2001 Fiscal Year Final Research Report Summary
Molecular mechanism of persistent infection of SSPE virus
| Project/Area Number |
10470047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Niigata University |
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Principal Investigator |
ABE Satoshi Niigata University, Brain Research Institute , Assistant, 脳研究所, 助手 (90202663)
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| Co-Investigator(Kenkyū-buntansha) |
KUMANISHI Toshiro Niigata University, Brain Research Institute. , Professor, 脳研究所, 教授 (40018601)
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| Project Period (FY) |
1998 – 2000
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| Keywords | Subacutescrelosing panencephalitis / SSPE / Persistent infection / Gene mutation |
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| Research Abstract |
Subacute sclerosing panericephalitis (SSPE) is a chronic progressive disease of the central nervous system caused by a persistent infection of a variant of measles virus (SSPE virus). In this study, RT-PCR and CDNA cloning were used to examine the structural protein genes of SSPE virus in the brain and lymph node from a case ofSSPE. Six major structural protein genes (NP, P, M, F, HA and L) were detected in both brain and lymph node in our case. In the examination of the brain, multiple independent clones of the entire length of the coding region of the genes were sequenced.
The identical multiple mutations were revealed in the genes in comparison with Edmonston strain of measles virus. The lymph node clones had identical mutations with brain clones and had multiple additional mutations, indicating that the viral genes were still under the influence of the hypermutation mechanism. These findings suggest mat in addition to the central nervous system, the lymph node may also have a role in the progression of SSPE. Ninety-three percent of these mutations was transition (U to C). This biased hypermutation was considered to be specific for SSPE virus.
Translocations or chromosomal aberrations were not found in our case. Differentially expressed unique genes were not detected in the brain and lymph node by CDNA subtraction methods.
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