2000 Fiscal Year Final Research Report Summary
Identification of the cellular receptor for measles virus
| Project/Area Number |
10470079
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
YANAGI Yusuke Faculty of Medicine, KYUSHU UNIVERSITY, Professor, 大学院・医学研究院, 教授 (40182365)
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| Project Period (FY) |
1998 – 2000
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| Keywords | measles virus / receptor / SLAM / cell fusion |
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| Research Abstract |
Several years ago CD46 was identified as a cellular receptor for the Edmonston strain of measles virus, but most clinical isolates of measles virus, which are most efficiently isolated in marmoset B cell line B95a, cannot grow in many CD46^+ cell lines. Although some researchers attributed it to post-entry block in viral replication, others believed that there is a receptor other than CD46 for wild-type measles viruses. In this study, we showed that human SLAM (signaling lymphocytic activation molecule ; also known as CDw150), a recently discovered membrane glycoprotein involved in lymphocyte activation, is a cellular receptor for measles virus, including the Edmonston strain. Transfection with a human SLAM cDNA enabled nonsusceptible cell lines to bind measles virus, support measles virus replication, and develop cytopathic effects. The distribution of SLAM was consistent with the susceptibility of various cell lines to wild-type measles virus strains and with pathology of measles virus infection in humans and monkeys. By examining receptor function of various chimeric SLAM molecules, we also showed that the V domain of human SLAM is necessary and sufficient to interact with the measles virus H protein and allow measles virus entry. The identification of SLAM as a receptor for measles virus opens the way to a better understanding of pathogenesis of measles virus infection, especially measles virus-induced immunosuppression.
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Research Products
(10 results)
