1999 Fiscal Year Annual Research Report

C型肝炎ウイルスによる肥大型心筋症発症機序の解明

Research Project

Project/Area Number	10470164
Research Institution	KYOTO UNIVERSITY
Principal Investigator	松森昭京都大学, 大学院・医学研究科, 助教授 (70135573)
Keywords	C型肝炎ウイルス / 心筋症 / 肥大型心筋症 / 心筋炎 / HLA / ウイルスゲノム / 遺伝子
Research Abstract	【目的】WHO/世界心臓連盟の国際共同研究として22例の心筋炎または拡張型心筋症(DCM)の心筋標本を用いてウイルスゲノムの検索を行い、6例(27%)のC型肝炎ウイルス(HCV)を検出し、検出頻度はHCVが最も高かった。ホルマリン固定、パラフィン切片からのHCVゲノムの検出法を確立し、1973-1997年の心筋症、心筋炎系106例の剖検心および対照群として心筋梗塞35例、非心臓疾患C型肝炎ウイルス20例の計55例を用いてHCVゲノムの検出を行った。β-アクチン陽性例のうち心筋炎では12例中4例(33.3%、対照群0%、p=0.02)、DCMでは26例中3例(11.5%、p=0.27)、肥大型心筋症(HCM)では23例中6例(26.0%、p=0.03)と、HCVゲノムを高率に検出した。また、ユタ大学との共同研究において1985-1997年のDCMの剖検心103例中β-アクチンmRNAを38例に認め、このうち、9例(23.7%)にHCVゲノムを検出した。これらのPCR産物の塩基配列はHCVの標準株と90%以上のホモロジーがあり、剖検心から検出されたRNAはHCV由来のものであることが確認された。HCV心筋症とHLA遺伝子との関連を明らかにするため、HCV感染を伴うDCM(n=19)ではHLA-A11,B67,DPB1^0901,HCM(n=12)ではHLA-DB1^0407,DRB1^0901,DQB1^0303と関連することが明らかとなり、宿主の免疫応答性が臨床病型に影響する可能性が示唆されている。

Research Products
(13 results)

All Other

All Publications (13 results)

[Publications] Furukawa Y.et al.: "Anti-monocyte chemoattractant protein-1/monocyte chemotactic and activating factor antibody inhibits neointimal hyperplasia in injured rat carotid arteries"Circ Res. 84. 306-314 (1999)
[Publications] Ono K.et al.: "Prevention of myocardial reperfusion injury in rats by an antibody against monocyte chemotactic and activating factor/monocyte chemoattractant protein-1"Lab Invest. 79. 195-203 (1999)
[Publications] Okada M.et al.: "Hepatocyte growth factor is a major mediator in heparin-induced angiogenesis"Biochem Biophys Res Commun. 255. 80-87 (1999)
[Publications] Iwasaki A.et al.: "Pimobendan inhibits the production of proinflammatory cytokines and gene expression of inducible nitric oxide synthase in a murine model of viral myocarditis"J Am Coll Cardiol. 33. 1400-1407 (1999)
[Publications] Matsumori A.et al.: "Apical hypertrophic cardiomyopathy and hepatitis C virus infection"Jpn Circ J. 63. 433-438 (1999)
[Publications] Hwang MW.et al.: "FTY720, an new immunosuppressant, promotes long-term graft survival and inhibits the progression of graft coronary artery disease in a murine model of cardiac transplantation"Circulation. 100. 1322-1329 (1999)
[Publications] Hara M.et al.: "Mast cells apoptosis of cardiomyocytes and proliferation of other intramyocardial cells in vivo"Circulation. 100. 1443-1449 (1999)
[Publications] Nishio R.et al.: "Treatment of experimental viral myocarditis with interleukin-10"Circulation. 100. 1102-1108 (1999)
[Publications] Ono K.et al.: "Contribution of endothelin-1 to myocardial injury in a murine model of myocarditis.Acute effects of bosentan, an endothelin receptor antagonist"Circulation. 100. 1823-1829 (1999)
[Publications] Furukawa Y.et al.: "Cytokine gene expression during the development of graft coronary artery disease in mice"Jpn Circ J. 63. 775-782 (1999)
[Publications] Matsumori A.et al.: "High doses of digitalis increase the myocardial production of proinflammatory cytokines and worsen myocardial injury in viral myocarditis: A possible mechanism of digitalis toxicity"Jpn Circ J. 63. 934-940 (1999)
[Publications] Gwathmey JK.et al.: "Cellular and molecular remodeling in an animal model of dilated cardiomyopathy treated with the b-blocker carteolol"Am J Physiol. 276. H1678-H1690 (1999)
[Publications] Ono K, Matsumori A.: "Inflammatory heart disease."In Congestive heart failure, second Edition, edited by Jeffrey D. Hosenpud and Barry H. Grenberg. Lippincott Williams & Wilkins, Philadelphia.. 11 (1999)

1999 Fiscal Year Annual Research Report

C型肝炎ウイルスによる肥大型心筋症発症機序の解明

Principal Investigator

松森 昭 京都大学, 大学院・医学研究科, 助教授 (70135573)

Research Products

[Publications] Furukawa Y.et al.: "Anti-monocyte chemoattractant protein-1/monocyte chemotactic and activating factor antibody inhibits neointimal hyperplasia in injured rat carotid arteries"Circ Res. 84. 306-314 (1999)

[Publications] Ono K.et al.: "Prevention of myocardial reperfusion injury in rats by an antibody against monocyte chemotactic and activating factor/monocyte chemoattractant protein-1"Lab Invest. 79. 195-203 (1999)

[Publications] Okada M.et al.: "Hepatocyte growth factor is a major mediator in heparin-induced angiogenesis"Biochem Biophys Res Commun. 255. 80-87 (1999)

[Publications] Iwasaki A.et al.: "Pimobendan inhibits the production of proinflammatory cytokines and gene expression of inducible nitric oxide synthase in a murine model of viral myocarditis"J Am Coll Cardiol. 33. 1400-1407 (1999)

[Publications] Matsumori A.et al.: "Apical hypertrophic cardiomyopathy and hepatitis C virus infection"Jpn Circ J. 63. 433-438 (1999)

[Publications] Hwang MW.et al.: "FTY720, an new immunosuppressant, promotes long-term graft survival and inhibits the progression of graft coronary artery disease in a murine model of cardiac transplantation"Circulation. 100. 1322-1329 (1999)

[Publications] Hara M.et al.: "Mast cells apoptosis of cardiomyocytes and proliferation of other intramyocardial cells in vivo"Circulation. 100. 1443-1449 (1999)

[Publications] Nishio R.et al.: "Treatment of experimental viral myocarditis with interleukin-10"Circulation. 100. 1102-1108 (1999)

[Publications] Ono K.et al.: "Contribution of endothelin-1 to myocardial injury in a murine model of myocarditis.Acute effects of bosentan, an endothelin receptor antagonist"Circulation. 100. 1823-1829 (1999)

[Publications] Furukawa Y.et al.: "Cytokine gene expression during the development of graft coronary artery disease in mice"Jpn Circ J. 63. 775-782 (1999)

[Publications] Matsumori A.et al.: "High doses of digitalis increase the myocardial production of proinflammatory cytokines and worsen myocardial injury in viral myocarditis: A possible mechanism of digitalis toxicity"Jpn Circ J. 63. 934-940 (1999)

[Publications] Gwathmey JK.et al.: "Cellular and molecular remodeling in an animal model of dilated cardiomyopathy treated with the b-blocker carteolol"Am J Physiol. 276. H1678-H1690 (1999)

[Publications] Ono K, Matsumori A.: "Inflammatory heart disease."In Congestive heart failure, second Edition, edited by Jeffrey D. Hosenpud and Barry H. Grenberg. Lippincott Williams & Wilkins, Philadelphia.. 11 (1999)

松森昭京都大学, 大学院・医学研究科, 助教授 (70135573)