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1999 Fiscal Year Final Research Report Summary

Role of osteopoutin in homeostasis of bone tissue

Research Project

Project/Area Number 10470058
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOMURA Shintaro  OSAKA University Medical School, Associate Professor, 医学系研究科, 助教授 (80159087)

Project Period (FY) 1998 – 1999
Keywordsosteopontin / homeostasis / bone resorption / bone tissue / osteocyte / transcription / integrin / osleoclact
Research Abstract

Molecular mechanism of bone remodeling caused by mechanical stress is unknown. In this project, we identified osteopontin (Opn) as the trigger of bone remodeling. Experimental bone remodeling system developed by Waldo et al was used for the study. A 0.5mm diameter of elastic band was inserted interproximally among the upper first and second molar on both side. After 12 hours of treatment, Opn expression was detected in the osteocytes on the pressure side, and gradually spread to those on the tension side. Only 3% of the osteocytes located on the pressure side expressed Opn in the control rats. In contrast, the value was increased to 88% at 48 hoturs. Following the increased expression of Opn, a 17-fold greater number of osteoclasts compared with the control and numerous resorption pits were observed on the pressure side of the alveolar bone. Injection of RGDS-peptide strongly inhibited the increase in the number of osteoclasts. The results indicate that Opn is an important factor triggering bone remodeling caused by mechanical stress. Furthermore, Opn was identified as the crucial factor of the homeostasis of bone.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Sato M., Nomura S. et al.: "Microphthalmia-associated transcription factor (MITF) inleracts with PU.1 and c-fos : Detor mination of their subcellular localization."Biochem.Biophys.Ras.Commun.. 254. 384-387 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inada M., Nomura S. et al.: "Maturational disturbance of chondrocytes in Cbfa-1 deficient mice"Dev.Dynamics. 214. 279-290 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terai K., Nomura S. et al.: "The role of osteopontin in the bone remodeling caused by mechanical stress."J.Bone and Mineral Res.. 14. 839-849 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogibara H., Nomura S. et al: "Synergy of PEB2/CBF wills mi transcription factor (MITF) for transactivation of mouse mast cell profease 6 gene."Oncogene. 18. 4632-4639 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Maekawa, Nomura S. et al: "Mouse ATF2 null mutants display features of severe type of meconium aspiration syndrome"J.Biol.Chem.. 274. 17813-17819 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato M., Nomura S. et al.: "Osteopontin gene expression is regulated by cooperation of microphtholmice-associated transcription factor and PU.1 in osteoclasts"J.Bone and Mineral Res.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nomura et al: "Molecular Events Caused by Mechanical Stress in Bone (Matrix Biology)"Elsevier Science Inc. London(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sato, E.Morii, K.Takebayashi-Suzuki, N.Yasui, T.Ochi, Y.Kitamura amd S.Nomura.: "Microphthalmia-associated transcription factor (MITF) interactswith PU.1 and c-fos : determination of their subcellular localization."Biochem.Biophys.Res.Commn.. 254. 384-387 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Inada, T.Yasui, S.Nomura, S.Miyake, K.Deguchi, M.Himeno, M.Sato, H.Yamagiwa, T.Kimura, N.Yasui, T.Ochi, N.Endo, Y.Kitamura, T.Kishimoto and T.Komori.: "Maturational disturbance of chondrocytes in Cbfal-deficient mice."Dev.Dynamics. 214. 279-290 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Terai, T.Takano-Yamamoto, Y.Ohba, K.Hiura, M.Sugimoto, M.Sato, H.Kawahata, N.Inagura, Y.Kitamura and S.Nomura.: "The role of osteopontin in the bone remodeling caused by mechanical stress."J.Bone and Mineral Research.. 14. 839-849 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sato, T.Ochi, T.Nakase, S.Hirota, Y.Kitamura, S.Nomura and N.Yasui: "Mechanical tension-stress induces expression of BMP-2 and -4, butnot BMP-6, -7 and GDF-5 mRNA, during distraction osteogenesis."J.Bone and Mineral Research.. 14. 1084-1095 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasui, K.Fujita, M.Sato, M.Sugimoto, S.Hirota, Y.Kitamura, S.Nomura and K.Kohri: "Expression of bone matrix proteins in urolithiasis model rats."Urol.Res.. 27. 255-261 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ogihara, T.Kannno, E.Morii, D-K.Kim, Y-M.Lee, M.Sato, W-Y.Kim, S.Nomura, Y.Ito and Y.Kitamura: "Synergy of PEBP2/CBF with mi transcription factor (MITF) for transactivation of mouse mast cell protease 6 gene."Oncogene. 18. 4632-4639 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Maekawa, F.Bernier, M.Sato, S.Nomura, M.Singh, Y.Inoue, T.Tokunaga, H Imai, M.Yokoyama: "A.Reimond, L.Glimcher and S.Ishii. Mouse ATF2 null mutants display features of severe type of meconium aspiration syndrome."J.Biol.Chem.. 274. 17813-17819 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nomura and T.Takano-Yamamoto.: "Molecular events caused by mechanical stress in the bone."Matrix Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takeda, K.Obana, T.Mizusawa, Y.Tomita, K.Arai, T.Tsutsui, A.Hatano, K.Takahashi and S.Nomura: "Evidence for β_3-adrenoceptor subtypes in relaxiation of the human urinary baldder detrusor : Analysis by molecular biological pharmacological methods."J.of Pharmacol. and Exp.Therap.. 288. 1367-1373 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sato, E.Morii, K.Takebayashi-Suzuki, N.Yasui, T.Ochi, Y.Kitamura and S.Nomura.: "Osteopontin gene expression is regulated by cooperation of microphthalmia-associated transcription factor (MITF) and PU.1 in osteoclasts."J.Bone and Mineral Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E.Morii, H.Ogihara, T.Kanno, D-K.Kim, S.Nomura, Y.Ito and Y.Kitamura: "Identification of the region of mi transcription factor (MITF) which is responsible for the synergy with PEBP2/CBF."Biochem.Biophys.Res.Commn.. 261. 53-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Okazaki, S.Jingushi, T.Ikenoue, K.Urabe, H.Sakai, A.Ohtsuru, K.Akino, S.Yamashita, S.Nomura and Y.Iwamoto.: "Expression of insulin-like growth factor I messenger ribonucleic acid in developing osteophytes inmurine experimental osteoarthritis and in inoculated with growth hormne-secreting tumor."Endocrinology.. 140. 4821-4830 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Horikawa, S.Higashiyama, S.Nomura, Y.Kitamura, M.Ishikawa and N.Taniguchi.U: "pregulation of endogeneous heparin-binding EGF-like growth factor and its role as a survival factor in skeletal myotubes."FEBBS Lett.. 459. 100-104 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yasui, K.Fujita, M.Sato, M.Sugimoto, M.Iguchi, S.Nomura and K.Kohri.: "The effect of takusha, a kampo medicine, on renal stone formation and osteopontin expression in a rat urolithiasis model."Urol.Res.. 27. 194-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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