-mediated conformational change within membrane lipid bilayers. Further study on other polytopic membrane proteins would support this new concept. Less

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Naotaka Hamasaki: "The Role of Band 3 Protein in Oxygen Delivery by Red Blood Cells."Ind. J. Clin. Biochem.. 14(1). 49-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naotaka Hamasaki: "A New Conceptin Polytopic Membrane Proteins Following from the Study of Band 3 Protein"Biochem. Cell. Biol.. 76(5). 729-733 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 濱崎 直孝: "内在性膜蛋白質の新しいコンセプト"生物物理. 39(4). 240-245 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromicichi Mitsuyasu: "Dominant Effector Ile50 Val Variant of the Human IL-4 Receptora-Chain in IgE synthesis."J. Immunol.. 162. 1227-1234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Tsuda: "Screening for Aetiology of Thrombophilia: A High Prevalence of Protein S Abnormality."Ann. Clin. Biochem.. 36. 423-432 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideki Tatewaki: "A Novel Splice Acceptor Site Mutation Which Produces Multiple Splicing Abnormalities Resulting in Protein S Deficiency Type I."Thromb. Haemost.. 82(1). 65-71 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuma, H., Inoue, K., Fu., G., Ando, S., Lee, S., Sugihara, G., Hamasaki, N.: "Secondary Structure of Synthetic Peptides Corresponding to the First Membrane - Contact Portion of Normal Band 3 and its Deletion Mutant (Southeast Asian Ovalocytosis)"J. Biochem.. 124(3). 509-518 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota, K., Sakaguchi, M., Hamasaki, N., Mihara, K.: "Assessment of Topogenic Functions of Anticipated Transmembrane Segments of Human Band 3"J. Biol. Chem.. 273(43). 28286-28291 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ota, K., Sakaguchi M., von Heijine, G., Hamasaki, N., Mihara, K.: "Forced Transmembrane Orientation of Hydrophilic Polypeptide Segments in Multispanning Membrane Proteins"Molecular Cell. 2. 495-503 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamasaki N.: "The Role of Band 3 Protein in Oxygen Delivery by Red Blood Cells (Review Articles)"Ind. J. Clin. Biochem.. 14(1). 49-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamasaki N., Kuma, H., Kazuhisa Ota, K., Masao Sakaguchi, M., Mihara, K.: "A new Concept in Polytopic Membrane Proteins Following From the Study of Band 3 Protein"Biochem.Cell Biol.. 76. 729-733 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi