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2000 Fiscal Year Final Research Report Summary

ESTIMATION OF POSTMORTEM INTERVAL BY POSTMORTEM BIOCHEMICAL CHANGES

Research Project

Project/Area Number 10470118
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionUNIVERSITY OF TSUKUBA

Principal Investigator

MISAWA Shogo  INSTITUTE OF COMMUNITY MEDICINE, UNIVERSITY OF TSUKUBA, PROFESSOR, 社会医学系, 教授 (50086534)

Co-Investigator(Kenkyū-buntansha) HONDA Katsuya  COURSE OF SOCIAL MEDICINE, OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (00240789)
INOMATA Shinich  INSTITUTE OF CLINICAL MEDICINE, UNIVERSITY OF TSUKUBA, ASSISTANT PROFESSOR, 臨床医学系, 講師 (10282352)
TANAKA Einosuke  INSTITUTE OF COMMUNITY MEDICINE, UNIVERSITY OF TSUKUBA, ASSISTANT PROFESSOR, 社会医学系, 講師 (30138416)
TSUCHIYA Terumasa  MEDICAL RESEARCH INSTITUTE, TOKYO MEDICAL AND DENTAL UNIVERSITY, ASSISTANT, 難治疾患研究所, 助手 (20242109)
KOMINATO Yoshihiko  FACULLY OF MEDICINE, TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, ASSISTANT PROFESSOR, 医学部, 講師 (30205512)
Project Period (FY) 1998 – 2000
Keywordspost-mortem interval / vascular endothelial growth factor (VEGF) / hypoxia / forensic science / ウェスタン・ブロッティング
Research Abstract

Estimation of the post-mortem interval (PMI) is one of the most important tasks in forensic medicine. Five autopsy organ tissues such as brain, lungs, heart, liver and kidneys were taken at the time of forensic autopsy from 20 known PMI cases with a range of postmortem intervals ranging from 1 to 120 hrs (the mean was 25.81 hrs) and the time-course of vascular endothelial growth factor (VEGF) expression was measured. The human hepatoma-derived Hep 3B cell line was used as a control.
The levels of VEGF increased linearly with the PMI up to 20 hrs in lung (r=0.95 and in kidney (r=0.89), and up to 15 hrs PMI in liver (r=0.88). The VEGF levels fell after 24 hrs PMI, and then remained stable. In brain, the levels of VEGF started to increase after 24 hrs PMI and increased linearly with PMI up to 40 hrs in brain (r=0.94) and then begin to fall. In heart, there was no clear correlation between the PMI and VEGF level. Some variations occurred in selected cases, such as the infant and asphyxial deaths.
In conclusion, measurement of hypoxia-inducible levels of VEGF in various body organs appears to be a useful method of estimating the PMI up to 24 hrs in forensic medicine and pathophysiology. This method is alsoprobably applicable in ischaemia in clinical and basic medicine.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Katsuya Honda: "Quantitative ultrasonic tissue characterization can identify high-risk atherosclerotic alteration in human carotid arteries."Circulation. 102. 766-770 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiko Kominato: "Murine equivalent of the human histo-blood group ABO gene is a cis-AB gene and encodes a gylcosyltransferase with both A and B transferase activity."Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terumasa Tsuchiya: "Sensory Mechanism of Oxygen Sensor FixL From Rhizobium meliloti : Crystallographic, Mutagenesis and Resonance Raman Spectroscopic Studies."J.Mol.Biol.. 301. 415-431 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shogo Misawa: "Chlorzoxazone : a probe drug whose metabolism can used to monitor toluene exposure in rats."Arch Toxicol. 74. 139-144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shogo Misawa: "Quantitative analysis of pulmonary neuroendocrine cell distribution of the fetal small airways using double-labeled immunohistochemistry."Forensic Science International. 113. 183-187 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Einosuke Tanaka: "Differences in chlorzoxazone metabolism in full mature male and female Sprague-Dawley rat, Beagle dog and Cynomolgus monkey."Human & Experimental Toxicology. 19. 122-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2002-03-26  

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