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2000 Fiscal Year Final Research Report Summary

Application of solid-phase microextraction/high-performance liquid chromatography mass spectrometry to forensic toxicology

Research Project

Project/Area Number 10470122
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionShowa University

Principal Investigator

KUMAZAWA Takeshi  Showa University, School of Medicine, Associate Professor, 医学部, 助教授 (00186470)

Co-Investigator(Kenkyū-buntansha) SUZUKI Osamu  Hamamatsu University, School of Medicine, Professor, 医学部, 教授 (70093044)
SATO Keizo  Showa University, School of Medicine, Professor, 医学部, 教授 (20162422)
LEE Xiao-pen  Showa University, School of Medicine, Assistant Professor, 医学部, 講師 (90245829)
Project Period (FY) 1998 – 2000
KeywordsSolid-phase microextraction / SPME / drugs and poisons / high performance liquid chromatography / Mass spectrometry
Research Abstract

In forensic toxicology, the extraction, identification and quantification of a chemical (s) are essential in the case of victims whose case of death is considered due to or related to intoxication by drugs and poisons. The main interest in these fields is focused on methodologies, with regard to how rapidly, accurately and sensitively chemicals can be detected. In the present study, we established a procedure for the determination of stimulants, opioids, phenothiazines, butyrophenones, benzodiazepines, organophosphate pesticides, carbamate pesticides and naturally occurring poisons in human samples by solid-phase microextraction (SPME)-high performance liquid chromatography (HPLC)/mass spectrometry (MS).
SPME was achieved with polydimethylsiloxane (PDMS) for stimulants or organophosphate pesticides, carbowax/divinylbenzene (DVB) for opioids, polyacrylate for phenothiazines or butyrophenones and PDMS/DVB for benzodiazepines, carbamate pesticides or amanitins from human whole blood, serum and urine samples. HPLC/MS with electrospray ionization (ESI) as interface and ionization mode was used for detection of all drugs and poisons, except for atmospheric pressure chemical ionization for detection of organophosphate pesticides. In the analysis of phenothiazines, butyrophenones and benzodiazepines, HPLC/tandem MS (MS/MS) showed much higher sensitivity than HPLC/MS.
In addition to the spiked human body fluids, the method was applied to urine samples containing methamphetamine and amphetamine in a medico-legal case, and to samples of whole blood and urine after oral administration of phenothiazines by a male volunteer, these drugs could be isolated from human samples by SPME and sensitively quantified by HPLC/MS or HPLC/MS/MS.The present SPME-HPLC/MS method can be recommended for use in therapeutic monitoring, clinical toxicology and forensic toxicology.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Takeshi Kumazawa: "Extraction of methylxanthines from human body fluids by solid-phase microextraction"Anal.Chim.Acta. 387. 53-60 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Kumazawa: "Determination of triazine herbicides in human body fluids by solid-phase microextraction and capillary gas chromatography"Chromatographia. 52. 195-199 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Kumazawa: "Determination of phenothiazines in human body fluids by solid-phase microextraction and liquid chromatography/tandem mass spectrometry"J.Mass Spectrom.. 35. 1091-1099 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Kumazawa: "Separation methods for amino group-possessing pesticides in biological samples"J.Chromatogr.B. 747. 241-254 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 熊澤武志: "生体試料からの薬毒物の抽出法"臨床検査. 44. 1514-1524 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Seno: "Analyses of butyrophenones and their analogues in whole blood by high-performance liquid chromatography-electrospray tandem mass spectrometry"J.Chromatogr.B. 746. 3-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi Kumazawa: "Extraction of methylxanthines from human body fluids by solid-phase microextraction."Anal.Chem.Acta. 387. 53-60 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi Kumazawa: "Determination of triazine herbicides in human body fluids by solid-phase microextraction."Chromatographia. 52. 195-199 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi Kumazawa: "Determination of phenothiazines in human body fluids by solid-phase microextraction and liquid chromatography/tandem mass spectrometry."J.Mass Spectrom.. 35. 1091-1099 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi Kumazawa: "Separation methods for amino group-possessing pesticides in biological samples."J.Chromatogr.B. 747. 241-254 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi Kumazawa: "Extraction of drugs and poisons from biological samples."J.Med.Technol.. 44. 1514-1524 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Seno: "Analysis of butyrophenones and their analogues in whole blood by high-performance liquid chromatography-electrospray tandem mass spectrometry."J.Chromatogr.B. 746. 3-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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