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2000 Fiscal Year Final Research Report Summary

Studies of T cell Recognition for Viral Antigen Epitopes

Research Project

Project/Area Number 10470088
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionKumamoto University

Principal Investigator

TAKIGUCHI Masafumi  Center for AIDS Research, Kumamoto University, Professor, エイズ学研究センター, 教授 (00183450)

Co-Investigator(Kenkyū-buntansha) HIRAYAMA Kazuo  Cental Research Laboratories, Ajinomoto Co., Chief Researcher, 中央研究所, 主席研究員
TOMIYAMA Hiroko  Center for AIDS Research, Kumamoto University, Assistant Professor, エイズ学研究センター, 助手 (50301370)
Project Period (FY) 1998 – 2000
Keywordscytotoxic T cells / T cell receptor / HLA class I / peptide / HLA class I tetramer / CTL clone / 三次構造
Research Abstract

In the present studies, we demonstrated the 3-dimensional structure of HLA class I・peptide complex recognized by cytotoxic T lymphocytes(CTL) and investigated CTL recognition ex vivo using HLA class I tetramers.
(1) We demonstrated the 3-dimensional structure of two HLA-B^*5101-HIV-1 epitope peptide complexes. The structure were compared to those of HLA-B^*3501 and HLA-B^*5301.
(2) We established methods to detect antigen-specific CD8^+ T cells using HLA class I tetramers. 11HIV-1 epitopes, one HCV epitope and two HBV epitopes were used to make the tetramers. These tetramers were useful to detect epitope-specific CD8^+ T cells in PBMC from patients infected with HIV, HCV or HBV.
(3) We demonstrated increase of HIV-1 specific CD28^-CD45RA^- CD8^+ T cells in PBMC of chronically HIV-1 infected individuals and HCV-specific CD28^+CD45RA^-CD8^+ T cells in PBMC of individuals with acute HCV infection
(4) We demonstrated that a single CTL clone recognize a single peptide presented by two different HLA class I, HLA-B^*5101 and HLA-B^*3501.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Masaaki Ibe: "Identification and characterization of a cytotoxic T cell epitope of hepatitis C virus presented by HLA-B^*3501 in acute hepatitis"J.Gen.Virol.. 79. 1735-1744 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuki Ikeda-Moore: "Identification of a novel HLA-A24-restricted CTL epitope derived from HIV-1 Gag protein"AIDS. 12. 2073-2074 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dariusz Kmieciak: "The effect of epitope variation on the profile of cytotoxic T lymphocyte responses to the HIV envelope glycoprotein."Int.Immunol.. 10. 1789-1799 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Tomiyama: "Identification of Multiple HIV-1 Epitopes presented by HLA-B^*5101 Molecules."Hum.Immunol.. 60. 177-186 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Tomiyama: "Cytotoxic T-lymphocyte recognition of HLA-B^*5101-restricted HLV-1 Rev epitope which is naturally processed in HIV-1 infected cells."AIDS. 13. 861-863 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masataka Oiso: "A newly identified MAGE-3-derived epitope recognized by HLA-A24 restricted cytotoxic T lymphocytes."Int.J.Cancer.. 81. 387-394 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuhiko Fukada: "HLA-A^*1101-restricted CTL recognition of HIV-1 Pol protein."AIDS. 13. 1413-1414 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuhiko Fukada: "HLA-A^*1101-restricted CTL recognition for a novel epitope derived from the HIV-1 Env protein."AIDS. 13. 2597-2599 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Tomiyama: "Expansion of HIV-1-specific CD28^-CD45RA^-CD8^+T cells in chronically HIV-1 infected individuals."AIDS. 14. 2049-2051 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroko Tomiyama: "Direct evidence for dual recognition by a single CTL clone of an HIV-1 Nonamer epitope presented by two different HLA class I moleculed."Eur.J.Immunol.. 30. 2521-2530 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsumi Maenaka: "Nonstandard petide binding revealed by crystal structures of HLA-B^*5101 complexed with HIV immunodominant epitopes."J.Immunol.. 165. 3268-3274 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Sobao: "Visual demonstration of HCV-specific memory CD8^+ T cell expansion in patients with acute hepatitis C."Hepatology.. 33. 287-294 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaaki Ibe, Takashi Sakaguchi, Katsuaki Tanaka, Satoru Saito, Shumpei Yokota, Torahiko Tanaka, Kunitada Shimotohno, Yoshitomo Chujoh, Yasushi Shiratori, Masao Omata, Kiyoshi Miwa and Masafumi Takiguchi: "Identification and characterization of a cytotoxic T cell epitope of hepatitis C virus presented by HLA-B^*3501 in acute hepatitis."J.Gen.Virol.. 79. 1735-1744 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuki Ikeda-Moore, Hiroko Tomiyama, Masaaki Ibe, Shinichi Oka, Kiyoshi Miwa, Yutaro kaneko and Masafumi Takiguchi: "Identification of a novel HLA-A24-restricted CTL epitope derived from HIV-1 Gag protein."AIDS. 12. 2073-2074 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dariusz Kmieciak, Iiona Bednarek, Masafumi Takiguchi, Thomas J.Wasik, Jolanta Bratosiewicz, Andrzej Wierzbicki, Hedy Teppler, Janet Pientka, Susan H.Hsu, Yutaro Kaneko and Danuta Kozbor: "The effect of epitope variation on the profile of cytotoxic T lymphocyte responses to the HIV envelope glycoprotein."Int.Immunol.. 10. 1789-1799 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroko Tomiyama, Takashi Sakaguchi, Kiyoshi Miwa, Shinichi Oka, Aikichi Iwamoto, Yutaro Kaneko and Masafumi Takiguchi: "Identification of Multiple HIV-1 Epitopes presented by HLA-B^*5101 Molecules."Hum.Immunol.. 60. 177-186 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroko Tomiyama, Yoshitomo Chujoh, Tatsuo Shioda, Kioyoshi Miwa, Shinichi Oka, Yutaro Kaneko and Masafumi Takiguchi: "Cytotoxic T-lymphocyte recognition of HLA-B^*5101-restricted HIV-1 Rev epitope which is naturally processed in HIV-1 infected cells."AIDS.. 13. 861-863 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masatake Oiso, Masao Eura, Fumihiro Katsura, Masafumi Takiguchi, Yuji Sobao, Keisuke Massuyama, Manabu Nakashima Kyogo Itoh and Takeru Ishikawa: "A newly identified MAGE-3-derived epitope recognized by HLA-A24 restricted cytotoxic T lymphocytes."Int.J.Cancer.. 81. 387-394 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko Fukada, Yoshitomo Chujoh, Hiroko Tomiyama, Kiyoshi Miwa, Yutaro Kaneko, Shinichi Oka and Masafumi Takiguchi: "HLA-A^*1101-restricted CTL recognition of HIV-1 Pol protein."AIDS.. 13. 1413-1414 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko Fukada, Hiroko Tomiyama, Yoshitomo Chujoh, Kiyoshi Miwa, Yutaro Keneko, Shinichi Oka and Masafumi Takiguchi: "HLA-A^*1101-restricted CTL recognition for a novel epitope derived from HIV-1 Env protein."AIDS.. 13. 2597-2599 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroko Tomiyama, Shinichi Oka, Graham S.Ogg, Setsuko Ida, Andrew J.McMichael and Masafumi Takiguchi: "Expansion of HIV-1-specific CD28^-CD45RA^-CD8^+T cells in chronically HIV-1 infected individuals."AIDS.. 14. 2049-2051 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroko Tomiyama, Naoyuki Yamada, Hiroki Komatsu, Kazuo Hirayama and Masafumi Takiguchi: "A single CTL clone can recognize a naturally processed HIV-1 epitope presented by two different HLA class I molecules."Eur.J.Immunol.. 30. 2521-2530 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsumi Maenaka, Takeko Maenaka, Hiroko Tomiyama, Masafumi Takiguchi, David I.Stuart and E.Yvonne Jones: "Nonstandard peptide binding revealed by cystal structures of HLA-B^*5101 complexed with HIV immunodominant epitopes."J.Immunol.. 165. 3268-3274 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuji Sobao, Hiroko Tomiyama, Saburo Nakamura, Hisahiko Sekihara, Katsuaki Tanaka, and Masafumi Takiguchi: "Visual demonstration of HCV-specific memory CD8^+ T cell expansion in patients with acute hepatitis C."Hepatology.. 33. 287-294 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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